Exosomal microRNA let-7-5p from <i>Taenia pisiformis</i> Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δ
<i>Cysticercus pisiformis</i>, the larval stage of <i>Taenia pisiformis</i>, causes serious illness in rabbits that severely impacts the rabbit breeding industry. An inhibitive Th2 immune response can be induced by let-7-enriched exosomes derived from <i>T. pisiformis&l...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2076-2607/9/7/1403 |
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| author | Liqun Wang Tingli Liu Guoliang Chen Yanping Li Shaohua Zhang Li Mao Panhong Liang Majid Fasihi Harandi Taoshan Li Xuenong Luo |
| author_facet | Liqun Wang Tingli Liu Guoliang Chen Yanping Li Shaohua Zhang Li Mao Panhong Liang Majid Fasihi Harandi Taoshan Li Xuenong Luo |
| author_sort | Liqun Wang |
| collection | DOAJ |
| description | <i>Cysticercus pisiformis</i>, the larval stage of <i>Taenia pisiformis</i>, causes serious illness in rabbits that severely impacts the rabbit breeding industry. An inhibitive Th2 immune response can be induced by let-7-enriched exosomes derived from <i>T. pisiformis</i> cysticercus. However, the underlying molecular mechanisms are not completely understood. Here, we report that exosomal miR-let-7-5p released by <i>T. pisiformis</i> cysticercus played a critical role in the activation of M2 macrophages. We found that overexpression of let-7-5p in M1 macrophages decreased M1 phenotype expression while promoting polarization to the M2 phenotype, which is consistent with experimental data in exosome-treated macrophages alone. In contrast, knockdown of let-7-5p in exosome-like vesicles promoted M1 polarization and decreased M2 phenotype expression. Furthermore, down-regulation of transcription factor CCAAT/enhancer-binding protein (C/EBP)-δ resulted in the decrease of M1 phenotype markers and increase of M2 phenotype markers. These results suggested that let-7 enriched in exosome-like vesicles from <i>T. pisiformis</i> metacestodes can induce M2 macrophage polarization via targeting C/EBP δ, which may be involved in macrophage polarization induced by <i>T. pisiformis</i> metacestodes. The finding helps to expand our knowledge of the molecular mechanism of immunosuppression and Th2 immune response induced by metacestodes. |
| first_indexed | 2024-03-10T09:57:55Z |
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| language | English |
| last_indexed | 2024-03-10T09:57:55Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-92513b16d2854cd984e8b58cb37f29252023-11-22T02:11:36ZengMDPI AGMicroorganisms2076-26072021-06-0197140310.3390/microorganisms9071403Exosomal microRNA let-7-5p from <i>Taenia pisiformis</i> Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δLiqun Wang0Tingli Liu1Guoliang Chen2Yanping Li3Shaohua Zhang4Li Mao5Panhong Liang6Majid Fasihi Harandi7Taoshan Li8Xuenong Luo9State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, ChinaResearch Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman 7616914115, IranState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, China<i>Cysticercus pisiformis</i>, the larval stage of <i>Taenia pisiformis</i>, causes serious illness in rabbits that severely impacts the rabbit breeding industry. An inhibitive Th2 immune response can be induced by let-7-enriched exosomes derived from <i>T. pisiformis</i> cysticercus. However, the underlying molecular mechanisms are not completely understood. Here, we report that exosomal miR-let-7-5p released by <i>T. pisiformis</i> cysticercus played a critical role in the activation of M2 macrophages. We found that overexpression of let-7-5p in M1 macrophages decreased M1 phenotype expression while promoting polarization to the M2 phenotype, which is consistent with experimental data in exosome-treated macrophages alone. In contrast, knockdown of let-7-5p in exosome-like vesicles promoted M1 polarization and decreased M2 phenotype expression. Furthermore, down-regulation of transcription factor CCAAT/enhancer-binding protein (C/EBP)-δ resulted in the decrease of M1 phenotype markers and increase of M2 phenotype markers. These results suggested that let-7 enriched in exosome-like vesicles from <i>T. pisiformis</i> metacestodes can induce M2 macrophage polarization via targeting C/EBP δ, which may be involved in macrophage polarization induced by <i>T. pisiformis</i> metacestodes. The finding helps to expand our knowledge of the molecular mechanism of immunosuppression and Th2 immune response induced by metacestodes.https://www.mdpi.com/2076-2607/9/7/1403<i>Taenia pisiformis</i> cysticercusexosomemiRNA let-7-5pmacrophages polarization |
| spellingShingle | Liqun Wang Tingli Liu Guoliang Chen Yanping Li Shaohua Zhang Li Mao Panhong Liang Majid Fasihi Harandi Taoshan Li Xuenong Luo Exosomal microRNA let-7-5p from <i>Taenia pisiformis</i> Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δ Microorganisms <i>Taenia pisiformis</i> cysticercus exosome miRNA let-7-5p macrophages polarization |
| title | Exosomal microRNA let-7-5p from <i>Taenia pisiformis</i> Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δ |
| title_full | Exosomal microRNA let-7-5p from <i>Taenia pisiformis</i> Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δ |
| title_fullStr | Exosomal microRNA let-7-5p from <i>Taenia pisiformis</i> Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δ |
| title_full_unstemmed | Exosomal microRNA let-7-5p from <i>Taenia pisiformis</i> Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δ |
| title_short | Exosomal microRNA let-7-5p from <i>Taenia pisiformis</i> Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δ |
| title_sort | exosomal microrna let 7 5p from i taenia pisiformis i cysticercus prompted macrophage to m2 polarization through inhibiting the expression of c ebp δ |
| topic | <i>Taenia pisiformis</i> cysticercus exosome miRNA let-7-5p macrophages polarization |
| url | https://www.mdpi.com/2076-2607/9/7/1403 |
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