Removal of Toxin (Tetrodotoxin) from Puffer Ovary by Traditional Fermentation
The amounts of puffer toxin (tetrodotoxin, TTX) extracted from the fresh and the traditional Japanese salted and fermented “Nukazuke” and “Kasuzuke” ovaries of Takifugu stictonotus (T. stictonotus) were quantitatively analyzed in the voltage-de...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-01-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/5/1/193 |
| _version_ | 1811301262908456960 |
|---|---|
| author | Norio Akaike Ayaka Hamamoto Masahito Wakita Min-Chul Shin Takatoshi Yamamoto Toshitaka Yamaga Kensaku Anraku Kiku Nonaka |
| author_facet | Norio Akaike Ayaka Hamamoto Masahito Wakita Min-Chul Shin Takatoshi Yamamoto Toshitaka Yamaga Kensaku Anraku Kiku Nonaka |
| author_sort | Norio Akaike |
| collection | DOAJ |
| description | The amounts of puffer toxin (tetrodotoxin, TTX) extracted from the fresh and the traditional Japanese salted and fermented “Nukazuke” and “Kasuzuke” ovaries of Takifugu stictonotus (T. stictonotus) were quantitatively analyzed in the voltage-dependent sodium current (INa) recorded from mechanically dissociated single rat hippocampal CA1 neurons. The amount of TTX contained in “Nukazuke” and “Kasuzuke” ovaries decreased to 1/50–1/90 times of that of fresh ovary during a salted and successive fermented period over a few years. The final toxin concentration after fermentation was almost close to the TTX level extracted from T. Rubripes” fresh muscle that is normally eaten. It was concluded that the fermented “Nukazuke” and “Kasuzuke” ovaries of puffer fish T. Stictonotus are safe and harmless as food. |
| first_indexed | 2024-04-13T07:05:39Z |
| format | Article |
| id | doaj.art-923f39bddac8495c8b5e113a6f4258a7 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-13T07:05:39Z |
| publishDate | 2013-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-923f39bddac8495c8b5e113a6f4258a72022-12-22T02:57:00ZengMDPI AGToxins2072-66512013-01-015119320210.3390/toxins5010193Removal of Toxin (Tetrodotoxin) from Puffer Ovary by Traditional FermentationNorio AkaikeAyaka HamamotoMasahito WakitaMin-Chul ShinTakatoshi YamamotoToshitaka YamagaKensaku AnrakuKiku NonakaThe amounts of puffer toxin (tetrodotoxin, TTX) extracted from the fresh and the traditional Japanese salted and fermented “Nukazuke” and “Kasuzuke” ovaries of Takifugu stictonotus (T. stictonotus) were quantitatively analyzed in the voltage-dependent sodium current (INa) recorded from mechanically dissociated single rat hippocampal CA1 neurons. The amount of TTX contained in “Nukazuke” and “Kasuzuke” ovaries decreased to 1/50–1/90 times of that of fresh ovary during a salted and successive fermented period over a few years. The final toxin concentration after fermentation was almost close to the TTX level extracted from T. Rubripes” fresh muscle that is normally eaten. It was concluded that the fermented “Nukazuke” and “Kasuzuke” ovaries of puffer fish T. Stictonotus are safe and harmless as food.http://www.mdpi.com/2072-6651/5/1/193puffer fishTakifugu Stictonotusfermented puffer&#8217s ovarytetrodotoxinrat hippocampal neuronsodium current |
| spellingShingle | Norio Akaike Ayaka Hamamoto Masahito Wakita Min-Chul Shin Takatoshi Yamamoto Toshitaka Yamaga Kensaku Anraku Kiku Nonaka Removal of Toxin (Tetrodotoxin) from Puffer Ovary by Traditional Fermentation Toxins puffer fish Takifugu Stictonotus fermented puffer&#8217 s ovary tetrodotoxin rat hippocampal neuron sodium current |
| title | Removal of Toxin (Tetrodotoxin) from Puffer Ovary by Traditional Fermentation |
| title_full | Removal of Toxin (Tetrodotoxin) from Puffer Ovary by Traditional Fermentation |
| title_fullStr | Removal of Toxin (Tetrodotoxin) from Puffer Ovary by Traditional Fermentation |
| title_full_unstemmed | Removal of Toxin (Tetrodotoxin) from Puffer Ovary by Traditional Fermentation |
| title_short | Removal of Toxin (Tetrodotoxin) from Puffer Ovary by Traditional Fermentation |
| title_sort | removal of toxin tetrodotoxin from puffer ovary by traditional fermentation |
| topic | puffer fish Takifugu Stictonotus fermented puffer&#8217 s ovary tetrodotoxin rat hippocampal neuron sodium current |
| url | http://www.mdpi.com/2072-6651/5/1/193 |
| work_keys_str_mv | AT norioakaike removaloftoxintetrodotoxinfrompufferovarybytraditionalfermentation AT ayakahamamoto removaloftoxintetrodotoxinfrompufferovarybytraditionalfermentation AT masahitowakita removaloftoxintetrodotoxinfrompufferovarybytraditionalfermentation AT minchulshin removaloftoxintetrodotoxinfrompufferovarybytraditionalfermentation AT takatoshiyamamoto removaloftoxintetrodotoxinfrompufferovarybytraditionalfermentation AT toshitakayamaga removaloftoxintetrodotoxinfrompufferovarybytraditionalfermentation AT kensakuanraku removaloftoxintetrodotoxinfrompufferovarybytraditionalfermentation AT kikunonaka removaloftoxintetrodotoxinfrompufferovarybytraditionalfermentation |