Overexpression of TaLBD16-4D alters plant architecture and heading date in transgenic wheat
Lateral organ boundaries domain (LBD) proteins, a class of plant-specific transcription factors with a special domain of lateral organ boundaries (LOB), play essential roles in plant growth and development. However, there is little known about the functions of these genes in wheat to date. Our previ...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.911993/full |
| _version_ | 1798031882312482816 |
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| author | Huifang Wang Xiaofan Han Xiaofeng Fu Xinling Sun Hailong Chen Xirui Wei Shubin Cui Yiguo Liu Weiwei Guo Ximei Li Jiewen Xing Yumei Zhang |
| author_facet | Huifang Wang Xiaofan Han Xiaofeng Fu Xinling Sun Hailong Chen Xirui Wei Shubin Cui Yiguo Liu Weiwei Guo Ximei Li Jiewen Xing Yumei Zhang |
| author_sort | Huifang Wang |
| collection | DOAJ |
| description | Lateral organ boundaries domain (LBD) proteins, a class of plant-specific transcription factors with a special domain of lateral organ boundaries (LOB), play essential roles in plant growth and development. However, there is little known about the functions of these genes in wheat to date. Our previous study demonstrated that TaLBD16-4D is conducive to increasing lateral root number in wheat. In the present work, we further examined important agronomical traits of the aerial part of transgenic wheat overexpressing TaLBD16-4D. Interestingly, it was revealed that overexpressing TaLBD16-4D could lead to early heading and multiple alterations of plant architecture, including decreased plant height, increased flag leaf size and stem diameter, reduced spike length and tillering number, improved spike density and grain width, and decreased grain length. Moreover, auxin-responsive experiments demonstrated that the expression of TaLBD16-4D in wild-type (WT) wheat plants showed a significant upregulation through 2,4-D treatment. TaLBD16-4D-overexpression lines displayed a hyposensitivity to 2,4-D treatment and reduced shoot gravitropic response. The expressions of a set of auxin-responsive genes were markedly different between WT and transgenic plants. In addition, overexpressing TaLBD16-4D affected the transcript levels of flowering-related genes (TaGI, TaCO1, TaHd1, TaVRN1, TaVRN2, and TaFT1). Notably, the expression of TaGI, TaCO1, TaHd1, TaVRN1, and TaFT1 displayed significant upregulation under IAA treatment. Collectively, our observations indicated that overexpressing TaLBD16-4D could affect aerial architecture and heading time possibly though participating in the auxin pathway. |
| first_indexed | 2024-04-11T20:04:53Z |
| format | Article |
| id | doaj.art-925738bad2b04e909327e687f1de5bb0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T20:04:53Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-925738bad2b04e909327e687f1de5bb02022-12-22T04:05:23ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.911993911993Overexpression of TaLBD16-4D alters plant architecture and heading date in transgenic wheatHuifang Wang0Xiaofan Han1Xiaofeng Fu2Xinling Sun3Hailong Chen4Xirui Wei5Shubin Cui6Yiguo Liu7Weiwei Guo8Ximei Li9Jiewen Xing10Yumei Zhang11Shandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaShandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaShandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaShandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaShandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaShandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, ChinaShandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaShandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaShandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaState Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, ChinaShandong Provincial Key Laboratory of Dryland Farming Technology, Qingdao Agricultural University, Qingdao, ChinaLateral organ boundaries domain (LBD) proteins, a class of plant-specific transcription factors with a special domain of lateral organ boundaries (LOB), play essential roles in plant growth and development. However, there is little known about the functions of these genes in wheat to date. Our previous study demonstrated that TaLBD16-4D is conducive to increasing lateral root number in wheat. In the present work, we further examined important agronomical traits of the aerial part of transgenic wheat overexpressing TaLBD16-4D. Interestingly, it was revealed that overexpressing TaLBD16-4D could lead to early heading and multiple alterations of plant architecture, including decreased plant height, increased flag leaf size and stem diameter, reduced spike length and tillering number, improved spike density and grain width, and decreased grain length. Moreover, auxin-responsive experiments demonstrated that the expression of TaLBD16-4D in wild-type (WT) wheat plants showed a significant upregulation through 2,4-D treatment. TaLBD16-4D-overexpression lines displayed a hyposensitivity to 2,4-D treatment and reduced shoot gravitropic response. The expressions of a set of auxin-responsive genes were markedly different between WT and transgenic plants. In addition, overexpressing TaLBD16-4D affected the transcript levels of flowering-related genes (TaGI, TaCO1, TaHd1, TaVRN1, TaVRN2, and TaFT1). Notably, the expression of TaGI, TaCO1, TaHd1, TaVRN1, and TaFT1 displayed significant upregulation under IAA treatment. Collectively, our observations indicated that overexpressing TaLBD16-4D could affect aerial architecture and heading time possibly though participating in the auxin pathway.https://www.frontiersin.org/articles/10.3389/fpls.2022.911993/fullTaLBD16-4Dplant architectureheading timeauxinwheat |
| spellingShingle | Huifang Wang Xiaofan Han Xiaofeng Fu Xinling Sun Hailong Chen Xirui Wei Shubin Cui Yiguo Liu Weiwei Guo Ximei Li Jiewen Xing Yumei Zhang Overexpression of TaLBD16-4D alters plant architecture and heading date in transgenic wheat Frontiers in Plant Science TaLBD16-4D plant architecture heading time auxin wheat |
| title | Overexpression of TaLBD16-4D alters plant architecture and heading date in transgenic wheat |
| title_full | Overexpression of TaLBD16-4D alters plant architecture and heading date in transgenic wheat |
| title_fullStr | Overexpression of TaLBD16-4D alters plant architecture and heading date in transgenic wheat |
| title_full_unstemmed | Overexpression of TaLBD16-4D alters plant architecture and heading date in transgenic wheat |
| title_short | Overexpression of TaLBD16-4D alters plant architecture and heading date in transgenic wheat |
| title_sort | overexpression of talbd16 4d alters plant architecture and heading date in transgenic wheat |
| topic | TaLBD16-4D plant architecture heading time auxin wheat |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.911993/full |
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