Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)
ABSTRACT The aim of this study was to evaluate the effect of ethanolic "aroeira" (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x103 cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium m...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2016-01-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100301&lng=en&tlng=en |
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| author | Alessandra Cury Machado Patrícia Fernandes Sartori Carla Andreotti Damante Anne Lígia Dokkedal Rodrigo Cardoso de Oliveira |
| author_facet | Alessandra Cury Machado Patrícia Fernandes Sartori Carla Andreotti Damante Anne Lígia Dokkedal Rodrigo Cardoso de Oliveira |
| author_sort | Alessandra Cury Machado |
| collection | DOAJ |
| description | ABSTRACT The aim of this study was to evaluate the effect of ethanolic "aroeira" (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x103 cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium modified by Dulbecco - DMEM) supplemented with 10% fetal bovine serum was replaced by DMEM with different ethanolic extract concentration (0, 0.1, 1, 10, 100, and 1000μg / mL). The fibroblast viability was analyzed after 48,72, and 96 h by the neutral red capture test and violet crystal. The "aroeira" extract, at high concentrations (100 and 1000 µg/mL) caused decrease in both cellular viability tests (p<0.05). However, dilutions between 0.1 and 10 µg/mL did not affect the viability of the cells. It was concluded that "aroeira" extract was able to change the gingival fibroblast viability, and this effect was concentration dependent. |
| first_indexed | 2024-12-21T22:19:36Z |
| format | Article |
| id | doaj.art-92500f974cc743ada6881cd5ed07cf1f |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-21T22:19:36Z |
| publishDate | 2016-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-92500f974cc743ada6881cd5ed07cf1f2022-12-21T18:48:22ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242016-01-0159010.1590/1678-4324-2016150335S1516-89132016000100301Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)Alessandra Cury MachadoPatrícia Fernandes SartoriCarla Andreotti DamanteAnne Lígia DokkedalRodrigo Cardoso de OliveiraABSTRACT The aim of this study was to evaluate the effect of ethanolic "aroeira" (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x103 cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium modified by Dulbecco - DMEM) supplemented with 10% fetal bovine serum was replaced by DMEM with different ethanolic extract concentration (0, 0.1, 1, 10, 100, and 1000μg / mL). The fibroblast viability was analyzed after 48,72, and 96 h by the neutral red capture test and violet crystal. The "aroeira" extract, at high concentrations (100 and 1000 µg/mL) caused decrease in both cellular viability tests (p<0.05). However, dilutions between 0.1 and 10 µg/mL did not affect the viability of the cells. It was concluded that "aroeira" extract was able to change the gingival fibroblast viability, and this effect was concentration dependent.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100301&lng=en&tlng=enMedicinal PlantfibroblastMyracrodruon urundeuva"aroeira""aroeira-do-sertão" |
| spellingShingle | Alessandra Cury Machado Patrícia Fernandes Sartori Carla Andreotti Damante Anne Lígia Dokkedal Rodrigo Cardoso de Oliveira Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão) Brazilian Archives of Biology and Technology Medicinal Plant fibroblast Myracrodruon urundeuva "aroeira" "aroeira-do-sertão" |
| title | Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão) |
| title_full | Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão) |
| title_fullStr | Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão) |
| title_full_unstemmed | Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão) |
| title_short | Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão) |
| title_sort | viability of human gingival fibroblast fgh treated with ethanolic aroeira extract myracrodruon urundeuva allemao |
| topic | Medicinal Plant fibroblast Myracrodruon urundeuva "aroeira" "aroeira-do-sertão" |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100301&lng=en&tlng=en |
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