<b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841

Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA)...

Full description

Bibliographic Details
Main Authors: Viviane Cristina Vieira, Pedro Luís da Costa Aguiar Alves, Simone Cristina Picchi, Manoel Victor Franco Lemos, Janete Apparecida Desidério Sena
Format: Article
Language:English
Published: Eduem (Editora da Universidade Estadual de Maringá) 2010-04-01
Series:Acta Scientiarum: Agronomy
Subjects:
Online Access:https://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841
_version_ 1818973608053047296
author Viviane Cristina Vieira
Pedro Luís da Costa Aguiar Alves
Simone Cristina Picchi
Manoel Victor Franco Lemos
Janete Apparecida Desidério Sena
author_facet Viviane Cristina Vieira
Pedro Luís da Costa Aguiar Alves
Simone Cristina Picchi
Manoel Victor Franco Lemos
Janete Apparecida Desidério Sena
author_sort Viviane Cristina Vieira
collection DOAJ
description Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.
first_indexed 2024-12-20T15:26:52Z
format Article
id doaj.art-92598aa42aa545b593cbe5a3f78b4e9d
institution Directory Open Access Journal
issn 1679-9275
1807-8621
language English
last_indexed 2024-12-20T15:26:52Z
publishDate 2010-04-01
publisher Eduem (Editora da Universidade Estadual de Maringá)
record_format Article
series Acta Scientiarum: Agronomy
spelling doaj.art-92598aa42aa545b593cbe5a3f78b4e9d2022-12-21T19:35:48ZengEduem (Editora da Universidade Estadual de Maringá)Acta Scientiarum: Agronomy1679-92751807-86212010-04-0132210.4025/actasciagron.v32i2.3841<b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841Viviane Cristina Vieira0Pedro Luís da Costa Aguiar Alves1Simone Cristina Picchi2Manoel Victor Franco Lemos3Janete Apparecida Desidério Sena4Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaFaculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaFaculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaFaculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaFaculdade de Ciências Agrárias e Veterinárias/Universidade Estadual PaulistaDigitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.https://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841DigitariapsbA geneRAPDsequencingtriazineweed
spellingShingle Viviane Cristina Vieira
Pedro Luís da Costa Aguiar Alves
Simone Cristina Picchi
Manoel Victor Franco Lemos
Janete Apparecida Desidério Sena
<b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841
Acta Scientiarum: Agronomy
Digitaria
psbA gene
RAPD
sequencing
triazine
weed
title <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841
title_full <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841
title_fullStr <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841
title_full_unstemmed <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841
title_short <b>Molecular characterization of accessions of crabgrass (<em>Digitaria nuda</em>) and response to ametryn</b> - DOI: 10.4025/actasciagron.v32i2.3841
title_sort b molecular characterization of accessions of crabgrass em digitaria nuda em and response to ametryn b doi 10 4025 actasciagron v32i2 3841
topic Digitaria
psbA gene
RAPD
sequencing
triazine
weed
url https://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841
work_keys_str_mv AT vivianecristinavieira bmolecularcharacterizationofaccessionsofcrabgrassemdigitarianudaemandresponsetoametrynbdoi104025actasciagronv32i23841
AT pedroluisdacostaaguiaralves bmolecularcharacterizationofaccessionsofcrabgrassemdigitarianudaemandresponsetoametrynbdoi104025actasciagronv32i23841
AT simonecristinapicchi bmolecularcharacterizationofaccessionsofcrabgrassemdigitarianudaemandresponsetoametrynbdoi104025actasciagronv32i23841
AT manoelvictorfrancolemos bmolecularcharacterizationofaccessionsofcrabgrassemdigitarianudaemandresponsetoametrynbdoi104025actasciagronv32i23841
AT janeteapparecidadesideriosena bmolecularcharacterizationofaccessionsofcrabgrassemdigitarianudaemandresponsetoametrynbdoi104025actasciagronv32i23841