Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stages
Eyespot is one of the most important fungal diseases of the stem base of wheat (Triticum aestivum L.). The presented study clearly demonstrated that the Pch1 gene was the main effective source for reducing the eyespot disease score in the analyzed winter wheat lines. Nevertheless, Pch1 was presen...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2018-12-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/jppr.2018.124651 |
| _version_ | 1818085620159873024 |
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| author | Maciej Majka Michał Kwiatek Marek Korbas Jakub Danielewicz Magdalena Gawłowska Tomasz Góral Halina Wiśniewska |
| author_facet | Maciej Majka Michał Kwiatek Marek Korbas Jakub Danielewicz Magdalena Gawłowska Tomasz Góral Halina Wiśniewska |
| author_sort | Maciej Majka |
| collection | DOAJ |
| description | Eyespot is one of the most important fungal diseases of the stem base of wheat (Triticum
aestivum L.). The presented study clearly demonstrated that the Pch1 gene was the main
effective source for reducing the eyespot disease score in the analyzed winter wheat lines.
Nevertheless, Pch1 was present only in 8−9% of the investigated lines. Using an isoenzymatic
marker and molecular markers, the presence of the Pch1 gene and lack of the Pch2
gene was identified in six lines. Two lines, SMH 9409 and DL 358/13/4, were polymorphic
in an isoenzymatic marker study. In the remaining three lines, C 3373/11-1, KBH 15.15 and
KBP 1416, the Pch1 gene was identified only with the use of an isoenzymatic marker. Both
genes Pch1 and Pch2, as well as the resistant variety Rendezvous, were found in three lines:
DD 248/12, KBP 15.2 and STH 4431. In line DD 708/13, the presence of the Pch1 and Pch2
genes was identified, where the association between the Pch1 and the locus of the Xorw5
marker was broken. It was shown that the presence or absence of Pch1 and Pch2 genes did
not significantly affect the grain yield (from the plot), although the yield was highest in the
presence of both genes. A significant effect of the presence of the Pch1 gene on thousand
kernel weight (TKW) was observed. Lines with the Pch1 gene showed significantly higher
TKW values than lines without both genes or with the Pch2 gene only. |
| first_indexed | 2024-12-10T20:12:40Z |
| format | Article |
| id | doaj.art-9242bbc27aa942298947d643e07df15d |
| institution | Directory Open Access Journal |
| issn | 1899-007X 1899-007X |
| language | English |
| last_indexed | 2024-12-10T20:12:40Z |
| publishDate | 2018-12-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-9242bbc27aa942298947d643e07df15d2022-12-22T01:35:15ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2018-12-0158438739410.24425/jppr.2018.124651Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stagesMaciej Majka0Michał Kwiatek1Marek Korbas2Jakub Danielewicz3Magdalena Gawłowska4Tomasz Góral5Halina Wiśniewska6Department of Genomics, Institute of Plant Genetics Polish Academy of Sciences, Poznań, PolandDepartment of Genomics, Institute of Plant Genetics Polish Academy of Sciences, Poznań, PolandDepartment of Mycology, Institute of Plant Protection, National Research Institute, Poznań, PolandDepartment of Mycology, Institute of Plant Protection, National Research Institute, Poznań, PolandDepartment of Genomics, Institute of Plant Genetics Polish Academy of Sciences, Poznań, PolandDepartment of Phytopathology, Plant Breeding and Acclimatization Institute, National Research Institute, Poznań, PolandDepartment of Genomics, Institute of Plant Genetics Polish Academy of Sciences, Poznań, PolandEyespot is one of the most important fungal diseases of the stem base of wheat (Triticum aestivum L.). The presented study clearly demonstrated that the Pch1 gene was the main effective source for reducing the eyespot disease score in the analyzed winter wheat lines. Nevertheless, Pch1 was present only in 8−9% of the investigated lines. Using an isoenzymatic marker and molecular markers, the presence of the Pch1 gene and lack of the Pch2 gene was identified in six lines. Two lines, SMH 9409 and DL 358/13/4, were polymorphic in an isoenzymatic marker study. In the remaining three lines, C 3373/11-1, KBH 15.15 and KBP 1416, the Pch1 gene was identified only with the use of an isoenzymatic marker. Both genes Pch1 and Pch2, as well as the resistant variety Rendezvous, were found in three lines: DD 248/12, KBP 15.2 and STH 4431. In line DD 708/13, the presence of the Pch1 and Pch2 genes was identified, where the association between the Pch1 and the locus of the Xorw5 marker was broken. It was shown that the presence or absence of Pch1 and Pch2 genes did not significantly affect the grain yield (from the plot), although the yield was highest in the presence of both genes. A significant effect of the presence of the Pch1 gene on thousand kernel weight (TKW) was observed. Lines with the Pch1 gene showed significantly higher TKW values than lines without both genes or with the Pch2 gene only.https://doi.org/10.24425/jppr.2018.124651eyespotmarker-assisted selectionOculimacularesistancewheat |
| spellingShingle | Maciej Majka Michał Kwiatek Marek Korbas Jakub Danielewicz Magdalena Gawłowska Tomasz Góral Halina Wiśniewska Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stages Journal of Plant Protection Research eyespot marker-assisted selection Oculimacula resistance wheat |
| title | Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stages |
| title_full | Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stages |
| title_fullStr | Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stages |
| title_full_unstemmed | Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stages |
| title_short | Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stages |
| title_sort | eyespot resistance of winter wheat breeding lines evaluated with marker assisted selection and inoculation tests at the seedling and adult plant stages |
| topic | eyespot marker-assisted selection Oculimacula resistance wheat |
| url | https://doi.org/10.24425/jppr.2018.124651 |
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