First report of Cytauxzoon sp. infection in domestic cats in Switzerland: natural and transfusion-transmitted infections
Abstract Background Cytauxzoonosis is an emerging tick-borne disease of domestic and wild felids. Cytauxzoon felis induces severe and often fatal disease in domestic cats. In Europe, clinical and subclinical infections caused by Cytauxzoon sp. are described. We report the first cases of Cytauxzoon s...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://link.springer.com/article/10.1186/s13071-018-2728-5 |
| _version_ | 1818436028299476992 |
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| author | Alice Nentwig Marina L. Meli Johanna Schrack Iris M. Reichler Barbara Riond Corinne Gloor Judith Howard Regina Hofmann-Lehmann Barbara Willi |
| author_facet | Alice Nentwig Marina L. Meli Johanna Schrack Iris M. Reichler Barbara Riond Corinne Gloor Judith Howard Regina Hofmann-Lehmann Barbara Willi |
| author_sort | Alice Nentwig |
| collection | DOAJ |
| description | Abstract Background Cytauxzoonosis is an emerging tick-borne disease of domestic and wild felids. Cytauxzoon felis induces severe and often fatal disease in domestic cats. In Europe, clinical and subclinical infections caused by Cytauxzoon sp. are described. We report the first cases of Cytauxzoon sp. infection in domestic cats in Switzerland. Methods Clinical and laboratory data and results of PCR analyses were collected from Cytauxzoon sp. PCR-positive cats and the cats followed for up to 851 days. Results The cases were three two-month old kittens from the same litter (Cases 1–3) and two adult domestic shorthair cats (Cases 4 and 5). The cats originated from the north-west and west of Switzerland. Cases 1–3 presented with moderate to severe regenerative anaemia and intraerythrocytic inclusions. Cytauxzoon sp. was confirmed by PCR and sequencing. The kittens made a clinical and haematological recovery after blood transfusion and/or treatment with azithromycin and atovaquone, but erythroparasitaemia persisted. Case 4 presented with severe non-regenerative anaemia. Case 5 was healthy and used as a blood donor for Case 4. Following blood transfusion, Case 4 showed intraerythrocytic inclusions, and Cytauxzoon sp. was confirmed in both Cases 4 and 5 using PCR and sequencing. Case 4 achieved clinical and haematological remission after treatment with azithromycin, atovaquone and immunosuppressive drugs. Eight months later, Case 4 was presented again with anaemia but tested Cytauxzoon sp. PCR-negative. Sequencing of 1637 bp of the 18S rRNA gene of Cytauxzoon sp. revealed 100% nucleotide sequence identity among isolates of Cases 1–3 and between isolates of Cases 4 and 5, and 99% sequence identity between isolates of all cases. Phylogenetic analysis revealed the closest relationship of the Swiss isolates to Cytauxzoon sp. isolates from domestic cats and wild felids from France, Spain and Romania and to Cytauxzoon manul from a Pallas’s cat. Conclusions This is the first report of Cytauxzoon sp. infection in domestic cats in Switzerland. It is also the first report of infection in very young kittens and transmission of Cytauxzoon sp. to an adult cat by transfusion of blood from an asymptomatic cat. The cats recovered but some developed chronic asymptomatic erythroparasitaemia for up to 28 months. Domestic cats may act as reservoirs for Cytauxzoon sp. in Europe and blood donor cats should be screened for this agent by PCR. |
| first_indexed | 2024-12-14T17:02:16Z |
| format | Article |
| id | doaj.art-9241e7d89dad409cb3bc11bec1f22e38 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-14T17:02:16Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
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| series | Parasites & Vectors |
| spelling | doaj.art-9241e7d89dad409cb3bc11bec1f22e382022-12-21T22:53:49ZengBMCParasites & Vectors1756-33052018-05-0111111310.1186/s13071-018-2728-5First report of Cytauxzoon sp. infection in domestic cats in Switzerland: natural and transfusion-transmitted infectionsAlice Nentwig0Marina L. Meli1Johanna Schrack2Iris M. Reichler3Barbara Riond4Corinne Gloor5Judith Howard6Regina Hofmann-Lehmann7Barbara Willi8Division of Small Animal Internal Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of BernClinical Laboratory, Vetsuisse Faculty, University of ZurichClinic of Reproductive Medicine, Vetsuisse Faculty, University of ZurichClinic of Reproductive Medicine, Vetsuisse Faculty, University of ZurichClinical Laboratory, Vetsuisse Faculty, University of ZurichDivision of Small Animal Internal Medicine, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of BernClinical Diagnostic Laboratory, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of BernClinical Laboratory, Vetsuisse Faculty, University of ZurichClinical Laboratory, Vetsuisse Faculty, University of ZurichAbstract Background Cytauxzoonosis is an emerging tick-borne disease of domestic and wild felids. Cytauxzoon felis induces severe and often fatal disease in domestic cats. In Europe, clinical and subclinical infections caused by Cytauxzoon sp. are described. We report the first cases of Cytauxzoon sp. infection in domestic cats in Switzerland. Methods Clinical and laboratory data and results of PCR analyses were collected from Cytauxzoon sp. PCR-positive cats and the cats followed for up to 851 days. Results The cases were three two-month old kittens from the same litter (Cases 1–3) and two adult domestic shorthair cats (Cases 4 and 5). The cats originated from the north-west and west of Switzerland. Cases 1–3 presented with moderate to severe regenerative anaemia and intraerythrocytic inclusions. Cytauxzoon sp. was confirmed by PCR and sequencing. The kittens made a clinical and haematological recovery after blood transfusion and/or treatment with azithromycin and atovaquone, but erythroparasitaemia persisted. Case 4 presented with severe non-regenerative anaemia. Case 5 was healthy and used as a blood donor for Case 4. Following blood transfusion, Case 4 showed intraerythrocytic inclusions, and Cytauxzoon sp. was confirmed in both Cases 4 and 5 using PCR and sequencing. Case 4 achieved clinical and haematological remission after treatment with azithromycin, atovaquone and immunosuppressive drugs. Eight months later, Case 4 was presented again with anaemia but tested Cytauxzoon sp. PCR-negative. Sequencing of 1637 bp of the 18S rRNA gene of Cytauxzoon sp. revealed 100% nucleotide sequence identity among isolates of Cases 1–3 and between isolates of Cases 4 and 5, and 99% sequence identity between isolates of all cases. Phylogenetic analysis revealed the closest relationship of the Swiss isolates to Cytauxzoon sp. isolates from domestic cats and wild felids from France, Spain and Romania and to Cytauxzoon manul from a Pallas’s cat. Conclusions This is the first report of Cytauxzoon sp. infection in domestic cats in Switzerland. It is also the first report of infection in very young kittens and transmission of Cytauxzoon sp. to an adult cat by transfusion of blood from an asymptomatic cat. The cats recovered but some developed chronic asymptomatic erythroparasitaemia for up to 28 months. Domestic cats may act as reservoirs for Cytauxzoon sp. in Europe and blood donor cats should be screened for this agent by PCR.http://link.springer.com/article/10.1186/s13071-018-2728-5CatCytauxzoonPiroplasmProtozoanTheileridaeTick-borne disease |
| spellingShingle | Alice Nentwig Marina L. Meli Johanna Schrack Iris M. Reichler Barbara Riond Corinne Gloor Judith Howard Regina Hofmann-Lehmann Barbara Willi First report of Cytauxzoon sp. infection in domestic cats in Switzerland: natural and transfusion-transmitted infections Parasites & Vectors Cat Cytauxzoon Piroplasm Protozoan Theileridae Tick-borne disease |
| title | First report of Cytauxzoon sp. infection in domestic cats in Switzerland: natural and transfusion-transmitted infections |
| title_full | First report of Cytauxzoon sp. infection in domestic cats in Switzerland: natural and transfusion-transmitted infections |
| title_fullStr | First report of Cytauxzoon sp. infection in domestic cats in Switzerland: natural and transfusion-transmitted infections |
| title_full_unstemmed | First report of Cytauxzoon sp. infection in domestic cats in Switzerland: natural and transfusion-transmitted infections |
| title_short | First report of Cytauxzoon sp. infection in domestic cats in Switzerland: natural and transfusion-transmitted infections |
| title_sort | first report of cytauxzoon sp infection in domestic cats in switzerland natural and transfusion transmitted infections |
| topic | Cat Cytauxzoon Piroplasm Protozoan Theileridae Tick-borne disease |
| url | http://link.springer.com/article/10.1186/s13071-018-2728-5 |
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