<b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841
Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA)...
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Format: | Article |
Language: | English |
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Eduem (Editora da Universidade Estadual de Maringá)
2010-04-01
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Series: | Acta Scientiarum: Agronomy |
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Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841 |
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author | Viviane Cristina Vieira Pedro Luís da Costa Aguiar Alves Simone Cristina Picchi Manoel Victor Franco Lemos Janete Apparecida Desidério Sena |
author_facet | Viviane Cristina Vieira Pedro Luís da Costa Aguiar Alves Simone Cristina Picchi Manoel Victor Franco Lemos Janete Apparecida Desidério Sena |
author_sort | Viviane Cristina Vieira |
collection | DOAJ |
description | Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn. |
first_indexed | 2024-12-20T15:26:52Z |
format | Article |
id | doaj.art-92598aa42aa545b593cbe5a3f78b4e9d |
institution | Directory Open Access Journal |
issn | 1679-9275 1807-8621 |
language | English |
last_indexed | 2024-12-20T15:26:52Z |
publishDate | 2010-04-01 |
publisher | Eduem (Editora da Universidade Estadual de Maringá) |
record_format | Article |
series | Acta Scientiarum: Agronomy |
spelling | doaj.art-92598aa42aa545b593cbe5a3f78b4e9d2022-12-21T19:35:48ZengEduem (Editora da Universidade Estadual de Maringá)Acta Scientiarum: Agronomy1679-92751807-86212010-04-0132210.4025/actasciagron.v32i2.3841<b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841Viviane Cristina Vieira0Pedro Luís da Costa Aguiar Alves1Simone Cristina Picchi2Manoel Victor Franco Lemos3Janete Apparecida Desidério Sena4Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaFaculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaFaculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaFaculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaFaculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaDigitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.https://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841DigitariapsbA geneRAPDsequencingtriazineweed |
spellingShingle | Viviane Cristina Vieira Pedro Luís da Costa Aguiar Alves Simone Cristina Picchi Manoel Victor Franco Lemos Janete Apparecida Desidério Sena <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841 Acta Scientiarum: Agronomy Digitaria psbA gene RAPD sequencing triazine weed |
title | <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841 |
title_full | <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841 |
title_fullStr | <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841 |
title_full_unstemmed | <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841 |
title_short | <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841 |
title_sort | b molecular characterization of accessions of crabgrass em digitaria nuda em and response to ametryn b doi 10 4025 actasciagron v32i2 3841 |
topic | Digitaria psbA gene RAPD sequencing triazine weed |
url | https://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841 |
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