Induced Expression of <i>CYP51a</i> and <i>HK1</i> Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen <i>Fusarium oxysporum</i>
Preventing antifungal resistance development and identifying pathogens with high, medium, and low risk of resistance development to a particular fungicide or fungicide class is crucial in the fight against phytopathogens. We characterized the sensitivity of potato wilt-associated <i>Fusarium o...
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/5/1257 |
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| author | Yaw A. Akosah Zarina S. Kostennikova Marat T. Lutfullin Guzel F. Lutfullina Daniel M. Afordoanyi Semyon G. Vologin Ayslu M. Mardanova |
| author_facet | Yaw A. Akosah Zarina S. Kostennikova Marat T. Lutfullin Guzel F. Lutfullina Daniel M. Afordoanyi Semyon G. Vologin Ayslu M. Mardanova |
| author_sort | Yaw A. Akosah |
| collection | DOAJ |
| description | Preventing antifungal resistance development and identifying pathogens with high, medium, and low risk of resistance development to a particular fungicide or fungicide class is crucial in the fight against phytopathogens. We characterized the sensitivity of potato wilt-associated <i>Fusarium oxysporum</i> isolates to fludioxonil and penconazole and assessed the effect of these fungicides on the expression of fungal sterol-14-α-demethylase (<i>CYP51a)</i> and histidine kinase (<i>HK1</i>) genes. Penconazole stunted the growth of <i>F. oxysporum</i> strains at all concentrations used. While all isolates were susceptible to this fungicide, concentrations of up to 1.0 μg/mL were insufficient to cause a 50% inhibition. At low concentrations (0.63 and 1.25 μg/mL), fludioxonil stimulated growth in <i>F. oxysporum</i>. With an increase in the concentration of fludioxonil, only one strain (<i>F. oxysporum</i> S95) exhibited moderate sensitivity to the fungicide. Interaction of <i>F. oxysporum</i> with penconazole and fludioxonil leads to respective elevated expressions of the <i>CYP51a</i> and <i>HK1</i> genes, which upsurge with increasing concentration of the fungicides. The data obtained indicate that fludioxonil may no longer be suitable for potato protection and its continuous use could only lead to an increased resistance with time. |
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| language | English |
| last_indexed | 2024-03-11T03:28:34Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-924ac0860c824f73a348116b2702f1f22023-11-18T02:34:07ZengMDPI AGMicroorganisms2076-26072023-05-01115125710.3390/microorganisms11051257Induced Expression of <i>CYP51a</i> and <i>HK1</i> Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen <i>Fusarium oxysporum</i>Yaw A. Akosah0Zarina S. Kostennikova1Marat T. Lutfullin2Guzel F. Lutfullina3Daniel M. Afordoanyi4Semyon G. Vologin5Ayslu M. Mardanova6Department of Molecular Pathology, New York University College of Dentistry, New York, NY 10010, USALaboratory of Microbial Biotechnology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, RussiaLaboratory of Microbial Biotechnology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, RussiaLaboratory of Microbial Biotechnology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, RussiaDepartment of Agrobiological Research, Tatar Scientific Research Institute of Agricultural Chemistry and Soil Science, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan 420059, RussiaDepartment of Agrochemical and Biochemical Analysis, Tatar Research Institute of Agriculture, Kazan Scientific Center of Russian Academy of Sciences, Kazan 420059, RussiaLaboratory of Microbial Biotechnology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, RussiaPreventing antifungal resistance development and identifying pathogens with high, medium, and low risk of resistance development to a particular fungicide or fungicide class is crucial in the fight against phytopathogens. We characterized the sensitivity of potato wilt-associated <i>Fusarium oxysporum</i> isolates to fludioxonil and penconazole and assessed the effect of these fungicides on the expression of fungal sterol-14-α-demethylase (<i>CYP51a)</i> and histidine kinase (<i>HK1</i>) genes. Penconazole stunted the growth of <i>F. oxysporum</i> strains at all concentrations used. While all isolates were susceptible to this fungicide, concentrations of up to 1.0 μg/mL were insufficient to cause a 50% inhibition. At low concentrations (0.63 and 1.25 μg/mL), fludioxonil stimulated growth in <i>F. oxysporum</i>. With an increase in the concentration of fludioxonil, only one strain (<i>F. oxysporum</i> S95) exhibited moderate sensitivity to the fungicide. Interaction of <i>F. oxysporum</i> with penconazole and fludioxonil leads to respective elevated expressions of the <i>CYP51a</i> and <i>HK1</i> genes, which upsurge with increasing concentration of the fungicides. The data obtained indicate that fludioxonil may no longer be suitable for potato protection and its continuous use could only lead to an increased resistance with time.https://www.mdpi.com/2076-2607/11/5/1257fludioxonilpenconazole<i>Fusarium</i><i>Solanum tuberosum</i> L.wiltdry rot |
| spellingShingle | Yaw A. Akosah Zarina S. Kostennikova Marat T. Lutfullin Guzel F. Lutfullina Daniel M. Afordoanyi Semyon G. Vologin Ayslu M. Mardanova Induced Expression of <i>CYP51a</i> and <i>HK1</i> Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen <i>Fusarium oxysporum</i> Microorganisms fludioxonil penconazole <i>Fusarium</i> <i>Solanum tuberosum</i> L. wilt dry rot |
| title | Induced Expression of <i>CYP51a</i> and <i>HK1</i> Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen <i>Fusarium oxysporum</i> |
| title_full | Induced Expression of <i>CYP51a</i> and <i>HK1</i> Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen <i>Fusarium oxysporum</i> |
| title_fullStr | Induced Expression of <i>CYP51a</i> and <i>HK1</i> Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen <i>Fusarium oxysporum</i> |
| title_full_unstemmed | Induced Expression of <i>CYP51a</i> and <i>HK1</i> Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen <i>Fusarium oxysporum</i> |
| title_short | Induced Expression of <i>CYP51a</i> and <i>HK1</i> Genes Associated with Penconazole and Fludioxonil Resistance in the Potato Pathogen <i>Fusarium oxysporum</i> |
| title_sort | induced expression of i cyp51a i and i hk1 i genes associated with penconazole and fludioxonil resistance in the potato pathogen i fusarium oxysporum i |
| topic | fludioxonil penconazole <i>Fusarium</i> <i>Solanum tuberosum</i> L. wilt dry rot |
| url | https://www.mdpi.com/2076-2607/11/5/1257 |
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