The isolation and characterization of lipase from Carica papaya latex using zwitterion sodium lauroyl sarcosinate as agent
Most of industrial lipases are derived from microbial sources, following by a wide variety of plants. Among plant lipases, lipase from Carica papaya latex has been the focus of intense and growing research due to low cost, easy acceptance by consumers and its unique characteristics. This enzyme has...
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| Format: | Article |
| Language: | English |
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HACCP Consulting
2019-10-01
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| Series: | Potravinarstvo |
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| Online Access: | https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1164 |
| _version_ | 1818029496314363904 |
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| author | Dang Minh Nhat Phan Thi Viet Ha |
| author_facet | Dang Minh Nhat Phan Thi Viet Ha |
| author_sort | Dang Minh Nhat |
| collection | DOAJ |
| description | Most of industrial lipases are derived from microbial sources, following by a wide variety of plants. Among plant lipases, lipase from Carica papaya latex has been the focus of intense and growing research due to low cost, easy acceptance by consumers and its unique characteristics. This enzyme has been successfully applied for lipid modification and synthesis of some organic compounds. However, research for its molecular structure has been limited due to the difficulty to isolate the enzyme from the latex matrix. In this study, we suggested a modified approach using sodium lauroyl sarcosinate to solubilize the latex, then the protein was precipitated by ammonium sulphate. We also carried out the characterization of the lipase obtained from Carica papaya latex. The results showed that freeze-drying the fresh latex could improve significantly lipase activity of latex powder in comparison with sun-drying or oven-drying. The zwitterion sodium lauroyl sarcosinate could solubilize nearly 50% of the latex and the achieved supernatant exhibited great lipase activity. There was no need to use an organic solvent to delipidate the latex prior to solubilization with sodium lauroyl sarcosinate due to possible denaturation of enzymes. The proteins which were fractionally precipitated with 50 - 60%, 60 - 70% and 70 - 80% ammonium sulphate saturation showed lipolytic activity. The fraction from 50 - 60% saturation with the greatest mass was subjected to ion exchange chromatography, SDS electrophoresis and kinetic parameter determination. The results showed the presence of two proteins with molecular mass ranging from 35 kDa to 55 kDa and both presented lipase activity. The Km and Vmax of the lipase fraction from 50 - 60% saturation was 1.12 mM and 1.2 x 10-6 mM.min-1.mL-1 respectively. So, the freeze-drying of papaya latex could help to preserve its lipase activity and the usage of sodium lauroyl sarcosinate could improve the isolation of the lipase from the papaya latex and pave the way for research on the molecular structure of Carica papaya latex lipases. |
| first_indexed | 2024-12-10T05:20:37Z |
| format | Article |
| id | doaj.art-924e6fd05c774b13a440b36344ca6281 |
| institution | Directory Open Access Journal |
| issn | 1337-0960 |
| language | English |
| last_indexed | 2024-12-10T05:20:37Z |
| publishDate | 2019-10-01 |
| publisher | HACCP Consulting |
| record_format | Article |
| series | Potravinarstvo |
| spelling | doaj.art-924e6fd05c774b13a440b36344ca62812022-12-22T02:00:50ZengHACCP ConsultingPotravinarstvo1337-09602019-10-0113177377810.5219/1164855The isolation and characterization of lipase from Carica papaya latex using zwitterion sodium lauroyl sarcosinate as agentDang Minh Nhat0https://orcid.org/0000-0001-6515-1879Phan Thi Viet Ha1https://orcid.org/0000-0001-7686-6343The University of Da Nang ”“ University of Science and Technology, Faculty of Chemical Engineering, Department of Food Technology, 54 Nguyen Luong Bang, Da Nang City, Viet Nam, Tel.: +84 913 486 813Duy Tan University, Faculty of Natural Sciences, 03 Quang Trung, Da Nang City, Viet Nam, Tel.: +84 935 133 255Most of industrial lipases are derived from microbial sources, following by a wide variety of plants. Among plant lipases, lipase from Carica papaya latex has been the focus of intense and growing research due to low cost, easy acceptance by consumers and its unique characteristics. This enzyme has been successfully applied for lipid modification and synthesis of some organic compounds. However, research for its molecular structure has been limited due to the difficulty to isolate the enzyme from the latex matrix. In this study, we suggested a modified approach using sodium lauroyl sarcosinate to solubilize the latex, then the protein was precipitated by ammonium sulphate. We also carried out the characterization of the lipase obtained from Carica papaya latex. The results showed that freeze-drying the fresh latex could improve significantly lipase activity of latex powder in comparison with sun-drying or oven-drying. The zwitterion sodium lauroyl sarcosinate could solubilize nearly 50% of the latex and the achieved supernatant exhibited great lipase activity. There was no need to use an organic solvent to delipidate the latex prior to solubilization with sodium lauroyl sarcosinate due to possible denaturation of enzymes. The proteins which were fractionally precipitated with 50 - 60%, 60 - 70% and 70 - 80% ammonium sulphate saturation showed lipolytic activity. The fraction from 50 - 60% saturation with the greatest mass was subjected to ion exchange chromatography, SDS electrophoresis and kinetic parameter determination. The results showed the presence of two proteins with molecular mass ranging from 35 kDa to 55 kDa and both presented lipase activity. The Km and Vmax of the lipase fraction from 50 - 60% saturation was 1.12 mM and 1.2 x 10-6 mM.min-1.mL-1 respectively. So, the freeze-drying of papaya latex could help to preserve its lipase activity and the usage of sodium lauroyl sarcosinate could improve the isolation of the lipase from the papaya latex and pave the way for research on the molecular structure of Carica papaya latex lipases.https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1164lipasepapayaenzyme isolationenzyme purification |
| spellingShingle | Dang Minh Nhat Phan Thi Viet Ha The isolation and characterization of lipase from Carica papaya latex using zwitterion sodium lauroyl sarcosinate as agent Potravinarstvo lipase papaya enzyme isolation enzyme purification |
| title | The isolation and characterization of lipase from Carica papaya latex using zwitterion sodium lauroyl sarcosinate as agent |
| title_full | The isolation and characterization of lipase from Carica papaya latex using zwitterion sodium lauroyl sarcosinate as agent |
| title_fullStr | The isolation and characterization of lipase from Carica papaya latex using zwitterion sodium lauroyl sarcosinate as agent |
| title_full_unstemmed | The isolation and characterization of lipase from Carica papaya latex using zwitterion sodium lauroyl sarcosinate as agent |
| title_short | The isolation and characterization of lipase from Carica papaya latex using zwitterion sodium lauroyl sarcosinate as agent |
| title_sort | isolation and characterization of lipase from carica papaya latex using zwitterion sodium lauroyl sarcosinate as agent |
| topic | lipase papaya enzyme isolation enzyme purification |
| url | https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1164 |
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