CRISPy-web: An online resource to design sgRNAs for CRISPR applications

CRISPy-web: An online resource to design sgRNAs for CRISPR applications

CRISPR/Cas9-based genome editing has been one of the major achievements of molecular biology, allowing the targeted engineering of a wide range of genomes. The system originally evolved in prokaryotes as an adaptive immune system against bacteriophage infections. It now sees widespread application i...

Full description

Bibliographic Details
Main Authors:	Kai Blin, Lasse Ebdrup Pedersen, Tilmann Weber, Sang Yup Lee
Format:	Article
Language:	English
Published:	KeAi Communications Co., Ltd. 2016-06-01
Series:	Synthetic and Systems Biotechnology
Subjects:	CRISPR Cas9 sgRNA Guide RNA Genome editing Genome engineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2405805X15300168

Similar Items

Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0
by: Kai Blin, et al.
Published: (2020-06-01)

Mismatch Intolerance of 5′-Truncated sgRNAs in CRISPR/Cas9 Enables Efficient Microbial Single-Base Genome Editing
by: Ho Joung Lee, et al.
Published: (2021-06-01)

A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction
by: Yan Liang, et al.
Published: (2019-05-01)

CRISPR-surfaceome: An online tool for designing highly efficient sgRNAs targeting cell surface proteins
by: Hong Mei, et al.
Published: (2022-01-01)

Evaluating the cleavage efficacy of CRISPR-Cas9 sgRNAs targeting ineffective regions of Arabidopsis thaliana genome
by: Afsheen Malik, et al.
Published: (2021-05-01)

Effects of sgRNA length and number on gene editing efficiency and predicted mutations generated in rice
by: Xiaojing Liu, et al.
Published: (2022-04-01)

Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions
by: Joseph Blayney, et al.
Published: (2020-07-01)

Nanovesicle-Mediated Delivery Systems for CRISPR/Cas Genome Editing
by: Dongyoon Kim, et al.
Published: (2020-12-01)

Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
by: Sojung Kim, et al.
Published: (2017-11-01)

The CRISPR/Cas genome-editing tool: application in improvement of crops
by: SURENDER eKHATODIA, et al.
Published: (2016-04-01)

RETRACTED: Optimizing sgRNA to Improve CRISPR/Cas9 Knockout Efficiency: Special Focus on Human and Animal Cell
by: Sadegh Shojaei Baghini, et al.
Published: (2021-11-01)

AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design
by: Kathleen C. Keough, et al.
Published: (2019-08-01)

Computational Tools and Resources Supporting CRISPR-Cas Experiments
by: Pawel Sledzinski, et al.
Published: (2020-05-01)

Editing Citrus Genome via SaCas9/sgRNA System
by: Hongge Jia, et al.
Published: (2017-12-01)

Development and design of CRISPR-based diagnostic for Acinetobacter baumannii by employing off-target gene editing of sgRNA
by: Zulqarnain Baqar, et al.
Published: (2024-01-01)

CRISpy-Pop: A Web Tool for Designing CRISPR/Cas9-Driven Genetic Modifications in Diverse Populations
by: Hayley R. Stoneman, et al.
Published: (2020-11-01)

Computational Tools and Resources for CRISPR/Cas Genome Editing
by: Chao Li, et al.
Published: (2023-02-01)

Factors affecting the cleavage efficiency of the CRISPR-Cas9 system
by: Won Jun Jung, et al.
Published: (2024-12-01)

Machine learning in the estimation of CRISPR-Cas9 cleavage sites for plant system
by: Jutan Das, et al.
Published: (2023-01-01)

CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects
by: Hakim Manghwar, et al.
Published: (2020-03-01)

Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton
by: Mohamed Ramadan, et al.
Published: (2021-02-01)

sgRNA-shRNA Structure Mediated SNP Site Editing on Porcine IGF2 Gene by CRISPR/StCas9
by: Yongsen Sun, et al.
Published: (2019-04-01)

CNN-XG: A Hybrid Framework for sgRNA On-Target Prediction
by: Bohao Li, et al.
Published: (2022-03-01)

Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis
by: Jing Li, et al.
Published: (2024-01-01)

Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding
by: Junming Zhou, et al.
Published: (2023-03-01)

Improving Stability and Specificity of CRISPR/Cas9 System by Selective Modification of Guide RNAs with 2′-fluoro and Locked Nucleic Acid Nucleotides
by: Lubov Sakovina, et al.
Published: (2022-11-01)

The efficient generation of knockout microglia cells using a dual-sgRNA strategy by CRISPR/Cas9
by: Mengfei Zhang, et al.
Published: (2022-10-01)

CRISPRpred(SEQ): a sequence-based method for sgRNA on target activity prediction using traditional machine learning
by: Ali Haisam Muhammad Rafid, et al.
Published: (2020-06-01)

Genome Engineering in Plant Using an Efficient CRISPR-xCas9 Toolset With an Expanded PAM Compatibility
by: Chengwei Zhang, et al.
Published: (2020-12-01)

Effects of sgRNAs, Promoters, and Explants on the Gene Editing Efficiency of the CRISPR/Cas9 System in Chinese Kale
by: Wenli Huang, et al.
Published: (2023-08-01)

A consolidated AAV system for single-cut CRISPR correction of a common Duchenne muscular dystrophy mutation
by: Yu Zhang, et al.
Published: (2021-09-01)

Identification of early flowering mutant gene in Phalaenopsis amabilis (L.) Blume for sgRNA construction in CRISPR/Cas9 genome editing system
by: N. P. A. E. O. Suputri, et al.
Published: (2023-06-01)

Mutation of <i>GmIPK1</i> Gene Using CRISPR/Cas9 Reduced Phytic Acid Content in Soybean Seeds
by: Ji Hyeon Song, et al.
Published: (2022-09-01)

Construction of EZH2 Knockout Animal Model by CRISPR/Cas9 Technology
by: Fanrong MENG, et al.
Published: (2018-05-01)

A systematic mapping study on machine learning techniques for the prediction of CRISPR/Cas9 sgRNA target cleavage
by: Giovanni Dimauro, et al.
Published: (2022-01-01)

CriSNPr, a single interface for the curated and de novo design of gRNAs for CRISPR diagnostics using diverse Cas systems
by: Asgar H Ansari, et al.
Published: (2023-02-01)

Heritable gene editing using FT mobile guide RNAs and DNA viruses
by: Jianfeng Lei, et al.
Published: (2021-02-01)

Off-target effects in CRISPR/Cas9 gene editing
by: Congting Guo, et al.
Published: (2023-03-01)

A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish
by: Sergey V. Prykhozhij, et al.
Published: (2017-06-01)

An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production
by: Jiaxin Zhang, et al.
Published: (2024-02-01)