Shrimp Lipid Prevents Endoplasmic Reticulum-Mediated Endothelial Cell Damage
Shrimp contains a fat that benefits cardiovascular function and may help in the prevention of diseases. The stress of essential cellular organelle endoplasmic reticulum (ER) is linked to endothelial dysfunction and damage. This research aimed at investigating the effect of shrimp lipid (SL) on endot...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/11/19/3076 |
| _version_ | 1797479322904166400 |
|---|---|
| author | Zin Zin Ei Soottawat Benjakul Natchaphol Buamard Kittichate Visuttijai Pithi Chanvorachote |
| author_facet | Zin Zin Ei Soottawat Benjakul Natchaphol Buamard Kittichate Visuttijai Pithi Chanvorachote |
| author_sort | Zin Zin Ei |
| collection | DOAJ |
| description | Shrimp contains a fat that benefits cardiovascular function and may help in the prevention of diseases. The stress of essential cellular organelle endoplasmic reticulum (ER) is linked to endothelial dysfunction and damage. This research aimed at investigating the effect of shrimp lipid (SL) on endothelial cells in response to ER stress, as well as the underlying mechanisms. Human endothelial cells were pretreated with SL (250 and 500 μg/mL) for 24 h, and treated with 0.16 μg/mL of Thapsigargin (Tg) for 24 h. The apoptosis and necrosis were detected by Hoechst 33342/propidium iodide (PI) co-staining. Cellular signaling pathways and ER stress markers were evaluated by Western blot analysis and immunofluorescence. SL protected against ER-induced endothelial cell apoptosis. According to the results, the viability of EA.hy926 cells treated with Tg alone was 44.97 ± 1%, but SL (250 μg/mL) pretreatment increased cell viability to 77.26 ± 3.9%, and SL (500 μg/mL) increased to 72.42 ± 4.3%. SL suppressed the increase in ER stress regulator glucose-regulated protein 78 (GRP78) and attenuated the RNA-dependent protein kinase-like ER eukaryotic initiation factor-2α kinase (PERK) and inositol-requiring ER-to-nucleus signaling protein 1 (IRE1) pathways. SL could inhibit cell damage by reducing the ER-related apoptosis protein, C/EBPα-homologous protein (CHOP), induced by ER stress. Taken together, we found the protective effect and mechanism of SL in protecting ER stress-induced endothelial cell apoptosis through suppression of the ER stress pathway. The findings may support the potential use of SL as an approach with a protective effect on endothelial cells. |
| first_indexed | 2024-03-09T21:44:12Z |
| format | Article |
| id | doaj.art-9231fbf2c06040938cf1b148c6432e1e |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T21:44:12Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-9231fbf2c06040938cf1b148c6432e1e2023-11-23T20:21:43ZengMDPI AGFoods2304-81582022-10-011119307610.3390/foods11193076Shrimp Lipid Prevents Endoplasmic Reticulum-Mediated Endothelial Cell DamageZin Zin Ei0Soottawat Benjakul1Natchaphol Buamard2Kittichate Visuttijai3Pithi Chanvorachote4Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, ThailandInternational Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90110, ThailandInternational Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Laboratory Medicine, Institute of Biomedicine, University of Gothenburg, 405 30 Gothenburg, SwedenDepartment of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, ThailandShrimp contains a fat that benefits cardiovascular function and may help in the prevention of diseases. The stress of essential cellular organelle endoplasmic reticulum (ER) is linked to endothelial dysfunction and damage. This research aimed at investigating the effect of shrimp lipid (SL) on endothelial cells in response to ER stress, as well as the underlying mechanisms. Human endothelial cells were pretreated with SL (250 and 500 μg/mL) for 24 h, and treated with 0.16 μg/mL of Thapsigargin (Tg) for 24 h. The apoptosis and necrosis were detected by Hoechst 33342/propidium iodide (PI) co-staining. Cellular signaling pathways and ER stress markers were evaluated by Western blot analysis and immunofluorescence. SL protected against ER-induced endothelial cell apoptosis. According to the results, the viability of EA.hy926 cells treated with Tg alone was 44.97 ± 1%, but SL (250 μg/mL) pretreatment increased cell viability to 77.26 ± 3.9%, and SL (500 μg/mL) increased to 72.42 ± 4.3%. SL suppressed the increase in ER stress regulator glucose-regulated protein 78 (GRP78) and attenuated the RNA-dependent protein kinase-like ER eukaryotic initiation factor-2α kinase (PERK) and inositol-requiring ER-to-nucleus signaling protein 1 (IRE1) pathways. SL could inhibit cell damage by reducing the ER-related apoptosis protein, C/EBPα-homologous protein (CHOP), induced by ER stress. Taken together, we found the protective effect and mechanism of SL in protecting ER stress-induced endothelial cell apoptosis through suppression of the ER stress pathway. The findings may support the potential use of SL as an approach with a protective effect on endothelial cells.https://www.mdpi.com/2304-8158/11/19/3076shrimp lipidGRP78CHOPER stressendothelial cells |
| spellingShingle | Zin Zin Ei Soottawat Benjakul Natchaphol Buamard Kittichate Visuttijai Pithi Chanvorachote Shrimp Lipid Prevents Endoplasmic Reticulum-Mediated Endothelial Cell Damage Foods shrimp lipid GRP78 CHOP ER stress endothelial cells |
| title | Shrimp Lipid Prevents Endoplasmic Reticulum-Mediated Endothelial Cell Damage |
| title_full | Shrimp Lipid Prevents Endoplasmic Reticulum-Mediated Endothelial Cell Damage |
| title_fullStr | Shrimp Lipid Prevents Endoplasmic Reticulum-Mediated Endothelial Cell Damage |
| title_full_unstemmed | Shrimp Lipid Prevents Endoplasmic Reticulum-Mediated Endothelial Cell Damage |
| title_short | Shrimp Lipid Prevents Endoplasmic Reticulum-Mediated Endothelial Cell Damage |
| title_sort | shrimp lipid prevents endoplasmic reticulum mediated endothelial cell damage |
| topic | shrimp lipid GRP78 CHOP ER stress endothelial cells |
| url | https://www.mdpi.com/2304-8158/11/19/3076 |
| work_keys_str_mv | AT zinzinei shrimplipidpreventsendoplasmicreticulummediatedendothelialcelldamage AT soottawatbenjakul shrimplipidpreventsendoplasmicreticulummediatedendothelialcelldamage AT natchapholbuamard shrimplipidpreventsendoplasmicreticulummediatedendothelialcelldamage AT kittichatevisuttijai shrimplipidpreventsendoplasmicreticulummediatedendothelialcelldamage AT pithichanvorachote shrimplipidpreventsendoplasmicreticulummediatedendothelialcelldamage |