Comparison of methods to recover amaranth weed seeds from manure
Abstract One pathway by which Palmer amaranth (Amaranthus palmeri S. Watson) invades new areas is through importation of contaminated livestock feed, which then contaminates land‐applied manure. If contaminated feed is suspected, detection tools are needed to test manure, but traditional methods are...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
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| Series: | Agricultural & Environmental Letters |
| Online Access: | https://doi.org/10.1002/ael2.20065 |
| _version_ | 1811245792958087168 |
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| author | Melissa L. Wilson Anthony Brusa Hatley Christensen Samuel Strack Eddie Alto Luis F. Allen Scott D. Cortus Chryseis Modderman Roger L. Becker |
| author_facet | Melissa L. Wilson Anthony Brusa Hatley Christensen Samuel Strack Eddie Alto Luis F. Allen Scott D. Cortus Chryseis Modderman Roger L. Becker |
| author_sort | Melissa L. Wilson |
| collection | DOAJ |
| description | Abstract One pathway by which Palmer amaranth (Amaranthus palmeri S. Watson) invades new areas is through importation of contaminated livestock feed, which then contaminates land‐applied manure. If contaminated feed is suspected, detection tools are needed to test manure, but traditional methods are time consuming and often inconclusive. Although new genetic seed testing is making detection easier, methods to separate seed from contaminated manure are needed. Six methods were compared for their ability to recover 100 Palmer amaranth seeds added to bedded or nonbedded cattle manure: dry sieving, rinse sieving, manure saturation sieving without blending and with blending, and dispersion sieving without blending and with blending. Seed recovery was highest (>90%) with the rinse sieving method regardless of manure type. The dispersion methods are not recommended as they recovered <24.7% of seeds. Following each method, genetic testing successfully identified Palmer amaranth presence, indicating no interference of recovery method with DNA extraction. |
| first_indexed | 2024-04-12T14:44:28Z |
| format | Article |
| id | doaj.art-92314a90ba564a2a8c321879d2986473 |
| institution | Directory Open Access Journal |
| issn | 2471-9625 |
| language | English |
| last_indexed | 2024-04-12T14:44:28Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Agricultural & Environmental Letters |
| spelling | doaj.art-92314a90ba564a2a8c321879d29864732022-12-22T03:28:42ZengWileyAgricultural & Environmental Letters2471-96252022-01-0171n/an/a10.1002/ael2.20065Comparison of methods to recover amaranth weed seeds from manureMelissa L. Wilson0Anthony Brusa1Hatley Christensen2Samuel Strack3Eddie Alto4Luis F. Allen5Scott D. Cortus6Chryseis Modderman7Roger L. Becker8Dep. of Soil, Water, and Climate Univ. of Minnesota 1991 Upper Buford Circle Saint Paul MN 55108 USADep. of Agronomy and Plant Genetics Univ. of Minnesota 1991 Upper Buford Circle Saint Paul MN 55108 USADep. of Soil, Water, and Climate Univ. of Minnesota 1991 Upper Buford Circle Saint Paul MN 55108 USADep. of Soil, Water, and Climate Univ. of Minnesota 1991 Upper Buford Circle Saint Paul MN 55108 USADep. of Soil, Water, and Climate Univ. of Minnesota 1991 Upper Buford Circle Saint Paul MN 55108 USADep. of Soil, Water, and Climate Univ. of Minnesota 1991 Upper Buford Circle Saint Paul MN 55108 USADep. of Soil, Water, and Climate Univ. of Minnesota 1991 Upper Buford Circle Saint Paul MN 55108 USAWest Central Research and Outreach Center Univ. of Minnesota Extension 46352 MN‐329 Morris MN 56267 USADep. of Agronomy and Plant Genetics Univ. of Minnesota 1991 Upper Buford Circle Saint Paul MN 55108 USAAbstract One pathway by which Palmer amaranth (Amaranthus palmeri S. Watson) invades new areas is through importation of contaminated livestock feed, which then contaminates land‐applied manure. If contaminated feed is suspected, detection tools are needed to test manure, but traditional methods are time consuming and often inconclusive. Although new genetic seed testing is making detection easier, methods to separate seed from contaminated manure are needed. Six methods were compared for their ability to recover 100 Palmer amaranth seeds added to bedded or nonbedded cattle manure: dry sieving, rinse sieving, manure saturation sieving without blending and with blending, and dispersion sieving without blending and with blending. Seed recovery was highest (>90%) with the rinse sieving method regardless of manure type. The dispersion methods are not recommended as they recovered <24.7% of seeds. Following each method, genetic testing successfully identified Palmer amaranth presence, indicating no interference of recovery method with DNA extraction.https://doi.org/10.1002/ael2.20065 |
| spellingShingle | Melissa L. Wilson Anthony Brusa Hatley Christensen Samuel Strack Eddie Alto Luis F. Allen Scott D. Cortus Chryseis Modderman Roger L. Becker Comparison of methods to recover amaranth weed seeds from manure Agricultural & Environmental Letters |
| title | Comparison of methods to recover amaranth weed seeds from manure |
| title_full | Comparison of methods to recover amaranth weed seeds from manure |
| title_fullStr | Comparison of methods to recover amaranth weed seeds from manure |
| title_full_unstemmed | Comparison of methods to recover amaranth weed seeds from manure |
| title_short | Comparison of methods to recover amaranth weed seeds from manure |
| title_sort | comparison of methods to recover amaranth weed seeds from manure |
| url | https://doi.org/10.1002/ael2.20065 |
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