Isolation and characterization of Dehydrin promoter region from sugarcane
The development of molecular biology techniques nowadays has enabled to engineer drought tolerant sugarcane by genetic engineering to accelerate the breeding program. Dehydrin (DHN) is known to have an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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indonesian research institute for biotechnology and bioindustry
2020-04-01
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| Series: | Menara Perkebunan |
| Subjects: | |
| Online Access: | http://mp.iribb.org/index.php/mpjurnal/article/view/363 |
| _version_ | 1818215332326670336 |
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| author | Hayati Minarsih Sonny Suhandono Anissa K Fuadi Tati Kristianti Riza A Putranto Deden Sukmadjaya Sustiprajitno |
| author_facet | Hayati Minarsih Sonny Suhandono Anissa K Fuadi Tati Kristianti Riza A Putranto Deden Sukmadjaya Sustiprajitno |
| author_sort | Hayati Minarsih |
| collection | DOAJ |
| description | The development of molecular biology techniques nowadays has enabled to engineer drought tolerant sugarcane by genetic engineering to accelerate the breeding program. Dehydrin (DHN) is known to have an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). While plant tissues are subjected to drought stress (dehydration), DHN protein is accumulated to high content throughout all vegetative or generative tissues. The research aimed to isolate and characterize the DHN promoter from sugarcane that can be used as transformation material in generating drought tolerant sugarcane. Specific primers for DHN promoter amplification were designed and DHN promoter region was successfully isolated by PCR cloning method. Two putative promoter sequences were identified namely Pr-1DHNSo and Pr-2DHNSo. In silicoanalyses were carried out and cis-regulatory elements motifs that play a role in adaptation on abiotic stress as well as biotic stress including ABRE, MBS, CGTCA-motif, TGACG-motif, GARE-motif, P-box TCA-element and Box-W1 were identified. The promoter Pr-1DHNSo was then cloned into pBI121 expression vector by Overlap Extention PCR (OE-PCR) for further characterization. Functional test of the promoter construct pBI- Pr-1DHNSo was conducted through Agrobacterium transformation into sugarcane calli. GUS assay and PCR analysis showed that the DHN promoter was transformed and expressed in the sugarcane calli. |
| first_indexed | 2024-12-12T06:34:24Z |
| format | Article |
| id | doaj.art-9241b65d32334dcb88fce685a425ace1 |
| institution | Directory Open Access Journal |
| issn | 0125-9318 0125-9318 |
| language | English |
| last_indexed | 2024-12-12T06:34:24Z |
| publishDate | 2020-04-01 |
| publisher | indonesian research institute for biotechnology and bioindustry |
| record_format | Article |
| series | Menara Perkebunan |
| spelling | doaj.art-9241b65d32334dcb88fce685a425ace12022-12-22T00:34:31Zengindonesian research institute for biotechnology and bioindustryMenara Perkebunan0125-93180125-93182020-04-01881162810.22302/iribb.jur.mp.v88i1.363Isolation and characterization of Dehydrin promoter region from sugarcaneHayati Minarsih0Sonny Suhandono1Anissa K Fuadi2Tati Kristianti3Riza A Putranto4Deden Sukmadjaya5Sustiprajitno6Indonesian Research Institute for Biotechnology and Bioindustry Institut Teknologi BandungInstitut Teknologi BandungIndonesian Institute of EducationIndonesian Research Institute for Biotechnology and Bioindustry Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and DevelopmentIndonesian Center for Agricultural Biotechnology and Genetic Resource Research and DevelopmentThe development of molecular biology techniques nowadays has enabled to engineer drought tolerant sugarcane by genetic engineering to accelerate the breeding program. Dehydrin (DHN) is known to have an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). While plant tissues are subjected to drought stress (dehydration), DHN protein is accumulated to high content throughout all vegetative or generative tissues. The research aimed to isolate and characterize the DHN promoter from sugarcane that can be used as transformation material in generating drought tolerant sugarcane. Specific primers for DHN promoter amplification were designed and DHN promoter region was successfully isolated by PCR cloning method. Two putative promoter sequences were identified namely Pr-1DHNSo and Pr-2DHNSo. In silicoanalyses were carried out and cis-regulatory elements motifs that play a role in adaptation on abiotic stress as well as biotic stress including ABRE, MBS, CGTCA-motif, TGACG-motif, GARE-motif, P-box TCA-element and Box-W1 were identified. The promoter Pr-1DHNSo was then cloned into pBI121 expression vector by Overlap Extention PCR (OE-PCR) for further characterization. Functional test of the promoter construct pBI- Pr-1DHNSo was conducted through Agrobacterium transformation into sugarcane calli. GUS assay and PCR analysis showed that the DHN promoter was transformed and expressed in the sugarcane calli.http://mp.iribb.org/index.php/mpjurnal/article/view/363drought stressdhnpromotersaccharum sp |
| spellingShingle | Hayati Minarsih Sonny Suhandono Anissa K Fuadi Tati Kristianti Riza A Putranto Deden Sukmadjaya Sustiprajitno Isolation and characterization of Dehydrin promoter region from sugarcane Menara Perkebunan drought stress dhn promoter saccharum sp |
| title | Isolation and characterization of Dehydrin promoter region from sugarcane |
| title_full | Isolation and characterization of Dehydrin promoter region from sugarcane |
| title_fullStr | Isolation and characterization of Dehydrin promoter region from sugarcane |
| title_full_unstemmed | Isolation and characterization of Dehydrin promoter region from sugarcane |
| title_short | Isolation and characterization of Dehydrin promoter region from sugarcane |
| title_sort | isolation and characterization of dehydrin promoter region from sugarcane |
| topic | drought stress dhn promoter saccharum sp |
| url | http://mp.iribb.org/index.php/mpjurnal/article/view/363 |
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