Bacterial Dimethylsulfoniopropionate Biosynthesis in the East China Sea
Dimethylsulfoniopropionate (DMSP) is one of Earth’s most abundant organosulfur molecules. Recently, many marine heterotrophic bacteria were shown to produce DMSP, but few studies have combined culture-dependent and independent techniques to study their abundance, distribution, diversity and activity...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/3/657 |
| _version_ | 1797540370041536512 |
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| author | Ji Liu Yunhui Zhang Jingli Liu Haohui Zhong Beth T. Williams Yanfen Zheng Andrew R. J. Curson Chuang Sun Hao Sun Delei Song Brett Wagner Mackenzie Ana Bermejo Martínez Jonathan D. Todd Xiao-Hua Zhang |
| author_facet | Ji Liu Yunhui Zhang Jingli Liu Haohui Zhong Beth T. Williams Yanfen Zheng Andrew R. J. Curson Chuang Sun Hao Sun Delei Song Brett Wagner Mackenzie Ana Bermejo Martínez Jonathan D. Todd Xiao-Hua Zhang |
| author_sort | Ji Liu |
| collection | DOAJ |
| description | Dimethylsulfoniopropionate (DMSP) is one of Earth’s most abundant organosulfur molecules. Recently, many marine heterotrophic bacteria were shown to produce DMSP, but few studies have combined culture-dependent and independent techniques to study their abundance, distribution, diversity and activity in seawater or sediment environments. Here we investigate bacterial DMSP production potential in East China Sea (ECS) samples. Total DMSP (DMSPt) concentration in ECS seawater was highest in surface waters (SW) where phytoplankton were most abundant, and it decreased with depth to near bottom waters. However, the percentage of DMSPt mainly apportioned to bacteria increased from the surface to the near bottom water. The highest DMSP concentration was detected in ECS oxic surface sediment (OSS) where phytoplankton were not abundant. Bacteria with the genetic potential to produce DMSP and relevant biosynthesis gene transcripts were prominent in all ECS seawater and sediment samples. Their abundance also increased with depth and was highest in the OSS samples. Microbial enrichments for DMSP-producing bacteria from sediment and seawater identified many novel taxonomic groups of DMSP-producing bacteria. Different profiles of DMSP-producing bacteria existed between seawater and sediment samples and there are still novel DMSP-producing bacterial groups to be discovered in these environments. This study shows that heterotrophic bacteria significantly contribute to the marine DMSP pool and that their contribution increases with water depth and is highest in seabed surface sediment where DMSP catabolic potential is lowest. Furthermore, distinct bacterial groups likely produce DMSP in seawater and sediment samples, and many novel producing taxa exist, especially in the sediment. |
| first_indexed | 2024-03-10T13:00:08Z |
| format | Article |
| id | doaj.art-924a1001a5a7403099d96bb59bb53612 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:00:08Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-924a1001a5a7403099d96bb59bb536122023-11-21T11:34:57ZengMDPI AGMicroorganisms2076-26072021-03-019365710.3390/microorganisms9030657Bacterial Dimethylsulfoniopropionate Biosynthesis in the East China SeaJi Liu0Yunhui Zhang1Jingli Liu2Haohui Zhong3Beth T. Williams4Yanfen Zheng5Andrew R. J. Curson6Chuang Sun7Hao Sun8Delei Song9Brett Wagner Mackenzie10Ana Bermejo Martínez11Jonathan D. Todd12Xiao-Hua Zhang13College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaSchool of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UKCollege of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaSchool of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UKCollege of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaDepartment of Surgery, School of Medicine, The University of Auckland, Auckland 1142, New ZealandSchool of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UKCollege of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, ChinaDimethylsulfoniopropionate (DMSP) is one of Earth’s most abundant organosulfur molecules. Recently, many marine heterotrophic bacteria were shown to produce DMSP, but few studies have combined culture-dependent and independent techniques to study their abundance, distribution, diversity and activity in seawater or sediment environments. Here we investigate bacterial DMSP production potential in East China Sea (ECS) samples. Total DMSP (DMSPt) concentration in ECS seawater was highest in surface waters (SW) where phytoplankton were most abundant, and it decreased with depth to near bottom waters. However, the percentage of DMSPt mainly apportioned to bacteria increased from the surface to the near bottom water. The highest DMSP concentration was detected in ECS oxic surface sediment (OSS) where phytoplankton were not abundant. Bacteria with the genetic potential to produce DMSP and relevant biosynthesis gene transcripts were prominent in all ECS seawater and sediment samples. Their abundance also increased with depth and was highest in the OSS samples. Microbial enrichments for DMSP-producing bacteria from sediment and seawater identified many novel taxonomic groups of DMSP-producing bacteria. Different profiles of DMSP-producing bacteria existed between seawater and sediment samples and there are still novel DMSP-producing bacterial groups to be discovered in these environments. This study shows that heterotrophic bacteria significantly contribute to the marine DMSP pool and that their contribution increases with water depth and is highest in seabed surface sediment where DMSP catabolic potential is lowest. Furthermore, distinct bacterial groups likely produce DMSP in seawater and sediment samples, and many novel producing taxa exist, especially in the sediment.https://www.mdpi.com/2076-2607/9/3/657DMSP biosynthesissedimentbacteria |
| spellingShingle | Ji Liu Yunhui Zhang Jingli Liu Haohui Zhong Beth T. Williams Yanfen Zheng Andrew R. J. Curson Chuang Sun Hao Sun Delei Song Brett Wagner Mackenzie Ana Bermejo Martínez Jonathan D. Todd Xiao-Hua Zhang Bacterial Dimethylsulfoniopropionate Biosynthesis in the East China Sea Microorganisms DMSP biosynthesis sediment bacteria |
| title | Bacterial Dimethylsulfoniopropionate Biosynthesis in the East China Sea |
| title_full | Bacterial Dimethylsulfoniopropionate Biosynthesis in the East China Sea |
| title_fullStr | Bacterial Dimethylsulfoniopropionate Biosynthesis in the East China Sea |
| title_full_unstemmed | Bacterial Dimethylsulfoniopropionate Biosynthesis in the East China Sea |
| title_short | Bacterial Dimethylsulfoniopropionate Biosynthesis in the East China Sea |
| title_sort | bacterial dimethylsulfoniopropionate biosynthesis in the east china sea |
| topic | DMSP biosynthesis sediment bacteria |
| url | https://www.mdpi.com/2076-2607/9/3/657 |
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