The first study of genetic diversity and population structure of Indo- Pacific bottlenose dolphin (Tursiops aduncus) and pantropical spotted dolphin (Stenella attenuata) in the Thai Andaman Sea based on ISSR
Background and Aim: The Indo-Pacific bottlenose dolphin, Tursiops aduncus, and the pantropical spotted dolphin, Stenella attenuata, are protected marine mammals in Thailand; however, knowledge regarding the populations of both species in Thai seas is minimal. We aimed to reveal the genetic diversity...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2022-08-01
|
| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.15/August-2022/13.pdf |
| _version_ | 1828759431117012992 |
|---|---|
| author | Promporn Piboon Anocha Poommouang Kittisak Buddhachat Patcharaporn Kaewmong Kongkiat Kittiwattanawong Korakot Nganvongpanit |
| author_facet | Promporn Piboon Anocha Poommouang Kittisak Buddhachat Patcharaporn Kaewmong Kongkiat Kittiwattanawong Korakot Nganvongpanit |
| author_sort | Promporn Piboon |
| collection | DOAJ |
| description | Background and Aim: The Indo-Pacific bottlenose dolphin, Tursiops aduncus, and the pantropical spotted dolphin, Stenella attenuata, are protected marine mammals in Thailand; however, knowledge regarding the populations of both species in Thai seas is minimal. We aimed to reveal the genetic diversity and population structure of two species, T. aduncus, and S. attenuata, based on inter-simple sequence repeats (ISSRs).
Materials and Methods: Samples of stranded T. aduncus (n = 30) and S. attenuata (n = 23) found along Thai Andaman Sea coasts from 1998 to 2018 were used in this study. A total of 17 and 16 ISSR primers that produced clear and polymorphic bands were selected for T. aduncus and S. attenuata, respectively.
Results: The highest percentages of polymorphic bands for T. aduncus and S. attenuata were 93.750% and 92.857%, respectively. Phylogenetic dendrograms indicated that the population of each species was clustered into three groups. This outcome was consistent with the genetic population structure, as both suggested three genetic clusters (ΔK = 3). Genetic diversity analysis revealed that the average Shannon's information index (I) was 1.926 ± 0.066 for T. aduncus and 1.714 ± 0.090 for S. attenuata, which indicate a high level of genetic variation. Further, low fixation index (F) values were observed for T. aduncus and S. attenuata at –0.231 ± 0.024 and –0.312 ± 0.042, respectively, suggesting that inbreeding is unlikely to have occurred for both species over the past decades.
Conclusion: At least three genetic clusters of both species were found in the Thai Andaman Sea, and the diversity indices of each species indicated that these species are not at a critical level for extinction. However, monitoring their population status should be prioritized to observe any future changes in the level of diversity. |
| first_indexed | 2024-12-11T00:57:13Z |
| format | Article |
| id | doaj.art-92489c6dd84545e0a4c537b409dd9702 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-11T00:57:13Z |
| publishDate | 2022-08-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-92489c6dd84545e0a4c537b409dd97022022-12-22T01:26:26ZengVeterinary WorldVeterinary World0972-89882231-09162022-08-011582004201110.14202/vetworld.2022.2004-2011The first study of genetic diversity and population structure of Indo- Pacific bottlenose dolphin (Tursiops aduncus) and pantropical spotted dolphin (Stenella attenuata) in the Thai Andaman Sea based on ISSRPromporn Piboon0https://orcid.org/0000-0002-2879-8611Anocha Poommouang1https://orcid.org/0000-0003-2260-0042Kittisak Buddhachat2https://orcid.org/0000-0003-4746-9861Patcharaporn Kaewmong3https://orcid.org/0000-0003-1826-2554Kongkiat Kittiwattanawong4https://orcid.org/0000-0003-2899-1910Korakot Nganvongpanit5https://orcid.org/0000-0002-7617-5507Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.Excellence Center in Veterinary Bioscience, Chiang Mai 50100, Thailand; Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.Phuket Marine Biological Center, Phuket 83000, Thailand.Phuket Marine Biological Center, Phuket 83000, Thailand.Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand; Excellence Center in Veterinary Bioscience, Chiang Mai 50100, Thailand.Background and Aim: The Indo-Pacific bottlenose dolphin, Tursiops aduncus, and the pantropical spotted dolphin, Stenella attenuata, are protected marine mammals in Thailand; however, knowledge regarding the populations of both species in Thai seas is minimal. We aimed to reveal the genetic diversity and population structure of two species, T. aduncus, and S. attenuata, based on inter-simple sequence repeats (ISSRs). Materials and Methods: Samples of stranded T. aduncus (n = 30) and S. attenuata (n = 23) found along Thai Andaman Sea coasts from 1998 to 2018 were used in this study. A total of 17 and 16 ISSR primers that produced clear and polymorphic bands were selected for T. aduncus and S. attenuata, respectively. Results: The highest percentages of polymorphic bands for T. aduncus and S. attenuata were 93.750% and 92.857%, respectively. Phylogenetic dendrograms indicated that the population of each species was clustered into three groups. This outcome was consistent with the genetic population structure, as both suggested three genetic clusters (ΔK = 3). Genetic diversity analysis revealed that the average Shannon's information index (I) was 1.926 ± 0.066 for T. aduncus and 1.714 ± 0.090 for S. attenuata, which indicate a high level of genetic variation. Further, low fixation index (F) values were observed for T. aduncus and S. attenuata at –0.231 ± 0.024 and –0.312 ± 0.042, respectively, suggesting that inbreeding is unlikely to have occurred for both species over the past decades. Conclusion: At least three genetic clusters of both species were found in the Thai Andaman Sea, and the diversity indices of each species indicated that these species are not at a critical level for extinction. However, monitoring their population status should be prioritized to observe any future changes in the level of diversity.http://www.veterinaryworld.org/Vol.15/August-2022/13.pdfcetaceansdiversityindo-pacific bottlenose dolphinpantropical spotted dolphinpopulation structure |
| spellingShingle | Promporn Piboon Anocha Poommouang Kittisak Buddhachat Patcharaporn Kaewmong Kongkiat Kittiwattanawong Korakot Nganvongpanit The first study of genetic diversity and population structure of Indo- Pacific bottlenose dolphin (Tursiops aduncus) and pantropical spotted dolphin (Stenella attenuata) in the Thai Andaman Sea based on ISSR Veterinary World cetaceans diversity indo-pacific bottlenose dolphin pantropical spotted dolphin population structure |
| title | The first study of genetic diversity and population structure of Indo- Pacific bottlenose dolphin (Tursiops aduncus) and pantropical spotted dolphin (Stenella attenuata) in the Thai Andaman Sea based on ISSR |
| title_full | The first study of genetic diversity and population structure of Indo- Pacific bottlenose dolphin (Tursiops aduncus) and pantropical spotted dolphin (Stenella attenuata) in the Thai Andaman Sea based on ISSR |
| title_fullStr | The first study of genetic diversity and population structure of Indo- Pacific bottlenose dolphin (Tursiops aduncus) and pantropical spotted dolphin (Stenella attenuata) in the Thai Andaman Sea based on ISSR |
| title_full_unstemmed | The first study of genetic diversity and population structure of Indo- Pacific bottlenose dolphin (Tursiops aduncus) and pantropical spotted dolphin (Stenella attenuata) in the Thai Andaman Sea based on ISSR |
| title_short | The first study of genetic diversity and population structure of Indo- Pacific bottlenose dolphin (Tursiops aduncus) and pantropical spotted dolphin (Stenella attenuata) in the Thai Andaman Sea based on ISSR |
| title_sort | first study of genetic diversity and population structure of indo pacific bottlenose dolphin tursiops aduncus and pantropical spotted dolphin stenella attenuata in the thai andaman sea based on issr |
| topic | cetaceans diversity indo-pacific bottlenose dolphin pantropical spotted dolphin population structure |
| url | http://www.veterinaryworld.org/Vol.15/August-2022/13.pdf |
| work_keys_str_mv | AT prompornpiboon thefirststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT anochapoommouang thefirststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT kittisakbuddhachat thefirststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT patcharapornkaewmong thefirststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT kongkiatkittiwattanawong thefirststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT korakotnganvongpanit thefirststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT prompornpiboon firststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT anochapoommouang firststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT kittisakbuddhachat firststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT patcharapornkaewmong firststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT kongkiatkittiwattanawong firststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr AT korakotnganvongpanit firststudyofgeneticdiversityandpopulationstructureofindopacificbottlenosedolphintursiopsaduncusandpantropicalspotteddolphinstenellaattenuatainthethaiandamanseabasedonissr |