Interaction between <i>Dickeya dianthicola</i> and <i>Pectobacterium parmentieri</i> in Potato Infection under Field Conditions
<i>Dickeya</i> and <i>Pectobacterium</i> spp. both cause blackleg and soft rot of potato, which can be a yield-reducing factor to potato production. The purpose of this study was to examine the interaction between these two bacterial genera causing potato infection, and subse...
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MDPI AG
2021-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/316 |
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| author | Tongling Ge Fatemeh Ekbataniamiri Steven B. Johnson Robert P. Larkin Jianjun Hao |
| author_facet | Tongling Ge Fatemeh Ekbataniamiri Steven B. Johnson Robert P. Larkin Jianjun Hao |
| author_sort | Tongling Ge |
| collection | DOAJ |
| description | <i>Dickeya</i> and <i>Pectobacterium</i> spp. both cause blackleg and soft rot of potato, which can be a yield-reducing factor to potato production. The purpose of this study was to examine the interaction between these two bacterial genera causing potato infection, and subsequent disease development and yield responses under field conditions. Analysis of 883 potato samples collected in Northeastern USA using polymerase chain reaction determined that <i>Dickeya dianthicola</i> and <i>P. parmentieri</i> were found in 38.1% and 53.3% of all samples, respectively, and that 20.6% of samples contained both <i>D. dianthicola</i> and <i>P. parmentieri</i>. To further investigate the relationship between the two bacterial species and their interaction, field trials were established. Potato seed pieces of “Russet Burbank”, “Lamoka”, and “Atlantic” were inoculated with bacterial suspension of <i>D. dianthicola</i> at 10<sup>7</sup> colony-forming unite (CFU)/mL using a vacuum infiltration method, air dried, and then planted in the field. Two-year results showed that there was a high correlation (<i>p</i> < 0.01) between yield loss and percent of inoculated seed pieces. In a secondary field trial conducted in 2018 and 2019, seed pieces of potato “Shepody”, “Lamoka” and “Atlantic” were inoculated with <i>D. dianthicola</i>, <i>P. parmentieri</i>, or mixture of both species, and then planted. In 2019, disease severity index, as measured by the most sensitive variety “Lamoka”, was 16.2 with <i>D. dianthicola</i> inoculation, 10.4 with <i>P. parmentieri</i>, 25.4 with inoculation with both bacteria. Two-year data had a similar trend. Thus, <i>D. dianthicola</i> was more virulent than <i>P</i>. <i>parmentieri</i>, but the co-inoculation of the two species resulted in increased disease severity compared to single-species inoculation with either pathogen. |
| first_indexed | 2024-03-09T05:48:17Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T05:48:17Z |
| publishDate | 2021-02-01 |
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| spelling | doaj.art-924f4fc0f79b45f2bcce8d400b59ea7d2023-12-03T12:19:08ZengMDPI AGMicroorganisms2076-26072021-02-019231610.3390/microorganisms9020316Interaction between <i>Dickeya dianthicola</i> and <i>Pectobacterium parmentieri</i> in Potato Infection under Field ConditionsTongling Ge0Fatemeh Ekbataniamiri1Steven B. Johnson2Robert P. Larkin3Jianjun Hao4School of Food and Agriculture, University of Maine, Orono, ME 04469, USASchool of Food and Agriculture, University of Maine, Orono, ME 04469, USACooperative Extension, University of Maine, Orono, ME 04469, USANew England Plant, Soil, and Water Lab, USDA-ARS, University of Maine, Orono, ME 04469, USASchool of Food and Agriculture, University of Maine, Orono, ME 04469, USA<i>Dickeya</i> and <i>Pectobacterium</i> spp. both cause blackleg and soft rot of potato, which can be a yield-reducing factor to potato production. The purpose of this study was to examine the interaction between these two bacterial genera causing potato infection, and subsequent disease development and yield responses under field conditions. Analysis of 883 potato samples collected in Northeastern USA using polymerase chain reaction determined that <i>Dickeya dianthicola</i> and <i>P. parmentieri</i> were found in 38.1% and 53.3% of all samples, respectively, and that 20.6% of samples contained both <i>D. dianthicola</i> and <i>P. parmentieri</i>. To further investigate the relationship between the two bacterial species and their interaction, field trials were established. Potato seed pieces of “Russet Burbank”, “Lamoka”, and “Atlantic” were inoculated with bacterial suspension of <i>D. dianthicola</i> at 10<sup>7</sup> colony-forming unite (CFU)/mL using a vacuum infiltration method, air dried, and then planted in the field. Two-year results showed that there was a high correlation (<i>p</i> < 0.01) between yield loss and percent of inoculated seed pieces. In a secondary field trial conducted in 2018 and 2019, seed pieces of potato “Shepody”, “Lamoka” and “Atlantic” were inoculated with <i>D. dianthicola</i>, <i>P. parmentieri</i>, or mixture of both species, and then planted. In 2019, disease severity index, as measured by the most sensitive variety “Lamoka”, was 16.2 with <i>D. dianthicola</i> inoculation, 10.4 with <i>P. parmentieri</i>, 25.4 with inoculation with both bacteria. Two-year data had a similar trend. Thus, <i>D. dianthicola</i> was more virulent than <i>P</i>. <i>parmentieri</i>, but the co-inoculation of the two species resulted in increased disease severity compared to single-species inoculation with either pathogen.https://www.mdpi.com/2076-2607/9/2/316vacuum infiltrationblackleg and soft rotsynergy |
| spellingShingle | Tongling Ge Fatemeh Ekbataniamiri Steven B. Johnson Robert P. Larkin Jianjun Hao Interaction between <i>Dickeya dianthicola</i> and <i>Pectobacterium parmentieri</i> in Potato Infection under Field Conditions Microorganisms vacuum infiltration blackleg and soft rot synergy |
| title | Interaction between <i>Dickeya dianthicola</i> and <i>Pectobacterium parmentieri</i> in Potato Infection under Field Conditions |
| title_full | Interaction between <i>Dickeya dianthicola</i> and <i>Pectobacterium parmentieri</i> in Potato Infection under Field Conditions |
| title_fullStr | Interaction between <i>Dickeya dianthicola</i> and <i>Pectobacterium parmentieri</i> in Potato Infection under Field Conditions |
| title_full_unstemmed | Interaction between <i>Dickeya dianthicola</i> and <i>Pectobacterium parmentieri</i> in Potato Infection under Field Conditions |
| title_short | Interaction between <i>Dickeya dianthicola</i> and <i>Pectobacterium parmentieri</i> in Potato Infection under Field Conditions |
| title_sort | interaction between i dickeya dianthicola i and i pectobacterium parmentieri i in potato infection under field conditions |
| topic | vacuum infiltration blackleg and soft rot synergy |
| url | https://www.mdpi.com/2076-2607/9/2/316 |
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