Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato
Auxin response factors (ARFs) are a family of transcription factors that play an important role of auxin regulation through their binding with auxin response elements. ARF genes are represented by a large multigene family in plants; however, to our knowledge, the ARF gene family has not been well st...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/17/9391 |
| _version_ | 1797521319740309504 |
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| author | Isaac Seth Pratt Baohong Zhang |
| author_facet | Isaac Seth Pratt Baohong Zhang |
| author_sort | Isaac Seth Pratt |
| collection | DOAJ |
| description | Auxin response factors (ARFs) are a family of transcription factors that play an important role of auxin regulation through their binding with auxin response elements. ARF genes are represented by a large multigene family in plants; however, to our knowledge, the ARF gene family has not been well studied and characterized in sweet potatoes. In this study, a total of 25 ARF genes were identified in <i>Ipomea trifida</i>. The identified <i>ItrARF</i> genes’ conserved motifs, chromosomal locations, phylogenetic relationships, and their protein characteristics were systemically investigated using different bioinformatics tools. The expression patterns of ItfARF genes were analyzed within the storage roots and normal roots at an early stage of development. ItfARF16b and ItfARF16c were both highly expressed in the storage root, with minimal to no expression in the normal root. ItfARF6a and ItfARF10a exhibited higher expression in the normal root but not in the storage root. Subsequently, ItfARF1a, ItfARF2b, ItfARF3a, ItfARF6b, ItfARF8a, ItfARF8b, and ItfARF10b were expressed in both root types with moderate to high expression for each. All ten of these ARF genes and their prominent expression signify their importance within the development of each respective root type. This study provides comprehensive information regarding the ARF family in sweet potatoes, which will be useful for future research to discover further functional verification of these ItfARF genes. |
| first_indexed | 2024-03-10T08:10:56Z |
| format | Article |
| id | doaj.art-923bab5db35844ea82901c2cf67c8f9a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:10:56Z |
| publishDate | 2021-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-923bab5db35844ea82901c2cf67c8f9a2023-11-22T10:42:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012217939110.3390/ijms22179391Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet PotatoIsaac Seth Pratt0Baohong Zhang1Department of Biology, East Carolina University, Greenville, NC 27858, USADepartment of Biology, East Carolina University, Greenville, NC 27858, USAAuxin response factors (ARFs) are a family of transcription factors that play an important role of auxin regulation through their binding with auxin response elements. ARF genes are represented by a large multigene family in plants; however, to our knowledge, the ARF gene family has not been well studied and characterized in sweet potatoes. In this study, a total of 25 ARF genes were identified in <i>Ipomea trifida</i>. The identified <i>ItrARF</i> genes’ conserved motifs, chromosomal locations, phylogenetic relationships, and their protein characteristics were systemically investigated using different bioinformatics tools. The expression patterns of ItfARF genes were analyzed within the storage roots and normal roots at an early stage of development. ItfARF16b and ItfARF16c were both highly expressed in the storage root, with minimal to no expression in the normal root. ItfARF6a and ItfARF10a exhibited higher expression in the normal root but not in the storage root. Subsequently, ItfARF1a, ItfARF2b, ItfARF3a, ItfARF6b, ItfARF8a, ItfARF8b, and ItfARF10b were expressed in both root types with moderate to high expression for each. All ten of these ARF genes and their prominent expression signify their importance within the development of each respective root type. This study provides comprehensive information regarding the ARF family in sweet potatoes, which will be useful for future research to discover further functional verification of these ItfARF genes.https://www.mdpi.com/1422-0067/22/17/9391sweet potatoauxin response factorroot development |
| spellingShingle | Isaac Seth Pratt Baohong Zhang Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato International Journal of Molecular Sciences sweet potato auxin response factor root development |
| title | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
| title_full | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
| title_fullStr | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
| title_full_unstemmed | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
| title_short | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
| title_sort | genome wide identification of arf transcription factor gene family and their expression analysis in sweet potato |
| topic | sweet potato auxin response factor root development |
| url | https://www.mdpi.com/1422-0067/22/17/9391 |
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