Candida albicans and Enterococcus faecalis biofilm frenemies: When the relationship sours
The opportunistic yeast Candida albicans and lactic acid bacteria Enterococcus faecalis are frequently co-isolated from various infection sites on the human body, suggesting a common interkingdom interaction. While some reports suggest an antagonism, the reason for their co-isolation therefore remai...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biofilm |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207522000065 |
| _version_ | 1811201553207394304 |
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| author | Om Alkhir Alshanta Khawlah Albashaireh Emily McKloud Christopher Delaney Ryan Kean William McLean Gordon Ramage |
| author_facet | Om Alkhir Alshanta Khawlah Albashaireh Emily McKloud Christopher Delaney Ryan Kean William McLean Gordon Ramage |
| author_sort | Om Alkhir Alshanta |
| collection | DOAJ |
| description | The opportunistic yeast Candida albicans and lactic acid bacteria Enterococcus faecalis are frequently co-isolated from various infection sites on the human body, suggesting a common interkingdom interaction. While some reports suggest an antagonism, the reason for their co-isolation therefore remains unclear. The purpose of this study was to undertake a detailed characterisation of this dual-species interaction. We used standard biofilm characterisation methodologies alongside an RNASeq analysis to assess the response of C. albicans to E. faecalis. We evaluated the relevance of pH to dual-species biofilm interactions and demonstrated that E. faecalis rapidly and significantly impacted C. albicans morphogenesis and biofilm formation, which was mirrored by levels of gene expression. These transcripts were enriched in amino acids biosynthesis and metabolism pathways in co-cultures, a finding that guided our investigation into pH related mechanism. We were able to demonstrate the direct role of E. faecalis induced low pH, which inhibited C. albicans hyphal morphogenesis and biofilm formation. The results suggest that the anti-candidal effect of E. faecalis is not based solely on a single mechanism, instead it may involve various mechanisms, which collectively reflects the complexity of interaction between C. albicans and E. faecalis and impacts treatment outcomes. |
| first_indexed | 2024-04-12T02:23:25Z |
| format | Article |
| id | doaj.art-922e0394ede0423aabecd1357b5bd172 |
| institution | Directory Open Access Journal |
| issn | 2590-2075 |
| language | English |
| last_indexed | 2024-04-12T02:23:25Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj.art-922e0394ede0423aabecd1357b5bd1722022-12-22T03:52:04ZengElsevierBiofilm2590-20752022-12-014100072Candida albicans and Enterococcus faecalis biofilm frenemies: When the relationship soursOm Alkhir Alshanta0Khawlah Albashaireh1Emily McKloud2Christopher Delaney3Ryan Kean4William McLean5Gordon Ramage6Glasgow Endodontology and Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, Glasgow, United KingdomGlasgow Endodontology and Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, Glasgow, United KingdomGlasgow Endodontology and Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, Glasgow, United KingdomGlasgow Endodontology and Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, Glasgow, United KingdomDepartment of Biological and Biomedical Sciences, School of Health and Life Sciences, Glasgow Caledonian University, Glasgow, United KingdomGlasgow Endodontology and Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, Glasgow, United KingdomGlasgow Endodontology and Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, Glasgow, United Kingdom; Corresponding author.The opportunistic yeast Candida albicans and lactic acid bacteria Enterococcus faecalis are frequently co-isolated from various infection sites on the human body, suggesting a common interkingdom interaction. While some reports suggest an antagonism, the reason for their co-isolation therefore remains unclear. The purpose of this study was to undertake a detailed characterisation of this dual-species interaction. We used standard biofilm characterisation methodologies alongside an RNASeq analysis to assess the response of C. albicans to E. faecalis. We evaluated the relevance of pH to dual-species biofilm interactions and demonstrated that E. faecalis rapidly and significantly impacted C. albicans morphogenesis and biofilm formation, which was mirrored by levels of gene expression. These transcripts were enriched in amino acids biosynthesis and metabolism pathways in co-cultures, a finding that guided our investigation into pH related mechanism. We were able to demonstrate the direct role of E. faecalis induced low pH, which inhibited C. albicans hyphal morphogenesis and biofilm formation. The results suggest that the anti-candidal effect of E. faecalis is not based solely on a single mechanism, instead it may involve various mechanisms, which collectively reflects the complexity of interaction between C. albicans and E. faecalis and impacts treatment outcomes.http://www.sciencedirect.com/science/article/pii/S2590207522000065InterkingdomBiofilmCandida albicansEnterococcus faecalisSupernatantpH |
| spellingShingle | Om Alkhir Alshanta Khawlah Albashaireh Emily McKloud Christopher Delaney Ryan Kean William McLean Gordon Ramage Candida albicans and Enterococcus faecalis biofilm frenemies: When the relationship sours Biofilm Interkingdom Biofilm Candida albicans Enterococcus faecalis Supernatant pH |
| title | Candida albicans and Enterococcus faecalis biofilm frenemies: When the relationship sours |
| title_full | Candida albicans and Enterococcus faecalis biofilm frenemies: When the relationship sours |
| title_fullStr | Candida albicans and Enterococcus faecalis biofilm frenemies: When the relationship sours |
| title_full_unstemmed | Candida albicans and Enterococcus faecalis biofilm frenemies: When the relationship sours |
| title_short | Candida albicans and Enterococcus faecalis biofilm frenemies: When the relationship sours |
| title_sort | candida albicans and enterococcus faecalis biofilm frenemies when the relationship sours |
| topic | Interkingdom Biofilm Candida albicans Enterococcus faecalis Supernatant pH |
| url | http://www.sciencedirect.com/science/article/pii/S2590207522000065 |
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