Screening of Hydroxyapatite Biomaterials for Alveolar Augmentation Using a Rat Calvaria Critical-Size Defect Model: Bone Formation/Maturation and Biomaterials Resolution
Background: Natural (bovine-/equine-/porcine-derived) or synthetic hydroxyapatite (HA) biomaterials appear to be the preferred technologies among clinicians for bone augmentation procedures in preparation for implant dentistry. The aim of this study was to screen candidate HA biomaterials intended f...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/11/1677 |
| _version_ | 1797465856881459200 |
|---|---|
| author | Cristiano Susin Jaebum Lee Tiago Fiorini Ki-Tae Koo Peter Schüpbach Amanda Finger Stadler Ulf ME Wikesjö |
| author_facet | Cristiano Susin Jaebum Lee Tiago Fiorini Ki-Tae Koo Peter Schüpbach Amanda Finger Stadler Ulf ME Wikesjö |
| author_sort | Cristiano Susin |
| collection | DOAJ |
| description | Background: Natural (bovine-/equine-/porcine-derived) or synthetic hydroxyapatite (HA) biomaterials appear to be the preferred technologies among clinicians for bone augmentation procedures in preparation for implant dentistry. The aim of this study was to screen candidate HA biomaterials intended for alveolar ridge augmentation relative to their potential to support local bone formation/maturation and to assess biomaterial resorption using a routine critical-size rat calvaria defect model. Methods: Eighty adult male Sprague Dawley outbred rats obtained from a approved-breeder, randomized into groups of ten, were used. The calvaria defects (ø8 mm) either received sham surgery (empty control), Bio-Oss (bovine HA/reference control), or candidate biomaterials including bovine HA (Cerabone, DirectOss, 403Z013), and bovine (403Z014) or synthetic HA/ß-TCP (Reprobone, Ceraball) constructs. An 8 wk healing interval was used to capture the biomaterials’ resolution. Results: All biomaterials displayed biocompatibility. Strict HA biomaterials showed limited, if any, signs of biodegradation/resorption, with the biomaterial area fraction ranging from 22% to 42%. Synthetic HA/ß-TCP constructs showed limited evidence of biodegradation/erosion (biomaterial area fraction ≈30%). Mean linear defect closure in the sham-surgery control approximated 40%. Mean linear defect closure for the Bio-Oss reference control approximated 18% compared with 15–35% for the candidate biomaterials without significant differences between the controls and candidate biomaterials. Conclusions: None of the candidate HA biomaterials supported local bone formation/maturation beyond the native regenerative potential of this rodent model, pointing to their limitations for regenerative procedures. Biocompatibility and biomaterial dimensional stability could suggest their potential utility as long-term defect fillers. |
| first_indexed | 2024-03-09T18:27:33Z |
| format | Article |
| id | doaj.art-9230c2b7a56e4eec9126c3207243dbad |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T18:27:33Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9230c2b7a56e4eec9126c3207243dbad2023-11-24T07:47:38ZengMDPI AGBiomolecules2218-273X2022-11-011211167710.3390/biom12111677Screening of Hydroxyapatite Biomaterials for Alveolar Augmentation Using a Rat Calvaria Critical-Size Defect Model: Bone Formation/Maturation and Biomaterials ResolutionCristiano Susin0Jaebum Lee1Tiago Fiorini2Ki-Tae Koo3Peter Schüpbach4Amanda Finger Stadler5Ulf ME Wikesjö6Laboratory for Applied Periodontal & Craniofacial Research (LAPCR), Division of Comprehensive Oral Health, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USALaboratory for Applied Periodontal & Craniofacial Research (LAPCR), Division of Comprehensive Oral Health, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USALaboratory for Applied Periodontal & Craniofacial Research (LAPCR), Division of Comprehensive Oral Health, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USADepartment of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 110-460, KoreaSchüpbach Ltd., 8800 Thalwil, SwitzerlandLaboratory for Applied Periodontal & Craniofacial Research (LAPCR), Division of Comprehensive Oral Health, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USALaboratory for Applied Periodontal & Craniofacial Research (LAPCR), Division of Comprehensive Oral Health, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USABackground: Natural (bovine-/equine-/porcine-derived) or synthetic hydroxyapatite (HA) biomaterials appear to be the preferred technologies among clinicians for bone augmentation procedures in preparation for implant dentistry. The aim of this study was to screen candidate HA biomaterials intended for alveolar ridge augmentation relative to their potential to support local bone formation/maturation and to assess biomaterial resorption using a routine critical-size rat calvaria defect model. Methods: Eighty adult male Sprague Dawley outbred rats obtained from a approved-breeder, randomized into groups of ten, were used. The calvaria defects (ø8 mm) either received sham surgery (empty control), Bio-Oss (bovine HA/reference control), or candidate biomaterials including bovine HA (Cerabone, DirectOss, 403Z013), and bovine (403Z014) or synthetic HA/ß-TCP (Reprobone, Ceraball) constructs. An 8 wk healing interval was used to capture the biomaterials’ resolution. Results: All biomaterials displayed biocompatibility. Strict HA biomaterials showed limited, if any, signs of biodegradation/resorption, with the biomaterial area fraction ranging from 22% to 42%. Synthetic HA/ß-TCP constructs showed limited evidence of biodegradation/erosion (biomaterial area fraction ≈30%). Mean linear defect closure in the sham-surgery control approximated 40%. Mean linear defect closure for the Bio-Oss reference control approximated 18% compared with 15–35% for the candidate biomaterials without significant differences between the controls and candidate biomaterials. Conclusions: None of the candidate HA biomaterials supported local bone formation/maturation beyond the native regenerative potential of this rodent model, pointing to their limitations for regenerative procedures. Biocompatibility and biomaterial dimensional stability could suggest their potential utility as long-term defect fillers.https://www.mdpi.com/2218-273X/12/11/1677biocompatible materialsbonealveolar bone grafting |
| spellingShingle | Cristiano Susin Jaebum Lee Tiago Fiorini Ki-Tae Koo Peter Schüpbach Amanda Finger Stadler Ulf ME Wikesjö Screening of Hydroxyapatite Biomaterials for Alveolar Augmentation Using a Rat Calvaria Critical-Size Defect Model: Bone Formation/Maturation and Biomaterials Resolution Biomolecules biocompatible materials bone alveolar bone grafting |
| title | Screening of Hydroxyapatite Biomaterials for Alveolar Augmentation Using a Rat Calvaria Critical-Size Defect Model: Bone Formation/Maturation and Biomaterials Resolution |
| title_full | Screening of Hydroxyapatite Biomaterials for Alveolar Augmentation Using a Rat Calvaria Critical-Size Defect Model: Bone Formation/Maturation and Biomaterials Resolution |
| title_fullStr | Screening of Hydroxyapatite Biomaterials for Alveolar Augmentation Using a Rat Calvaria Critical-Size Defect Model: Bone Formation/Maturation and Biomaterials Resolution |
| title_full_unstemmed | Screening of Hydroxyapatite Biomaterials for Alveolar Augmentation Using a Rat Calvaria Critical-Size Defect Model: Bone Formation/Maturation and Biomaterials Resolution |
| title_short | Screening of Hydroxyapatite Biomaterials for Alveolar Augmentation Using a Rat Calvaria Critical-Size Defect Model: Bone Formation/Maturation and Biomaterials Resolution |
| title_sort | screening of hydroxyapatite biomaterials for alveolar augmentation using a rat calvaria critical size defect model bone formation maturation and biomaterials resolution |
| topic | biocompatible materials bone alveolar bone grafting |
| url | https://www.mdpi.com/2218-273X/12/11/1677 |
| work_keys_str_mv | AT cristianosusin screeningofhydroxyapatitebiomaterialsforalveolaraugmentationusingaratcalvariacriticalsizedefectmodelboneformationmaturationandbiomaterialsresolution AT jaebumlee screeningofhydroxyapatitebiomaterialsforalveolaraugmentationusingaratcalvariacriticalsizedefectmodelboneformationmaturationandbiomaterialsresolution AT tiagofiorini screeningofhydroxyapatitebiomaterialsforalveolaraugmentationusingaratcalvariacriticalsizedefectmodelboneformationmaturationandbiomaterialsresolution AT kitaekoo screeningofhydroxyapatitebiomaterialsforalveolaraugmentationusingaratcalvariacriticalsizedefectmodelboneformationmaturationandbiomaterialsresolution AT peterschupbach screeningofhydroxyapatitebiomaterialsforalveolaraugmentationusingaratcalvariacriticalsizedefectmodelboneformationmaturationandbiomaterialsresolution AT amandafingerstadler screeningofhydroxyapatitebiomaterialsforalveolaraugmentationusingaratcalvariacriticalsizedefectmodelboneformationmaturationandbiomaterialsresolution AT ulfmewikesjo screeningofhydroxyapatitebiomaterialsforalveolaraugmentationusingaratcalvariacriticalsizedefectmodelboneformationmaturationandbiomaterialsresolution |