Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity
The authors describe programmable Argonaute nucleases that use small DNA guides to recognize and cleave RNA targets and can sense mismatches and modifications in RNA targets, making them potential tools for RNA biotechnology.
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-32079-5 |
| _version_ | 1818499049547890688 |
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| author | Lidiya Lisitskaya Yeonoh Shin Aleksei Agapov Anna Olina Ekaterina Kropocheva Sergei Ryazansky Alexei A. Aravin Daria Esyunina Katsuhiko S. Murakami Andrey Kulbachinskiy |
| author_facet | Lidiya Lisitskaya Yeonoh Shin Aleksei Agapov Anna Olina Ekaterina Kropocheva Sergei Ryazansky Alexei A. Aravin Daria Esyunina Katsuhiko S. Murakami Andrey Kulbachinskiy |
| author_sort | Lidiya Lisitskaya |
| collection | DOAJ |
| description | The authors describe programmable Argonaute nucleases that use small DNA guides to recognize and cleave RNA targets and can sense mismatches and modifications in RNA targets, making them potential tools for RNA biotechnology. |
| first_indexed | 2024-12-10T20:23:59Z |
| format | Article |
| id | doaj.art-922fcc64ae404c05b9dbd574e4b247a5 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-10T20:23:59Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-922fcc64ae404c05b9dbd574e4b247a52022-12-22T01:34:56ZengNature PortfolioNature Communications2041-17232022-08-0113111510.1038/s41467-022-32079-5Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificityLidiya Lisitskaya0Yeonoh Shin1Aleksei Agapov2Anna Olina3Ekaterina Kropocheva4Sergei Ryazansky5Alexei A. Aravin6Daria Esyunina7Katsuhiko S. Murakami8Andrey Kulbachinskiy9Institute of Molecular Genetics, National Research Center “Kurchatov Institute”Department of Biochemistry and Molecular Biology, Pennsylvania State UniversityInstitute of Molecular Genetics, National Research Center “Kurchatov Institute”Institute of Molecular Genetics, National Research Center “Kurchatov Institute”Institute of Molecular Genetics, National Research Center “Kurchatov Institute”Institute of Molecular Genetics, National Research Center “Kurchatov Institute”Division of Biology and Biological Engineering, California Institute of TechnologyInstitute of Molecular Genetics, National Research Center “Kurchatov Institute”Department of Biochemistry and Molecular Biology, Pennsylvania State UniversityInstitute of Molecular Genetics, National Research Center “Kurchatov Institute”The authors describe programmable Argonaute nucleases that use small DNA guides to recognize and cleave RNA targets and can sense mismatches and modifications in RNA targets, making them potential tools for RNA biotechnology.https://doi.org/10.1038/s41467-022-32079-5 |
| spellingShingle | Lidiya Lisitskaya Yeonoh Shin Aleksei Agapov Anna Olina Ekaterina Kropocheva Sergei Ryazansky Alexei A. Aravin Daria Esyunina Katsuhiko S. Murakami Andrey Kulbachinskiy Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity Nature Communications |
| title | Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity |
| title_full | Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity |
| title_fullStr | Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity |
| title_full_unstemmed | Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity |
| title_short | Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity |
| title_sort | programmable rna targeting by bacterial argonaute nucleases with unconventional guide binding and cleavage specificity |
| url | https://doi.org/10.1038/s41467-022-32079-5 |
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