Novel NARC-G1 garlic: comparative allicin quantification with morpho-biochemical & genetic profiling
Abstract Garlic (Allium sativum) is an important cash food crop, and the biotechnology industry has considerable interest in the plant because of its medicinal importance. These medicinal properties are attributed to organosulphur compounds as the accumulation of these compounds varies according to...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2022-08-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100373&tlng=en |
| _version_ | 1798039871759056896 |
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| author | S. Shamshad K. A. Rizvi H. Shehnaz S. A. Jatoi L. Shahnaz S. M. S. Naqvi M. Qasim T. Zainab A. Haider |
| author_facet | S. Shamshad K. A. Rizvi H. Shehnaz S. A. Jatoi L. Shahnaz S. M. S. Naqvi M. Qasim T. Zainab A. Haider |
| author_sort | S. Shamshad |
| collection | DOAJ |
| description | Abstract Garlic (Allium sativum) is an important cash food crop, and the biotechnology industry has considerable interest in the plant because of its medicinal importance. These medicinal properties are attributed to organosulphur compounds as the accumulation of these compounds varies according to genotype, locality, light quality, and cultivation practices. In this study, we compared a newly developed garlic variety NARC-G1 by National Agricultural Research Centre (NARC), Islamabad, Pakistan with three different garlic cultivars and highlighted the distinctive attributes like phenotypic characteristics, the content of allicin, elemental profile, and gene polymorphism. Phenotypic analysis showed NARC-G1 has significantly higher bulb weight (66.36g ± 18.58), single clove weight (5.87g ± 1.041), and clove width (17.41mm ± 0.95) which directly correlates to the size of the garlic. The analytical analysis showed the highest allicin content (4.82 ± 0.001) in NARC-G1. Genotyping of the alliinase in all four cultivars showed indels in the gene resulting in distinguishable changes in organosulphur compounds’ profile. NARC-G1 is unique from other garlic cultivars and could be the best choice for mass production with proper cultivation and irrigation management. Moreover, for Pakistan NARC-G1 could be a potential contender to earn the industrial benefits with inland cultivation instead of importing garlic alleviating the economic burden. |
| first_indexed | 2024-04-11T21:59:39Z |
| format | Article |
| id | doaj.art-9245632858844381857c7bef23d001bd |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-11T21:59:39Z |
| publishDate | 2022-08-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-9245632858844381857c7bef23d001bd2022-12-22T04:00:59ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752022-08-018410.1590/1519-6984.262697Novel NARC-G1 garlic: comparative allicin quantification with morpho-biochemical & genetic profilingS. Shamshadhttps://orcid.org/0000-0001-7890-7081K. A. Rizvihttps://orcid.org/0000-0002-3782-2716H. Shehnazhttps://orcid.org/0000-0003-3003-6453S. A. Jatoihttps://orcid.org/0000-0002-5715-1016L. Shahnazhttps://orcid.org/0000-0002-1635-2030S. M. S. Naqvihttps://orcid.org/0000-0002-5059-7461M. Qasimhttps://orcid.org/0000-0003-1717-1917T. Zainabhttps://orcid.org/0000-0003-0902-4909A. Haiderhttps://orcid.org/0000-0002-8813-3291Abstract Garlic (Allium sativum) is an important cash food crop, and the biotechnology industry has considerable interest in the plant because of its medicinal importance. These medicinal properties are attributed to organosulphur compounds as the accumulation of these compounds varies according to genotype, locality, light quality, and cultivation practices. In this study, we compared a newly developed garlic variety NARC-G1 by National Agricultural Research Centre (NARC), Islamabad, Pakistan with three different garlic cultivars and highlighted the distinctive attributes like phenotypic characteristics, the content of allicin, elemental profile, and gene polymorphism. Phenotypic analysis showed NARC-G1 has significantly higher bulb weight (66.36g ± 18.58), single clove weight (5.87g ± 1.041), and clove width (17.41mm ± 0.95) which directly correlates to the size of the garlic. The analytical analysis showed the highest allicin content (4.82 ± 0.001) in NARC-G1. Genotyping of the alliinase in all four cultivars showed indels in the gene resulting in distinguishable changes in organosulphur compounds’ profile. NARC-G1 is unique from other garlic cultivars and could be the best choice for mass production with proper cultivation and irrigation management. Moreover, for Pakistan NARC-G1 could be a potential contender to earn the industrial benefits with inland cultivation instead of importing garlic alleviating the economic burden.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100373&tlng=engarlic (Allium sativum)alliinase geneNARC-G1 garlicICP-OESHPLC-based allicin quantification |
| spellingShingle | S. Shamshad K. A. Rizvi H. Shehnaz S. A. Jatoi L. Shahnaz S. M. S. Naqvi M. Qasim T. Zainab A. Haider Novel NARC-G1 garlic: comparative allicin quantification with morpho-biochemical & genetic profiling Brazilian Journal of Biology garlic (Allium sativum) alliinase gene NARC-G1 garlic ICP-OES HPLC-based allicin quantification |
| title | Novel NARC-G1 garlic: comparative allicin quantification with morpho-biochemical & genetic profiling |
| title_full | Novel NARC-G1 garlic: comparative allicin quantification with morpho-biochemical & genetic profiling |
| title_fullStr | Novel NARC-G1 garlic: comparative allicin quantification with morpho-biochemical & genetic profiling |
| title_full_unstemmed | Novel NARC-G1 garlic: comparative allicin quantification with morpho-biochemical & genetic profiling |
| title_short | Novel NARC-G1 garlic: comparative allicin quantification with morpho-biochemical & genetic profiling |
| title_sort | novel narc g1 garlic comparative allicin quantification with morpho biochemical genetic profiling |
| topic | garlic (Allium sativum) alliinase gene NARC-G1 garlic ICP-OES HPLC-based allicin quantification |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100373&tlng=en |
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