Change of Serum Metabolome and Cecal Microflora in Broiler Chickens Supplemented With Grape Seed Extracts
Grape seed is rich in vitamin E, flavonoids, and proanthocyanidins and has the potential to be used as an antibiotic substitute in broilers. We investigated the effects of grape seed proanthocyanidin extract (GSPE) on growth performance, immune responses, cecal microflora, and serum metabolism in ea...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.610934/full |
| _version_ | 1818728621633699840 |
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| author | Guangtian Cao Xinfu Zeng Jinsong Liu Feifei Yan Zhentian Xiang Yongxia Wang Fei Tao Caimei Yang |
| author_facet | Guangtian Cao Xinfu Zeng Jinsong Liu Feifei Yan Zhentian Xiang Yongxia Wang Fei Tao Caimei Yang |
| author_sort | Guangtian Cao |
| collection | DOAJ |
| description | Grape seed is rich in vitamin E, flavonoids, and proanthocyanidins and has the potential to be used as an antibiotic substitute in broilers. We investigated the effects of grape seed proanthocyanidin extract (GSPE) on growth performance, immune responses, cecal microflora, and serum metabolism in early stage broilers. Data indicated that GSPE improved broiler growth performance by strengthening antioxidant capacity, enhancing immune responses, and increasing cecal short chain fatty acids. 16S rRNA sequencing indicated that GSPE changed the predominant cecal microflora and induced the metabolism of amino acids, lipids, and carbohydrates. An UPLC-Q-TOF/MS-based metabolomics analysis identified 23 serum metabolites (mainly related to lipid, amino acid, and alkaloid) were extremely changed by GSPE treatment. The correlations between the changes of cecal microflora and serum metabolites in birds fed with GSPE were analyzed. Hence, GSPE potentially provides active ingredients that may be used as antibiotic substitute and reduces environmental pollution by grape by-products. |
| first_indexed | 2024-12-17T22:32:54Z |
| format | Article |
| id | doaj.art-924c1fd284ef44cf8f4c5dfce3666580 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-17T22:32:54Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-924c1fd284ef44cf8f4c5dfce36665802022-12-21T21:30:09ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-12-011110.3389/fimmu.2020.610934610934Change of Serum Metabolome and Cecal Microflora in Broiler Chickens Supplemented With Grape Seed ExtractsGuangtian Cao0Xinfu Zeng1Jinsong Liu2Feifei Yan3Zhentian Xiang4Yongxia Wang5Fei Tao6Caimei Yang7College of Standardization, China Jiliang University, Hangzhou, ChinaZhejiang Vegamax Biotechnology Co., Ltd., Anji, ChinaZhejiang Vegamax Biotechnology Co., Ltd., Anji, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, The Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, Zhejiang A & F University, Hangzhou, ChinaZhejiang Vegamax Biotechnology Co., Ltd., Anji, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, The Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, Zhejiang A & F University, Hangzhou, ChinaCollege of Standardization, China Jiliang University, Hangzhou, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, The Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, Zhejiang A & F University, Hangzhou, ChinaGrape seed is rich in vitamin E, flavonoids, and proanthocyanidins and has the potential to be used as an antibiotic substitute in broilers. We investigated the effects of grape seed proanthocyanidin extract (GSPE) on growth performance, immune responses, cecal microflora, and serum metabolism in early stage broilers. Data indicated that GSPE improved broiler growth performance by strengthening antioxidant capacity, enhancing immune responses, and increasing cecal short chain fatty acids. 16S rRNA sequencing indicated that GSPE changed the predominant cecal microflora and induced the metabolism of amino acids, lipids, and carbohydrates. An UPLC-Q-TOF/MS-based metabolomics analysis identified 23 serum metabolites (mainly related to lipid, amino acid, and alkaloid) were extremely changed by GSPE treatment. The correlations between the changes of cecal microflora and serum metabolites in birds fed with GSPE were analyzed. Hence, GSPE potentially provides active ingredients that may be used as antibiotic substitute and reduces environmental pollution by grape by-products.https://www.frontiersin.org/articles/10.3389/fimmu.2020.610934/fullanti-inflammatory effectproanthocyanidinsgrape seed extractcecal microfloraserum metabolomics |
| spellingShingle | Guangtian Cao Xinfu Zeng Jinsong Liu Feifei Yan Zhentian Xiang Yongxia Wang Fei Tao Caimei Yang Change of Serum Metabolome and Cecal Microflora in Broiler Chickens Supplemented With Grape Seed Extracts Frontiers in Immunology anti-inflammatory effect proanthocyanidins grape seed extract cecal microflora serum metabolomics |
| title | Change of Serum Metabolome and Cecal Microflora in Broiler Chickens Supplemented With Grape Seed Extracts |
| title_full | Change of Serum Metabolome and Cecal Microflora in Broiler Chickens Supplemented With Grape Seed Extracts |
| title_fullStr | Change of Serum Metabolome and Cecal Microflora in Broiler Chickens Supplemented With Grape Seed Extracts |
| title_full_unstemmed | Change of Serum Metabolome and Cecal Microflora in Broiler Chickens Supplemented With Grape Seed Extracts |
| title_short | Change of Serum Metabolome and Cecal Microflora in Broiler Chickens Supplemented With Grape Seed Extracts |
| title_sort | change of serum metabolome and cecal microflora in broiler chickens supplemented with grape seed extracts |
| topic | anti-inflammatory effect proanthocyanidins grape seed extract cecal microflora serum metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2020.610934/full |
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