Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus
RNA viruses, such as foot-and-mouth disease virus (FMDV), have error-prone replication resulting in the continuous emergence of new viral strains capable of evading current vaccine coverage. Vaccine formulations must be regularly updated, which is both costly and technically challenging for many vac...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/14/5/989 |
| _version_ | 1797494594849472512 |
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| author | Michael Puckette Victoria Primavera Erica Martel Jose Barrera William Hurtle Benjamin Clark Barbara Kamicker Mariceny Zurita David Brake John Neilan |
| author_facet | Michael Puckette Victoria Primavera Erica Martel Jose Barrera William Hurtle Benjamin Clark Barbara Kamicker Mariceny Zurita David Brake John Neilan |
| author_sort | Michael Puckette |
| collection | DOAJ |
| description | RNA viruses, such as foot-and-mouth disease virus (FMDV), have error-prone replication resulting in the continuous emergence of new viral strains capable of evading current vaccine coverage. Vaccine formulations must be regularly updated, which is both costly and technically challenging for many vaccine platforms. In this report, we describe a plasmid-based virus-like particle (VLP) production platform utilizing transiently transfected mammalian cell cultures that combines both the rapid response adaptability of nucleic-acid-based vaccines with the ability to produce intact capsid epitopes required for immunity. Formulated vaccines which employed this platform conferred complete protection from clinical foot-and-mouth disease in both swine and cattle. This novel platform can be quickly adapted to new viral strains and serotypes through targeted exchanges of only the FMDV capsid polypeptide nucleic acid sequences, from which processed structural capsid proteins are derived. This platform obviates the need for high biocontainment manufacturing facilities to produce inactivated whole-virus vaccines from infected mammalian cell cultures, which requires upstream expansion and downstream concentration of large quantities of live virulent viruses. |
| first_indexed | 2024-03-10T01:36:35Z |
| format | Article |
| id | doaj.art-9239021b67c9409195de47fcc6c6c543 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T01:36:35Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-9239021b67c9409195de47fcc6c6c5432023-11-23T13:31:29ZengMDPI AGViruses1999-49152022-05-0114598910.3390/v14050989Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease VirusMichael Puckette0Victoria Primavera1Erica Martel2Jose Barrera3William Hurtle4Benjamin Clark5Barbara Kamicker6Mariceny Zurita7David Brake8John Neilan9Plum Island Animal Disease Center, U.S. Department of Homeland Security Science and Technology Directorate, New York, NY 11944, USAPlum Island Animal Disease Center, Leidos, New York, NY 11944, USAPlum Island Animal Disease Center Research Participation Program, Oak Ridge Institute for Science and Education, New York, NY 11944, USAPlum Island Animal Disease Center, Leidos, New York, NY 11944, USAPlum Island Animal Disease Center, U.S. Department of Homeland Security Science and Technology Directorate, New York, NY 11944, USAPlum Island Animal Disease Center Research Participation Program, Oak Ridge Institute for Science and Education, New York, NY 11944, USAPlum Island Animal Disease Center, Leidos, New York, NY 11944, USAPlum Island Animal Disease Center, Leidos, New York, NY 11944, USABioQuest Associates, LLC, P.O. Box 787, Stowe, VT 05672, USAPlum Island Animal Disease Center, U.S. Department of Homeland Security Science and Technology Directorate, New York, NY 11944, USARNA viruses, such as foot-and-mouth disease virus (FMDV), have error-prone replication resulting in the continuous emergence of new viral strains capable of evading current vaccine coverage. Vaccine formulations must be regularly updated, which is both costly and technically challenging for many vaccine platforms. In this report, we describe a plasmid-based virus-like particle (VLP) production platform utilizing transiently transfected mammalian cell cultures that combines both the rapid response adaptability of nucleic-acid-based vaccines with the ability to produce intact capsid epitopes required for immunity. Formulated vaccines which employed this platform conferred complete protection from clinical foot-and-mouth disease in both swine and cattle. This novel platform can be quickly adapted to new viral strains and serotypes through targeted exchanges of only the FMDV capsid polypeptide nucleic acid sequences, from which processed structural capsid proteins are derived. This platform obviates the need for high biocontainment manufacturing facilities to produce inactivated whole-virus vaccines from infected mammalian cell cultures, which requires upstream expansion and downstream concentration of large quantities of live virulent viruses.https://www.mdpi.com/1999-4915/14/5/989virus-like particlefoot-and-mouth diseasefoot-and-mouth disease virusvaccineswinecattle |
| spellingShingle | Michael Puckette Victoria Primavera Erica Martel Jose Barrera William Hurtle Benjamin Clark Barbara Kamicker Mariceny Zurita David Brake John Neilan Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus Viruses virus-like particle foot-and-mouth disease foot-and-mouth disease virus vaccine swine cattle |
| title | Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus |
| title_full | Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus |
| title_fullStr | Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus |
| title_full_unstemmed | Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus |
| title_short | Transiently Transfected Mammalian Cell Cultures: An Adaptable and Effective Platform for Virus-like Particle-Based Vaccines against Foot-and-Mouth Disease Virus |
| title_sort | transiently transfected mammalian cell cultures an adaptable and effective platform for virus like particle based vaccines against foot and mouth disease virus |
| topic | virus-like particle foot-and-mouth disease foot-and-mouth disease virus vaccine swine cattle |
| url | https://www.mdpi.com/1999-4915/14/5/989 |
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