The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm
Unlike the classical nuclear envelope with two membranes found in other eukaryotic cells, most nematode sperm nuclei are not encapsulated by membranes. Instead, they are surrounded by a nuclear halo of unknown composition. How the halo is formed and regulated is unknown. We used forward genetics to...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2018-07-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.118.200426 |
| _version_ | 1819031993436864512 |
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| author | Leslie A. Herrera Daniel A. Starr |
| author_facet | Leslie A. Herrera Daniel A. Starr |
| author_sort | Leslie A. Herrera |
| collection | DOAJ |
| description | Unlike the classical nuclear envelope with two membranes found in other eukaryotic cells, most nematode sperm nuclei are not encapsulated by membranes. Instead, they are surrounded by a nuclear halo of unknown composition. How the halo is formed and regulated is unknown. We used forward genetics to identify molecular lesions behind three classical fer (fertilization defective) mutations that disrupt the ultrastructure of the Caenorhabditis elegans sperm nuclear halo. We found fer-2 and fer-4 alleles to be nonsense mutations in mib-1. fer-3 was caused by a nonsense mutation in eri-3. GFP::MIB-1 was expressed in the germline during early spermatogenesis, but not in mature sperm. mib-1 encodes a conserved E3 ubiquitin ligase homologous to vertebrate Mib1 and Mib2, which function in Notch signaling. Here, we show that mib-1 is important for male sterility and is involved in the regulation or formation of the nuclear halo during nematode spermatogenesis. |
| first_indexed | 2024-12-21T06:54:52Z |
| format | Article |
| id | doaj.art-924fd83bf4344723bf2a19cf351da143 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-21T06:54:52Z |
| publishDate | 2018-07-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-924fd83bf4344723bf2a19cf351da1432022-12-21T19:12:21ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362018-07-01872465247010.1534/g3.118.20042631The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans SpermLeslie A. HerreraDaniel A. StarrUnlike the classical nuclear envelope with two membranes found in other eukaryotic cells, most nematode sperm nuclei are not encapsulated by membranes. Instead, they are surrounded by a nuclear halo of unknown composition. How the halo is formed and regulated is unknown. We used forward genetics to identify molecular lesions behind three classical fer (fertilization defective) mutations that disrupt the ultrastructure of the Caenorhabditis elegans sperm nuclear halo. We found fer-2 and fer-4 alleles to be nonsense mutations in mib-1. fer-3 was caused by a nonsense mutation in eri-3. GFP::MIB-1 was expressed in the germline during early spermatogenesis, but not in mature sperm. mib-1 encodes a conserved E3 ubiquitin ligase homologous to vertebrate Mib1 and Mib2, which function in Notch signaling. Here, we show that mib-1 is important for male sterility and is involved in the regulation or formation of the nuclear halo during nematode spermatogenesis.http://g3journal.org/lookup/doi/10.1534/g3.118.200426spermatogenesisE3 ubiquitin ligasenuclear envelopeC. elegans |
| spellingShingle | Leslie A. Herrera Daniel A. Starr The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm G3: Genes, Genomes, Genetics spermatogenesis E3 ubiquitin ligase nuclear envelope C. elegans |
| title | The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm |
| title_full | The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm |
| title_fullStr | The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm |
| title_full_unstemmed | The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm |
| title_short | The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm |
| title_sort | e3 ubiquitin ligase mib 1 is necessary to form the nuclear halo in caenorhabditis elegans sperm |
| topic | spermatogenesis E3 ubiquitin ligase nuclear envelope C. elegans |
| url | http://g3journal.org/lookup/doi/10.1534/g3.118.200426 |
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