Evaluation of Myceliopthora thermophila as an Enzyme Factory for the Production of Thermophilic Cellulolytic Enzymes
Enzymatic hydrolysis is a key step in bioethanol production. Efficient hydrolysis requires a consortium of different enzymes that are able to hydrolyze cellulose and hemicellulose into fermentable sugars. Myceliopthora thermophila is a promising candidate for the production of thermophilic celluloly...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2015-07-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_5140_Matsakas_Evaluation_Myceliopthora_Enzyme_Factory |
| _version_ | 1818424915595886592 |
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| author | Leonidas Matsakas Io Antonopoulou Paul Christakopoulos |
| author_facet | Leonidas Matsakas Io Antonopoulou Paul Christakopoulos |
| author_sort | Leonidas Matsakas |
| collection | DOAJ |
| description | Enzymatic hydrolysis is a key step in bioethanol production. Efficient hydrolysis requires a consortium of different enzymes that are able to hydrolyze cellulose and hemicellulose into fermentable sugars. Myceliopthora thermophila is a promising candidate for the production of thermophilic cellulolytic enzymes, the use of which could reduce the cost of ethanol production. The growth conditions of the fungus were optimized in order to achieve increased secretion of extracellular cellulases. Optimal conditions were found to be 7.0% w/v brewer’s spent grain as the carbon source and 0.4% w/v ammonium sulfate as the nitrogen source. The cellulases obtained were characterized for their optimum activity. The optimum temperature and pH for cellulase activity are 65 °C and pH 5.5, respectively. Studies on thermal inactivation of the crude extract showed that the cellulases of M. thermophila are stable for temperatures up to 60 °C. At this temperature the half-life was found to be as high as 27 h. Enzymatic hydrolysis of cellulose resulted in 31.4% hydrolysis yield at 60 °C after 24 h of incubation. Finally, the recalcitrance constant for cellulose and cellulose pretreated with ionic liquids was calculated to be 5.46 and 2.69, respectively. |
| first_indexed | 2024-12-14T14:05:38Z |
| format | Article |
| id | doaj.art-923f5f1601c243cd80addea72bb726d3 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-14T14:05:38Z |
| publishDate | 2015-07-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-923f5f1601c243cd80addea72bb726d32022-12-21T22:58:33ZengNorth Carolina State UniversityBioResources1930-21261930-21262015-07-011035140515810.15376/biores.10.3.5140-5158Evaluation of Myceliopthora thermophila as an Enzyme Factory for the Production of Thermophilic Cellulolytic EnzymesLeonidas Matsakas0Io Antonopoulou1Paul Christakopoulos2Luleå University of Technology; SwedenLuleå University of Technology; SwedenLuleå University of Technology; SwedenEnzymatic hydrolysis is a key step in bioethanol production. Efficient hydrolysis requires a consortium of different enzymes that are able to hydrolyze cellulose and hemicellulose into fermentable sugars. Myceliopthora thermophila is a promising candidate for the production of thermophilic cellulolytic enzymes, the use of which could reduce the cost of ethanol production. The growth conditions of the fungus were optimized in order to achieve increased secretion of extracellular cellulases. Optimal conditions were found to be 7.0% w/v brewer’s spent grain as the carbon source and 0.4% w/v ammonium sulfate as the nitrogen source. The cellulases obtained were characterized for their optimum activity. The optimum temperature and pH for cellulase activity are 65 °C and pH 5.5, respectively. Studies on thermal inactivation of the crude extract showed that the cellulases of M. thermophila are stable for temperatures up to 60 °C. At this temperature the half-life was found to be as high as 27 h. Enzymatic hydrolysis of cellulose resulted in 31.4% hydrolysis yield at 60 °C after 24 h of incubation. Finally, the recalcitrance constant for cellulose and cellulose pretreated with ionic liquids was calculated to be 5.46 and 2.69, respectively.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_5140_Matsakas_Evaluation_Myceliopthora_Enzyme_FactoryEnzymatic hydrolysisBiofuelsBioethanolMyceliopthora thermophilaSporotrichum thermophileLignocellulosic biomassThermophilic enzymes |
| spellingShingle | Leonidas Matsakas Io Antonopoulou Paul Christakopoulos Evaluation of Myceliopthora thermophila as an Enzyme Factory for the Production of Thermophilic Cellulolytic Enzymes BioResources Enzymatic hydrolysis Biofuels Bioethanol Myceliopthora thermophila Sporotrichum thermophile Lignocellulosic biomass Thermophilic enzymes |
| title | Evaluation of Myceliopthora thermophila as an Enzyme Factory for the Production of Thermophilic Cellulolytic Enzymes |
| title_full | Evaluation of Myceliopthora thermophila as an Enzyme Factory for the Production of Thermophilic Cellulolytic Enzymes |
| title_fullStr | Evaluation of Myceliopthora thermophila as an Enzyme Factory for the Production of Thermophilic Cellulolytic Enzymes |
| title_full_unstemmed | Evaluation of Myceliopthora thermophila as an Enzyme Factory for the Production of Thermophilic Cellulolytic Enzymes |
| title_short | Evaluation of Myceliopthora thermophila as an Enzyme Factory for the Production of Thermophilic Cellulolytic Enzymes |
| title_sort | evaluation of myceliopthora thermophila as an enzyme factory for the production of thermophilic cellulolytic enzymes |
| topic | Enzymatic hydrolysis Biofuels Bioethanol Myceliopthora thermophila Sporotrichum thermophile Lignocellulosic biomass Thermophilic enzymes |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_3_5140_Matsakas_Evaluation_Myceliopthora_Enzyme_Factory |
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