Antimicrobial activity and comparative metabolomic analysis of Priestia megaterium strains derived from potato and dendrobium
Abstract The growth of endophytic bacteria is influenced by the host plants and their secondary metabolites and activities. In this study, P. megaterium P-NA14 and P. megaterium D-HT207 were isolated from potato tuber and dendrobium stem respectively. They were both identified as Priestia megaterium...
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| Format: | Article |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-32337-6 |
| _version_ | 1827974649936347136 |
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| author | Jia-Meng Liu Yan-Tian Liang Shan-Shan Wang Nuo Jin Jing Sun Cong Lu Yu-Feng Sun Shu-Ying Li Bei Fan Feng-Zhong Wang |
| author_facet | Jia-Meng Liu Yan-Tian Liang Shan-Shan Wang Nuo Jin Jing Sun Cong Lu Yu-Feng Sun Shu-Ying Li Bei Fan Feng-Zhong Wang |
| author_sort | Jia-Meng Liu |
| collection | DOAJ |
| description | Abstract The growth of endophytic bacteria is influenced by the host plants and their secondary metabolites and activities. In this study, P. megaterium P-NA14 and P. megaterium D-HT207 were isolated from potato tuber and dendrobium stem respectively. They were both identified as Priestia megaterium. The antimicrobial activities and metabolites of both strains were explored. For antimicrobial activities, results showed that P. megaterium P-NA14 exhibited a stronger inhibition effect on the pathogen of dendrobium, while P. megaterium D-HT207 exhibited a stronger inhibition effect on the pathogen of potato. The supernatant of P. megaterium P-NA14 showed an inhibition effect only on Staphylococcus aureus, while the sediment of P. megaterium D-HT207 showed an inhibition effect only on Escherichia coli. For metabolomic analysis, the content of L-phenylalanine in P. megaterium P-NA14 was higher than that of P. megaterium D-HT207, and several key downstream metabolites of L-phenylalanine were associated with inhibition of S. aureus including tyrosine, capsaicin, etc. Therefore, we speculated that the different antimicrobial activities between P. megaterium P-NA14 and P. megaterium D-HT207 were possibly related to the content of L-phenylalanine and its metabolites. This study preliminarily explored why the same strains isolated from different hosts exhibit different activities from the perspective of metabolomics. |
| first_indexed | 2024-04-09T19:57:40Z |
| format | Article |
| id | doaj.art-9256f9ffc5d04ca8a3b506c99b019ae3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T19:57:40Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9256f9ffc5d04ca8a3b506c99b019ae32023-04-03T05:23:03ZengNature PortfolioScientific Reports2045-23222023-03-0113111110.1038/s41598-023-32337-6Antimicrobial activity and comparative metabolomic analysis of Priestia megaterium strains derived from potato and dendrobiumJia-Meng Liu0Yan-Tian Liang1Shan-Shan Wang2Nuo Jin3Jing Sun4Cong Lu5Yu-Feng Sun6Shu-Ying Li7Bei Fan8Feng-Zhong Wang9Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesCollege of Pharmacy, Hunan University of Traditional Chinese MedicineKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, China/Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesAbstract The growth of endophytic bacteria is influenced by the host plants and their secondary metabolites and activities. In this study, P. megaterium P-NA14 and P. megaterium D-HT207 were isolated from potato tuber and dendrobium stem respectively. They were both identified as Priestia megaterium. The antimicrobial activities and metabolites of both strains were explored. For antimicrobial activities, results showed that P. megaterium P-NA14 exhibited a stronger inhibition effect on the pathogen of dendrobium, while P. megaterium D-HT207 exhibited a stronger inhibition effect on the pathogen of potato. The supernatant of P. megaterium P-NA14 showed an inhibition effect only on Staphylococcus aureus, while the sediment of P. megaterium D-HT207 showed an inhibition effect only on Escherichia coli. For metabolomic analysis, the content of L-phenylalanine in P. megaterium P-NA14 was higher than that of P. megaterium D-HT207, and several key downstream metabolites of L-phenylalanine were associated with inhibition of S. aureus including tyrosine, capsaicin, etc. Therefore, we speculated that the different antimicrobial activities between P. megaterium P-NA14 and P. megaterium D-HT207 were possibly related to the content of L-phenylalanine and its metabolites. This study preliminarily explored why the same strains isolated from different hosts exhibit different activities from the perspective of metabolomics.https://doi.org/10.1038/s41598-023-32337-6 |
| spellingShingle | Jia-Meng Liu Yan-Tian Liang Shan-Shan Wang Nuo Jin Jing Sun Cong Lu Yu-Feng Sun Shu-Ying Li Bei Fan Feng-Zhong Wang Antimicrobial activity and comparative metabolomic analysis of Priestia megaterium strains derived from potato and dendrobium Scientific Reports |
| title | Antimicrobial activity and comparative metabolomic analysis of Priestia megaterium strains derived from potato and dendrobium |
| title_full | Antimicrobial activity and comparative metabolomic analysis of Priestia megaterium strains derived from potato and dendrobium |
| title_fullStr | Antimicrobial activity and comparative metabolomic analysis of Priestia megaterium strains derived from potato and dendrobium |
| title_full_unstemmed | Antimicrobial activity and comparative metabolomic analysis of Priestia megaterium strains derived from potato and dendrobium |
| title_short | Antimicrobial activity and comparative metabolomic analysis of Priestia megaterium strains derived from potato and dendrobium |
| title_sort | antimicrobial activity and comparative metabolomic analysis of priestia megaterium strains derived from potato and dendrobium |
| url | https://doi.org/10.1038/s41598-023-32337-6 |
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