Biological Activities of Molecules Derived from <i>Olea europaea</i> L. Tested <i>In Vitro</i>
Background: Extra virgin olive oil is a typical food of the Mediterranean area, obtained by pressing <i>Olea europaea</i> L. fruits. Its polyphenols have been studied for their antioxidant function and protective action against cancer and chronic kidney disease. In this <i>in vitro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/14/1/49 |
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| author | Giulia Marrone Silvia Urciuoli Eleonora Candi Roberta Bernini Gianluca Vanni Claudia Masci Cristina Guerriero Mara Mancini Antonino De Lorenzo Pamela Vignolini Annalisa Noce |
| author_facet | Giulia Marrone Silvia Urciuoli Eleonora Candi Roberta Bernini Gianluca Vanni Claudia Masci Cristina Guerriero Mara Mancini Antonino De Lorenzo Pamela Vignolini Annalisa Noce |
| author_sort | Giulia Marrone |
| collection | DOAJ |
| description | Background: Extra virgin olive oil is a typical food of the Mediterranean area, obtained by pressing <i>Olea europaea</i> L. fruits. Its polyphenols have been studied for their antioxidant function and protective action against cancer and chronic kidney disease. In this <i>in vitro</i> study, we tested titrated extracts from <i>Olea europaea</i> L. on a human embryonic kidney 293 (HEK-293E) cell line, regarding their pro-apoptotic and antiproliferative capacities, using “ IncuCyte<sup>®</sup> S3 Live-Cell Analysis System”. Materials and Methods: We selected <i>Olea europaea</i> L. active compounds like hydroxytyrosol (HT) and oleuropein (OLE). These extracts were tested at different concentrations and characterized by HPLC-DAD-MS for the content in secondary active metabolites. The real-time observation of cell behavior was performed by IncuCyte, which can quantitatively analyze the cell proliferation and death. Results: This study showed that all the tested extracts can significantly inhibit cellular growth at 50 µM but the reduced proliferation is not related to an increase in cellular apoptosis. Instead, the same analysis performed by using extracts at 100 µM reveals that they can inhibit cellular growth, further inducing cellular apoptosis. Conclusions: The results on the HEK-293E cells confirmed the antiproliferative and proapoptotic actions of active compounds from an <i>Olea europaea</i> L. matrix in this cell line. |
| first_indexed | 2024-03-08T10:44:30Z |
| format | Article |
| id | doaj.art-923357bab65645eea21fc24c307c7bb3 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-08T10:44:30Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-923357bab65645eea21fc24c307c7bb32024-01-26T17:20:34ZengMDPI AGLife2075-17292023-12-011414910.3390/life14010049Biological Activities of Molecules Derived from <i>Olea europaea</i> L. Tested <i>In Vitro</i>Giulia Marrone0Silvia Urciuoli1Eleonora Candi2Roberta Bernini3Gianluca Vanni4Claudia Masci5Cristina Guerriero6Mara Mancini7Antonino De Lorenzo8Pamela Vignolini9Annalisa Noce10Department of Systems Medicine, University of Rome Tor Vergata, 00133 Rome, ItalyPHYTOLAB (Pharmaceutical, Cosmetic, Food Supplement, Technology and Analysis)—DiSIA, University of Florence, 50019 Florence, ItalyDepartment of Experimental Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyDepartment of Agriculture and Forest Sciences (DAFNE), University of Tuscia, Via San Camillo de Lellis, 01100 Viterbo, ItalyBreast Unit, Department of Surgical Science, Policlinico Tor Vergata University, 00133 Rome, ItalyDepartment of Systems Medicine, University of Rome Tor Vergata, 00133 Rome, ItalyDepartment of Systems Medicine, University of Rome Tor Vergata, 00133 Rome, ItalyIstituto Dermatopatico Dell’Immacolata—IDI, Istituto di Ricovero e Cura a Carattere Scientifico—IRCCS, Via Monti di Creta 104, 00166 Rome, ItalySection of Clinical Nutrition and Nutrigenomic, Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, ItalyPHYTOLAB (Pharmaceutical, Cosmetic, Food Supplement, Technology and Analysis)—DiSIA, University of Florence, 50019 Florence, ItalyDepartment of Systems Medicine, University of Rome Tor Vergata, 00133 Rome, ItalyBackground: Extra virgin olive oil is a typical food of the Mediterranean area, obtained by pressing <i>Olea europaea</i> L. fruits. Its polyphenols have been studied for their antioxidant function and protective action against cancer and chronic kidney disease. In this <i>in vitro</i> study, we tested titrated extracts from <i>Olea europaea</i> L. on a human embryonic kidney 293 (HEK-293E) cell line, regarding their pro-apoptotic and antiproliferative capacities, using “ IncuCyte<sup>®</sup> S3 Live-Cell Analysis System”. Materials and Methods: We selected <i>Olea europaea</i> L. active compounds like hydroxytyrosol (HT) and oleuropein (OLE). These extracts were tested at different concentrations and characterized by HPLC-DAD-MS for the content in secondary active metabolites. The real-time observation of cell behavior was performed by IncuCyte, which can quantitatively analyze the cell proliferation and death. Results: This study showed that all the tested extracts can significantly inhibit cellular growth at 50 µM but the reduced proliferation is not related to an increase in cellular apoptosis. Instead, the same analysis performed by using extracts at 100 µM reveals that they can inhibit cellular growth, further inducing cellular apoptosis. Conclusions: The results on the HEK-293E cells confirmed the antiproliferative and proapoptotic actions of active compounds from an <i>Olea europaea</i> L. matrix in this cell line.https://www.mdpi.com/2075-1729/14/1/49<i>Olea europaea</i> L.Mediterranean diethydroxytyrosololeuropeinchronic degenerative non-communicable diseasesHEK-293E |
| spellingShingle | Giulia Marrone Silvia Urciuoli Eleonora Candi Roberta Bernini Gianluca Vanni Claudia Masci Cristina Guerriero Mara Mancini Antonino De Lorenzo Pamela Vignolini Annalisa Noce Biological Activities of Molecules Derived from <i>Olea europaea</i> L. Tested <i>In Vitro</i> Life <i>Olea europaea</i> L. Mediterranean diet hydroxytyrosol oleuropein chronic degenerative non-communicable diseases HEK-293E |
| title | Biological Activities of Molecules Derived from <i>Olea europaea</i> L. Tested <i>In Vitro</i> |
| title_full | Biological Activities of Molecules Derived from <i>Olea europaea</i> L. Tested <i>In Vitro</i> |
| title_fullStr | Biological Activities of Molecules Derived from <i>Olea europaea</i> L. Tested <i>In Vitro</i> |
| title_full_unstemmed | Biological Activities of Molecules Derived from <i>Olea europaea</i> L. Tested <i>In Vitro</i> |
| title_short | Biological Activities of Molecules Derived from <i>Olea europaea</i> L. Tested <i>In Vitro</i> |
| title_sort | biological activities of molecules derived from i olea europaea i l tested i in vitro i |
| topic | <i>Olea europaea</i> L. Mediterranean diet hydroxytyrosol oleuropein chronic degenerative non-communicable diseases HEK-293E |
| url | https://www.mdpi.com/2075-1729/14/1/49 |
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