Identification of Antioxidant Components and Tyrosinase Specific Inhibitors from Osmanthus fragrans Flower by Using Online UPLC-ABTS+·-assay and UF- LC-MS Technology
Objective: To investigate and analyze antioxidant activities and tyrosinase inhibition of the aqueous extract of Osmanthus fragrans flower (O. fragrans), as well as its active ingredients. Methods: In this study, antioxidant activities of extract of O. fragrans were carried out by using DPPH·, ABTS+...
| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-04-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070130 |
| _version_ | 1828093790883151872 |
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| author | Huiji ZHOU Tingzhao LI Bo LI |
| author_facet | Huiji ZHOU Tingzhao LI Bo LI |
| author_sort | Huiji ZHOU |
| collection | DOAJ |
| description | Objective: To investigate and analyze antioxidant activities and tyrosinase inhibition of the aqueous extract of Osmanthus fragrans flower (O. fragrans), as well as its active ingredients. Methods: In this study, antioxidant activities of extract of O. fragrans were carried out by using DPPH·, ABTS+· radical scavenging assays and total antioxidant activity (FRAP). Qualitative analysis of major active components was performed by UPLC-PDA-QDa-ABTS+· online method and UF-LC-MS method were used to qualitatively identify the antioxidant components and tyrosinase inhibitors from O. fragrans extract respectively. Oxidative damage model of skin fibroblasts induced by H2O2 was used to evaluate the protective effect of oxidative stress of O. fragrans aqueous extract and its chemical components. Results: Aqueous extract of O. fragrans had good scavenging effect on DPPH· and ABTS+· free radical in vitro, and its EC50 values of free radical scavenging effect on DPPH· and ABTS+· were 37.66, 38.32 μg/mL, respectively. When the concentration of O. fragrans extract reached 2 mg/mL, FRAP value could be as high as 3.17. The EC50 of tyrosinase diphenoloxidase inhibition of O. fragrans extract was 595.9 μg/mL. Through analysis of UPLC-Triple-TOF/MS, a total of 28 chemical compositions were preliminarily identified. Five antioxidant active compounds which also greatly combined with tyrosinase were screened out from O. fragrans extract by simultaneously using UPLC-PDA-QDa-ABTS+· online method and UF-LC-MS method. Among them, the main active ingredient was verbascoside. Antioxidant and tyrosinase assay showed that verbascoside had significant antioxidant activity and tyrosinase inhibitory activity. EC50 values of verbascoside of scavenging effect on DPPH· and ABTS+· were 10.27, 14.96 μg/mL, respectively. When the concentration of verbascoside was 1 mg/mL, FRAP value could be up to 4.22. The EC50 of tyrosinase monophenoloxidase inhibition of verbascoside was 477.5 μg/mL, but with little diphenoloxidase inhibitiory effect. The oxidative damage model induced by H2O2 was used to further verify the significant protective effects of O. fragrans extract and verbascoside on oxidative damage of skin fibroblasts. Conclusion: The aqueous extract of O. fragrans and its main active component, verbascoside, had good antioxidant activity, tyrosinase inhibitory activity and oxidative stress protection ability. |
| first_indexed | 2024-04-11T06:49:22Z |
| format | Article |
| id | doaj.art-92409054b2f74b34b1a6d76ba9758c44 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T06:49:22Z |
| publishDate | 2022-04-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-92409054b2f74b34b1a6d76ba9758c442022-12-22T04:39:15ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-04-01437677910.13386/j.issn1002-0306.20210701302021070130-7Identification of Antioxidant Components and Tyrosinase Specific Inhibitors from Osmanthus fragrans Flower by Using Online UPLC-ABTS+·-assay and UF- LC-MS TechnologyHuiji ZHOU0Tingzhao LI1Bo LI2Amway (Shanghai) Technology Development Co., Ltd., Shanghai 201203, ChinaAmway (Shanghai) Technology Development Co., Ltd., Shanghai 201203, ChinaAmway (Shanghai) Technology Development Co., Ltd., Shanghai 201203, ChinaObjective: To investigate and analyze antioxidant activities and tyrosinase inhibition of the aqueous extract of Osmanthus fragrans flower (O. fragrans), as well as its active ingredients. Methods: In this study, antioxidant activities of extract of O. fragrans were carried out by using DPPH·, ABTS+· radical scavenging assays and total antioxidant activity (FRAP). Qualitative analysis of major active components was performed by UPLC-PDA-QDa-ABTS+· online method and UF-LC-MS method were used to qualitatively identify the antioxidant components and tyrosinase inhibitors from O. fragrans extract respectively. Oxidative damage model of skin fibroblasts induced by H2O2 was used to evaluate the protective effect of oxidative stress of O. fragrans aqueous extract and its chemical components. Results: Aqueous extract of O. fragrans had good scavenging effect on DPPH· and ABTS+· free radical in vitro, and its EC50 values of free radical scavenging effect on DPPH· and ABTS+· were 37.66, 38.32 μg/mL, respectively. When the concentration of O. fragrans extract reached 2 mg/mL, FRAP value could be as high as 3.17. The EC50 of tyrosinase diphenoloxidase inhibition of O. fragrans extract was 595.9 μg/mL. Through analysis of UPLC-Triple-TOF/MS, a total of 28 chemical compositions were preliminarily identified. Five antioxidant active compounds which also greatly combined with tyrosinase were screened out from O. fragrans extract by simultaneously using UPLC-PDA-QDa-ABTS+· online method and UF-LC-MS method. Among them, the main active ingredient was verbascoside. Antioxidant and tyrosinase assay showed that verbascoside had significant antioxidant activity and tyrosinase inhibitory activity. EC50 values of verbascoside of scavenging effect on DPPH· and ABTS+· were 10.27, 14.96 μg/mL, respectively. When the concentration of verbascoside was 1 mg/mL, FRAP value could be up to 4.22. The EC50 of tyrosinase monophenoloxidase inhibition of verbascoside was 477.5 μg/mL, but with little diphenoloxidase inhibitiory effect. The oxidative damage model induced by H2O2 was used to further verify the significant protective effects of O. fragrans extract and verbascoside on oxidative damage of skin fibroblasts. Conclusion: The aqueous extract of O. fragrans and its main active component, verbascoside, had good antioxidant activity, tyrosinase inhibitory activity and oxidative stress protection ability.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070130osmanthus fragrans lour.verbascosideuplc-abts+·online antioxidationuf-lc-mstyrosinase inhibitors |
| spellingShingle | Huiji ZHOU Tingzhao LI Bo LI Identification of Antioxidant Components and Tyrosinase Specific Inhibitors from Osmanthus fragrans Flower by Using Online UPLC-ABTS+·-assay and UF- LC-MS Technology Shipin gongye ke-ji osmanthus fragrans lour. verbascoside uplc-abts+· online antioxidation uf-lc-ms tyrosinase inhibitors |
| title | Identification of Antioxidant Components and Tyrosinase Specific Inhibitors from Osmanthus fragrans Flower by Using Online UPLC-ABTS+·-assay and UF- LC-MS Technology |
| title_full | Identification of Antioxidant Components and Tyrosinase Specific Inhibitors from Osmanthus fragrans Flower by Using Online UPLC-ABTS+·-assay and UF- LC-MS Technology |
| title_fullStr | Identification of Antioxidant Components and Tyrosinase Specific Inhibitors from Osmanthus fragrans Flower by Using Online UPLC-ABTS+·-assay and UF- LC-MS Technology |
| title_full_unstemmed | Identification of Antioxidant Components and Tyrosinase Specific Inhibitors from Osmanthus fragrans Flower by Using Online UPLC-ABTS+·-assay and UF- LC-MS Technology |
| title_short | Identification of Antioxidant Components and Tyrosinase Specific Inhibitors from Osmanthus fragrans Flower by Using Online UPLC-ABTS+·-assay and UF- LC-MS Technology |
| title_sort | identification of antioxidant components and tyrosinase specific inhibitors from osmanthus fragrans flower by using online uplc abts · assay and uf lc ms technology |
| topic | osmanthus fragrans lour. verbascoside uplc-abts+· online antioxidation uf-lc-ms tyrosinase inhibitors |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070130 |
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