A novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcus
In this artice, we are going to report results of a study in which with the help of layer-by-layer modification of a glassy carbon electrode (GCE), a novel biosensor was fabricated whose impedimetric and amperometric responses were correlated with the number of pneumococcus (P). The GCE was modified...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Sensing and Bio-Sensing Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180422000356 |
| _version_ | 1817999250663931904 |
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| author | Azam Yaghoobi Ramin Abiri Amirhoushang Alvandi Elham Arkan Ali R. Jalalvand |
| author_facet | Azam Yaghoobi Ramin Abiri Amirhoushang Alvandi Elham Arkan Ali R. Jalalvand |
| author_sort | Azam Yaghoobi |
| collection | DOAJ |
| description | In this artice, we are going to report results of a study in which with the help of layer-by-layer modification of a glassy carbon electrode (GCE), a novel biosensor was fabricated whose impedimetric and amperometric responses were correlated with the number of pneumococcus (P). The GCE was modified by graphene-multiwalled carbon nanotubes (Gr-MWCNTs)/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (CS-IL)/gold‑lead nanoparticles (AuPb NPs)/DNA-[FAM]CGC AAT CTA GCA GAT GAA GCA GGA AAA AAA AAA [Thiol] (DNA-Q-S). All the modification layers were characterized by microscopic and electrochemical techniques which confirmed the successfulness of all of the modification steps. The charge transfer resistance of impedimetric response and current of amperometric response of the biosensor were correlated with the number of the Ps which helped us to develop a novel biosensing system for sensing the Ps whose performance was comparable with a reference method. Therefore, the biosensor can be suggested for clinical purposes as a fast, stable, simple, sensitive, selective, repeatable and reproducible electronic device. |
| first_indexed | 2024-04-14T03:06:24Z |
| format | Article |
| id | doaj.art-92580086ee0547e9859ae80eeef8233b |
| institution | Directory Open Access Journal |
| issn | 2214-1804 |
| language | English |
| last_indexed | 2024-04-14T03:06:24Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj.art-92580086ee0547e9859ae80eeef8233b2022-12-22T02:15:44ZengElsevierSensing and Bio-Sensing Research2214-18042022-08-0137100506A novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcusAzam Yaghoobi0Ramin Abiri1Amirhoushang Alvandi2Elham Arkan3Ali R. Jalalvand4Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranNano Drug Delivery Research Center, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah, IranResearch Center of Oils and Fats, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran; Corresponding author.In this artice, we are going to report results of a study in which with the help of layer-by-layer modification of a glassy carbon electrode (GCE), a novel biosensor was fabricated whose impedimetric and amperometric responses were correlated with the number of pneumococcus (P). The GCE was modified by graphene-multiwalled carbon nanotubes (Gr-MWCNTs)/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (CS-IL)/gold‑lead nanoparticles (AuPb NPs)/DNA-[FAM]CGC AAT CTA GCA GAT GAA GCA GGA AAA AAA AAA [Thiol] (DNA-Q-S). All the modification layers were characterized by microscopic and electrochemical techniques which confirmed the successfulness of all of the modification steps. The charge transfer resistance of impedimetric response and current of amperometric response of the biosensor were correlated with the number of the Ps which helped us to develop a novel biosensing system for sensing the Ps whose performance was comparable with a reference method. Therefore, the biosensor can be suggested for clinical purposes as a fast, stable, simple, sensitive, selective, repeatable and reproducible electronic device.http://www.sciencedirect.com/science/article/pii/S2214180422000356PneumococcusBiosensorImpedimetryAmperometry |
| spellingShingle | Azam Yaghoobi Ramin Abiri Amirhoushang Alvandi Elham Arkan Ali R. Jalalvand A novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcus Sensing and Bio-Sensing Research Pneumococcus Biosensor Impedimetry Amperometry |
| title | A novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcus |
| title_full | A novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcus |
| title_fullStr | A novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcus |
| title_full_unstemmed | A novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcus |
| title_short | A novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcus |
| title_sort | novel electrochemical biosensor as an efficient electronic device for impedimetric and amperometric quantification of the pneumococcus |
| topic | Pneumococcus Biosensor Impedimetry Amperometry |
| url | http://www.sciencedirect.com/science/article/pii/S2214180422000356 |
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