The Dynamics of miR-449a/c Expression during Uterine Cycles Are Associated with Endometrial Development
The human endometrium is a highly dynamic tissue. Increasing evidence has shown that microRNAs (miRs) play essential roles in human endometrium development. Our previous assay, based on small RNA-sequencing (sRNA-seq) indicated the complexity and dynamics of numerous sequence variants of miRs (isomi...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2079-7737/12/1/55 |
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| author | Mladen Naydenov Maria Nikolova Apostol Apostolov Ilias Glogovitis Andres Salumets Vesselin Baev Galina Yahubyan |
| author_facet | Mladen Naydenov Maria Nikolova Apostol Apostolov Ilias Glogovitis Andres Salumets Vesselin Baev Galina Yahubyan |
| author_sort | Mladen Naydenov |
| collection | DOAJ |
| description | The human endometrium is a highly dynamic tissue. Increasing evidence has shown that microRNAs (miRs) play essential roles in human endometrium development. Our previous assay, based on small RNA-sequencing (sRNA-seq) indicated the complexity and dynamics of numerous sequence variants of miRs (isomiRs) that can act together to control genes of functional relevance to the receptive endometrium (RE). Here, we used a greater average depth of sRNA-seq to detect poorly expressed small RNAs. The sequencing data confirmed the up-regulation of miR-449c and uncovered other members of the miR-449 family up-regulated in RE—among them miR-449a, as well as several isoforms of both miR-449a and miR-449c, while the third family member, miR-449b, was not identified. Stem-looped RT-qPCR analysis of miR expression at four-time points of the endometrial cycle verified the increased expression of the miR-449a/c family members in RE, among which the 5′ isoform of miR-449c–miR-449c.1 was the most strongly up-regulated. Moreover, we found in a case study that the expression of miR-449c.1 and its precursor correlated with the histological assessment of the endometrial phase and patient age. We believe this study will promote the clinical investigation and application of the miR-449 family in the diagnosis and prognosis of human reproductive diseases. |
| first_indexed | 2024-03-09T13:32:15Z |
| format | Article |
| id | doaj.art-925de122317e4ee9bdcdcf6f47ecd94b |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T13:32:15Z |
| publishDate | 2022-12-01 |
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| series | Biology |
| spelling | doaj.art-925de122317e4ee9bdcdcf6f47ecd94b2023-11-30T21:16:43ZengMDPI AGBiology2079-77372022-12-011215510.3390/biology12010055The Dynamics of miR-449a/c Expression during Uterine Cycles Are Associated with Endometrial DevelopmentMladen Naydenov0Maria Nikolova1Apostol Apostolov2Ilias Glogovitis3Andres Salumets4Vesselin Baev5Galina Yahubyan6Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaFaculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaFaculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaFaculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaCompetence Centre on Health Technologies, 50406 Tartu, EstoniaFaculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaFaculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaThe human endometrium is a highly dynamic tissue. Increasing evidence has shown that microRNAs (miRs) play essential roles in human endometrium development. Our previous assay, based on small RNA-sequencing (sRNA-seq) indicated the complexity and dynamics of numerous sequence variants of miRs (isomiRs) that can act together to control genes of functional relevance to the receptive endometrium (RE). Here, we used a greater average depth of sRNA-seq to detect poorly expressed small RNAs. The sequencing data confirmed the up-regulation of miR-449c and uncovered other members of the miR-449 family up-regulated in RE—among them miR-449a, as well as several isoforms of both miR-449a and miR-449c, while the third family member, miR-449b, was not identified. Stem-looped RT-qPCR analysis of miR expression at four-time points of the endometrial cycle verified the increased expression of the miR-449a/c family members in RE, among which the 5′ isoform of miR-449c–miR-449c.1 was the most strongly up-regulated. Moreover, we found in a case study that the expression of miR-449c.1 and its precursor correlated with the histological assessment of the endometrial phase and patient age. We believe this study will promote the clinical investigation and application of the miR-449 family in the diagnosis and prognosis of human reproductive diseases.https://www.mdpi.com/2079-7737/12/1/55microRNAsisomiRsmiR-449 familyendometrial receptivity |
| spellingShingle | Mladen Naydenov Maria Nikolova Apostol Apostolov Ilias Glogovitis Andres Salumets Vesselin Baev Galina Yahubyan The Dynamics of miR-449a/c Expression during Uterine Cycles Are Associated with Endometrial Development Biology microRNAs isomiRs miR-449 family endometrial receptivity |
| title | The Dynamics of miR-449a/c Expression during Uterine Cycles Are Associated with Endometrial Development |
| title_full | The Dynamics of miR-449a/c Expression during Uterine Cycles Are Associated with Endometrial Development |
| title_fullStr | The Dynamics of miR-449a/c Expression during Uterine Cycles Are Associated with Endometrial Development |
| title_full_unstemmed | The Dynamics of miR-449a/c Expression during Uterine Cycles Are Associated with Endometrial Development |
| title_short | The Dynamics of miR-449a/c Expression during Uterine Cycles Are Associated with Endometrial Development |
| title_sort | dynamics of mir 449a c expression during uterine cycles are associated with endometrial development |
| topic | microRNAs isomiRs miR-449 family endometrial receptivity |
| url | https://www.mdpi.com/2079-7737/12/1/55 |
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