Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes
The postpartum (PP) period is a crucial stage for the resumption of reproductive performance and ovarian cyclicity in dairy buffaloes. The present study aimed, for the first time, to assess the effect of the administration of estradiol benzoate (EB) on ovarian and uterine hemodynamics in PP dairy bu...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/14/2340 |
| _version_ | 1797590588622635008 |
|---|---|
| author | Haney Samir Hossam R. El-Sherbiny Ahmed Ezzat Ahmed Ramya Ahmad Sindi Khalid M. Al Syaad Elshymaa A. Abdelnaby |
| author_facet | Haney Samir Hossam R. El-Sherbiny Ahmed Ezzat Ahmed Ramya Ahmad Sindi Khalid M. Al Syaad Elshymaa A. Abdelnaby |
| author_sort | Haney Samir |
| collection | DOAJ |
| description | The postpartum (PP) period is a crucial stage for the resumption of reproductive performance and ovarian cyclicity in dairy buffaloes. The present study aimed, for the first time, to assess the effect of the administration of estradiol benzoate (EB) on ovarian and uterine hemodynamics in PP dairy buffaloes. Eight pluriparous acyclic domestic buffaloes were enrolled in the present experiment and received a dose of 10 mg of estradiol benzoate (EB) intramuscularly 4 weeks after parturition. All animals were examined two times before EB administration (days −3, and −1) and on the day of EB administration (day 0), followed by examinations on days 1, 3, 5, 7, and 9 post-EB administration. The middle uterine artery (MUA) and ovarian artery (OA) blood flow patterns were assessed using a color Doppler ultrasound device. The reproductive parameters were (1) the cross-sectional diameters (cm) of the OA and MUA, (2) cranial uterine horn thickness (UHT; cm), and (3) hemodynamic changes within the MUA on both the ipsi- and contra-lateral sides of the previous pregnant horn and within the OA corresponding to the ovarian tissues. The examined blood flow parameters were the pulsatility index (PI), resistance index (RI), peak systolic/end-diastolic ratio (S/D), time-averaged maximum velocity (TAV; cm/s), uterine blood flow rate (BFR; bpm), and uterine blood flow volume (BFV; mL/min). Concomitantly, blood samples were collected from the coccygeal vein, and the sera were stored at −18 °C for use in estradiol (E2-17β) and nitric oxide (NO) assays. The results revealed increases in both OA and MUA cross-sectional diameter (cm) on the ipsi-lateral and contra-lateral (<i>p</i> < 0.05) sides within 24 h until day 9 post-treatment. The values of the RI and PI of blood flow within the OA and MUA on the ipsi-lateral and contra-lateral sides of the previous pregnancy were obviously lower (<i>p</i> < 0.05) at 24 h after the administration of EB, and then, started to gradually elevate, reaching the pre-treatment values on day 9 after EB administration. Both the BFR and BFV in the OA and MUA significantly increased from 24 h to 72 h after EB administration on both the ipsi-lateral and contra-lateral sides (<i>p</i> < 0.05); then, their values started to decrease to reach the pretreatment value on day 9 after EB administration. Both E2 and NO concentrations significantly increased (<i>p</i> < 0.05) from 24 h until day 3 after EB injection and then started to decline after that, reaching the pre-treatment value on day 9. In conclusion, the administration of EB enhances the ovarian and uterine blood flow concomitantly with increased levels of NO in PP dairy buffaloes. |
| first_indexed | 2024-03-11T01:22:41Z |
| format | Article |
| id | doaj.art-9248511bf93244158fc5833f25c3d842 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:22:41Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-9248511bf93244158fc5833f25c3d8422023-11-18T18:01:02ZengMDPI AGAnimals2076-26152023-07-011314234010.3390/ani13142340Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy BuffaloesHaney Samir0Hossam R. El-Sherbiny1Ahmed Ezzat Ahmed2Ramya Ahmad Sindi3Khalid M. Al Syaad4Elshymaa A. Abdelnaby5Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptTheriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptBiology Department, College of Science, King Khalid University, Abha 61413, Saudi ArabiaDepartment of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah Al-Mukarramah 24382, Saudi ArabiaBiology Department, College of Science, King Khalid University, Abha 61413, Saudi ArabiaTheriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptThe postpartum (PP) period is a crucial stage for the resumption of reproductive performance and ovarian cyclicity in dairy buffaloes. The present study aimed, for the first time, to assess the effect of the administration of estradiol benzoate (EB) on ovarian and uterine hemodynamics in PP dairy buffaloes. Eight pluriparous acyclic domestic buffaloes were enrolled in the present experiment and received a dose of 10 mg of estradiol benzoate (EB) intramuscularly 4 weeks after parturition. All animals were examined two times before EB administration (days −3, and −1) and on the day of EB administration (day 0), followed by examinations on days 1, 3, 5, 7, and 9 post-EB administration. The middle uterine artery (MUA) and ovarian artery (OA) blood flow patterns were assessed using a color Doppler ultrasound device. The reproductive parameters were (1) the cross-sectional diameters (cm) of the OA and MUA, (2) cranial uterine horn thickness (UHT; cm), and (3) hemodynamic changes within the MUA on both the ipsi- and contra-lateral sides of the previous pregnant horn and within the OA corresponding to the ovarian tissues. The examined blood flow parameters were the pulsatility index (PI), resistance index (RI), peak systolic/end-diastolic ratio (S/D), time-averaged maximum velocity (TAV; cm/s), uterine blood flow rate (BFR; bpm), and uterine blood flow volume (BFV; mL/min). Concomitantly, blood samples were collected from the coccygeal vein, and the sera were stored at −18 °C for use in estradiol (E2-17β) and nitric oxide (NO) assays. The results revealed increases in both OA and MUA cross-sectional diameter (cm) on the ipsi-lateral and contra-lateral (<i>p</i> < 0.05) sides within 24 h until day 9 post-treatment. The values of the RI and PI of blood flow within the OA and MUA on the ipsi-lateral and contra-lateral sides of the previous pregnancy were obviously lower (<i>p</i> < 0.05) at 24 h after the administration of EB, and then, started to gradually elevate, reaching the pre-treatment values on day 9 after EB administration. Both the BFR and BFV in the OA and MUA significantly increased from 24 h to 72 h after EB administration on both the ipsi-lateral and contra-lateral sides (<i>p</i> < 0.05); then, their values started to decrease to reach the pretreatment value on day 9 after EB administration. Both E2 and NO concentrations significantly increased (<i>p</i> < 0.05) from 24 h until day 3 after EB injection and then started to decline after that, reaching the pre-treatment value on day 9. In conclusion, the administration of EB enhances the ovarian and uterine blood flow concomitantly with increased levels of NO in PP dairy buffaloes.https://www.mdpi.com/2076-2615/13/14/2340buffaloesestradiol benzoatecolor Doppler ultrasonographynitric oxideovarian and uterine hemodynamicspostpartum |
| spellingShingle | Haney Samir Hossam R. El-Sherbiny Ahmed Ezzat Ahmed Ramya Ahmad Sindi Khalid M. Al Syaad Elshymaa A. Abdelnaby Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes Animals buffaloes estradiol benzoate color Doppler ultrasonography nitric oxide ovarian and uterine hemodynamics postpartum |
| title | Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes |
| title_full | Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes |
| title_fullStr | Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes |
| title_full_unstemmed | Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes |
| title_short | Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes |
| title_sort | administration of estradiol benzoate enhances ovarian and uterine hemodynamics in postpartum dairy buffaloes |
| topic | buffaloes estradiol benzoate color Doppler ultrasonography nitric oxide ovarian and uterine hemodynamics postpartum |
| url | https://www.mdpi.com/2076-2615/13/14/2340 |
| work_keys_str_mv | AT haneysamir administrationofestradiolbenzoateenhancesovariananduterinehemodynamicsinpostpartumdairybuffaloes AT hossamrelsherbiny administrationofestradiolbenzoateenhancesovariananduterinehemodynamicsinpostpartumdairybuffaloes AT ahmedezzatahmed administrationofestradiolbenzoateenhancesovariananduterinehemodynamicsinpostpartumdairybuffaloes AT ramyaahmadsindi administrationofestradiolbenzoateenhancesovariananduterinehemodynamicsinpostpartumdairybuffaloes AT khalidmalsyaad administrationofestradiolbenzoateenhancesovariananduterinehemodynamicsinpostpartumdairybuffaloes AT elshymaaaabdelnaby administrationofestradiolbenzoateenhancesovariananduterinehemodynamicsinpostpartumdairybuffaloes |