Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells
To study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vect...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100512&lng=en&tlng=en |
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| author | Dai Wei LI Shi Yun Xiao Dejun Liu Cong Jin-Hua He Lin Yan |
| author_facet | Dai Wei LI Shi Yun Xiao Dejun Liu Cong Jin-Hua He Lin Yan |
| author_sort | Dai Wei |
| collection | DOAJ |
| description | To study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The relative expression level of MALAT1 was detected by RT-qPCR. The protein expression was determined by Western blot analysis. The IC50 of curcumin alone was 77.69 mmol/L, which was 51.17 mol/L when combined with curcumin and random sequence. The IC50 of curcumin was 30.02 mmol/L when combined with si-MALAT1. The increased susceptibility multiples was 2.58. The wound healing rates were 30.9% and 67.5% after treatment with si-MALAT1 combined with curcumin for 24 hrs and 48 hrs, respectively. The numbers of invasion cells were 200±12, 162±13, 66±8, 53±4 and 16±3 after treatment with si-MALAT1 combined with curcumin for 48 hrs. The relative expression level of lncRNA-MALAT1 in the curcumin group was 68%, and the relative expression level of lncRNA-MALAT1 in si-MALAT1group was 56%, and that for the combination treatment group was about 21%. The protein expression levels of β- catenin, c-myc and cyclinD1 were significantly down-regulated upon treatment with certain concentration of si-MALAT1 alone or combined with curcumin.si-MALAT1 could significantly inhibit the invasion and migration of SW480 cells by enhancing the sensitivity of SW480 cells to curcumin. The mechanism involved mignt be related to the down-regulation of β-catenin, c-myc and cyclinD1 proteins. |
| first_indexed | 2024-12-18T10:09:30Z |
| format | Article |
| id | doaj.art-9241617fe2234d53bdfa9c07684da6c8 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-18T10:09:30Z |
| publisher | Universidade de São Paulo |
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| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-9241617fe2234d53bdfa9c07684da6c82022-12-21T21:11:28ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97905510.1590/s2175-97902019000118276S1984-82502019000100512Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cellsDai WeiLI Shi YunXiao DejunLiu CongJin-Hua HeLin YanTo study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The relative expression level of MALAT1 was detected by RT-qPCR. The protein expression was determined by Western blot analysis. The IC50 of curcumin alone was 77.69 mmol/L, which was 51.17 mol/L when combined with curcumin and random sequence. The IC50 of curcumin was 30.02 mmol/L when combined with si-MALAT1. The increased susceptibility multiples was 2.58. The wound healing rates were 30.9% and 67.5% after treatment with si-MALAT1 combined with curcumin for 24 hrs and 48 hrs, respectively. The numbers of invasion cells were 200±12, 162±13, 66±8, 53±4 and 16±3 after treatment with si-MALAT1 combined with curcumin for 48 hrs. The relative expression level of lncRNA-MALAT1 in the curcumin group was 68%, and the relative expression level of lncRNA-MALAT1 in si-MALAT1group was 56%, and that for the combination treatment group was about 21%. The protein expression levels of β- catenin, c-myc and cyclinD1 were significantly down-regulated upon treatment with certain concentration of si-MALAT1 alone or combined with curcumin.si-MALAT1 could significantly inhibit the invasion and migration of SW480 cells by enhancing the sensitivity of SW480 cells to curcumin. The mechanism involved mignt be related to the down-regulation of β-catenin, c-myc and cyclinD1 proteins.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100512&lng=en&tlng=enCurcumin/ pharmacologyLncRNA-MALAT1RNASmall Interfering/ drug effectsColon cancerInvasionMigrationColonic Neoplasms/ prevention & controlColonic Neoplasms/ drug therapyNeoplasm Invasiveness/ prevention & controlCell Migration Inhibition/ drug effects |
| spellingShingle | Dai Wei LI Shi Yun Xiao Dejun Liu Cong Jin-Hua He Lin Yan Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells Brazilian Journal of Pharmaceutical Sciences Curcumin/ pharmacology LncRNA-MALAT1 RNA Small Interfering/ drug effects Colon cancer Invasion Migration Colonic Neoplasms/ prevention & control Colonic Neoplasms/ drug therapy Neoplasm Invasiveness/ prevention & control Cell Migration Inhibition/ drug effects |
| title | Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
| title_full | Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
| title_fullStr | Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
| title_full_unstemmed | Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
| title_short | Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells |
| title_sort | curcumin combining with si malat1 inhibits the invasion and migration of colon cancer sw480 cells |
| topic | Curcumin/ pharmacology LncRNA-MALAT1 RNA Small Interfering/ drug effects Colon cancer Invasion Migration Colonic Neoplasms/ prevention & control Colonic Neoplasms/ drug therapy Neoplasm Invasiveness/ prevention & control Cell Migration Inhibition/ drug effects |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100512&lng=en&tlng=en |
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