Cumulus Cell Transcriptome after Cumulus-Oocyte Complex Exposure to Nanomolar Cadmium in an In Vitro Animal Model of Prepubertal and Adult Age
Cadmium (Cd), a highly toxic pollutant, impairs oocyte fertilization, through oxidative damage on cumulus cells (CCs). This study analysed the transcriptomic profile of CCs of cumulus-oocyte complexes (COCs) from adult and prepubertal sheep, exposed to Cd nanomolar concentration during in vitro matu...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2079-7737/12/2/249 |
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| author | Nicola Antonio Martino Ernesto Picardi Elena Ciani Anna Maria D’Erchia Luisa Bogliolo Federica Ariu Antonella Mastrorocco Letizia Temerario Luigi Mansi Valeria Palumbo Graziano Pesole Maria Elena Dell’Aquila |
| author_facet | Nicola Antonio Martino Ernesto Picardi Elena Ciani Anna Maria D’Erchia Luisa Bogliolo Federica Ariu Antonella Mastrorocco Letizia Temerario Luigi Mansi Valeria Palumbo Graziano Pesole Maria Elena Dell’Aquila |
| author_sort | Nicola Antonio Martino |
| collection | DOAJ |
| description | Cadmium (Cd), a highly toxic pollutant, impairs oocyte fertilization, through oxidative damage on cumulus cells (CCs). This study analysed the transcriptomic profile of CCs of cumulus-oocyte complexes (COCs) from adult and prepubertal sheep, exposed to Cd nanomolar concentration during in vitro maturation. In both age-groups, CCs of matured oocytes underwent RNA-seq, data analysis and validation. Differentially expressed genes (DEGs) were identified in adult (<i>n</i> = 99 DEGs) and prepubertal (<i>n</i> = 18 DEGs) CCs upon Cd exposure. Transcriptomes of adult CCs clustered separately between Cd-exposed and control samples, whereas prepubertal ones did not as observed by Principal Component Analysis. The transcriptomic signature of Cd-induced CC toxicity was identified by gene annotation and literature search. Genes associated with previous studies on ovarian functions and/or Cd effects were confirmed and new genes were identified, thus implementing the knowledge on their involvement in such processes. Enrichment and validation analysis showed that, in adult CCs, Cd acted as endocrine disruptor on DEGs involved in hormone biosynthesis, cumulus expansion, regulation of cell signalling, growth and differentiation and oocyte maturation, whereas in prepubertal CCs, Cd affected DEGs involved in CC development and viability and CC-oocyte communications. In conclusion, these DEGs could be used as valuable non-invasive biomarkers for oocyte competence. |
| first_indexed | 2024-03-11T09:08:08Z |
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| id | doaj.art-924c1a4d974045ffb9835dbaa97af931 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T09:08:08Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-924c1a4d974045ffb9835dbaa97af9312023-11-16T19:13:44ZengMDPI AGBiology2079-77372023-02-0112224910.3390/biology12020249Cumulus Cell Transcriptome after Cumulus-Oocyte Complex Exposure to Nanomolar Cadmium in an In Vitro Animal Model of Prepubertal and Adult AgeNicola Antonio Martino0Ernesto Picardi1Elena Ciani2Anna Maria D’Erchia3Luisa Bogliolo4Federica Ariu5Antonella Mastrorocco6Letizia Temerario7Luigi Mansi8Valeria Palumbo9Graziano Pesole10Maria Elena Dell’Aquila11Department of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Veterinary Medicine, University of Sassari, Via Vienna n. 2, 07100 Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, Via Vienna n. 2, 07100 Sassari, ItalyDepartment of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies & Environment, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyCadmium (Cd), a highly toxic pollutant, impairs oocyte fertilization, through oxidative damage on cumulus cells (CCs). This study analysed the transcriptomic profile of CCs of cumulus-oocyte complexes (COCs) from adult and prepubertal sheep, exposed to Cd nanomolar concentration during in vitro maturation. In both age-groups, CCs of matured oocytes underwent RNA-seq, data analysis and validation. Differentially expressed genes (DEGs) were identified in adult (<i>n</i> = 99 DEGs) and prepubertal (<i>n</i> = 18 DEGs) CCs upon Cd exposure. Transcriptomes of adult CCs clustered separately between Cd-exposed and control samples, whereas prepubertal ones did not as observed by Principal Component Analysis. The transcriptomic signature of Cd-induced CC toxicity was identified by gene annotation and literature search. Genes associated with previous studies on ovarian functions and/or Cd effects were confirmed and new genes were identified, thus implementing the knowledge on their involvement in such processes. Enrichment and validation analysis showed that, in adult CCs, Cd acted as endocrine disruptor on DEGs involved in hormone biosynthesis, cumulus expansion, regulation of cell signalling, growth and differentiation and oocyte maturation, whereas in prepubertal CCs, Cd affected DEGs involved in CC development and viability and CC-oocyte communications. In conclusion, these DEGs could be used as valuable non-invasive biomarkers for oocyte competence.https://www.mdpi.com/2079-7737/12/2/249adult sheepprepubertal lambcumulus celltranscriptomeRNA-sequencingcadmium |
| spellingShingle | Nicola Antonio Martino Ernesto Picardi Elena Ciani Anna Maria D’Erchia Luisa Bogliolo Federica Ariu Antonella Mastrorocco Letizia Temerario Luigi Mansi Valeria Palumbo Graziano Pesole Maria Elena Dell’Aquila Cumulus Cell Transcriptome after Cumulus-Oocyte Complex Exposure to Nanomolar Cadmium in an In Vitro Animal Model of Prepubertal and Adult Age Biology adult sheep prepubertal lamb cumulus cell transcriptome RNA-sequencing cadmium |
| title | Cumulus Cell Transcriptome after Cumulus-Oocyte Complex Exposure to Nanomolar Cadmium in an In Vitro Animal Model of Prepubertal and Adult Age |
| title_full | Cumulus Cell Transcriptome after Cumulus-Oocyte Complex Exposure to Nanomolar Cadmium in an In Vitro Animal Model of Prepubertal and Adult Age |
| title_fullStr | Cumulus Cell Transcriptome after Cumulus-Oocyte Complex Exposure to Nanomolar Cadmium in an In Vitro Animal Model of Prepubertal and Adult Age |
| title_full_unstemmed | Cumulus Cell Transcriptome after Cumulus-Oocyte Complex Exposure to Nanomolar Cadmium in an In Vitro Animal Model of Prepubertal and Adult Age |
| title_short | Cumulus Cell Transcriptome after Cumulus-Oocyte Complex Exposure to Nanomolar Cadmium in an In Vitro Animal Model of Prepubertal and Adult Age |
| title_sort | cumulus cell transcriptome after cumulus oocyte complex exposure to nanomolar cadmium in an in vitro animal model of prepubertal and adult age |
| topic | adult sheep prepubertal lamb cumulus cell transcriptome RNA-sequencing cadmium |
| url | https://www.mdpi.com/2079-7737/12/2/249 |
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