The volatilome reveals microcystin concentration, microbial composition, and oxidative stress in a critical Oregon freshwater lake
ABSTRACT Toxins produced by cyanobacterial blooms in freshwater lakes are a serious public health problem. The conditions leading to toxin production are unpredictable, thereby requiring expensive sampling and monitoring programs globally. We explored the potential of volatile organic compounds (VOC...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-10-01
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| Series: | mSystems |
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| Online Access: | https://journals.asm.org/doi/10.1128/msystems.00379-23 |
| _version_ | 1797617654382460928 |
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| author | Lindsay Collart Duo Jiang Kimberly H. Halsey |
| author_facet | Lindsay Collart Duo Jiang Kimberly H. Halsey |
| author_sort | Lindsay Collart |
| collection | DOAJ |
| description | ABSTRACT Toxins produced by cyanobacterial blooms in freshwater lakes are a serious public health problem. The conditions leading to toxin production are unpredictable, thereby requiring expensive sampling and monitoring programs globally. We explored the potential of volatile organic compounds (VOCs) to indicate microcystin presence and concentration and microbial community composition in Upper Klamath Lake, Oregon. Elastic net regularization regression selected 24 of 229 detected m/z + 1 values (corresponding to unique VOCs) in models predicting microcystin contamination. These models outperformed regression models based only on environmental parameters, including chlorophyll, pH, and temperature. Several m/z + 1 values selected by elastic net were putatively identified as saturated fatty aldehydes, which defend cyanobacteria against oxidative stress. Elastic net also identified unique sets of m/z + 1 values that predicted the relative abundance of the dominant bacterial phyla, classes, and cyanobacterial genera. VOCs appear to reveal the physiological status of cyanobacteria during toxic blooms and may be a key component of lake monitoring strategies. IMPORTANCE Harmful algal blooms are among the most significant threats to drinking water safety. Blooms dominated by cyanobacteria can produce potentially harmful toxins and, despite intensive research, toxin production remains unpredictable. We measured gaseous molecules in Upper Klamath Lake, Oregon, over 2 years and used them to predict the presence and concentration of the cyanotoxin, microcystin, and microbial community composition. Subsets of gaseous compounds were identified that are associated with microcystin production during oxidative stress, pointing to ecosystem-level interactions leading to microcystin contamination. Our approach shows potential for gaseous molecules to be harnessed in monitoring critical waterways. |
| first_indexed | 2024-03-11T07:58:55Z |
| format | Article |
| id | doaj.art-926472c127934ee0a748dba404fec0b7 |
| institution | Directory Open Access Journal |
| issn | 2379-5077 |
| language | English |
| last_indexed | 2024-03-11T07:58:55Z |
| publishDate | 2023-10-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSystems |
| spelling | doaj.art-926472c127934ee0a748dba404fec0b72023-11-17T03:22:37ZengAmerican Society for MicrobiologymSystems2379-50772023-10-018510.1128/msystems.00379-23The volatilome reveals microcystin concentration, microbial composition, and oxidative stress in a critical Oregon freshwater lakeLindsay Collart0Duo Jiang1Kimberly H. Halsey2Department of Microbiology, Oregon State University , Corvallis, Oregon, USADepartment of Statistics, Oregon State University , Corvallis, Oregon, USADepartment of Microbiology, Oregon State University , Corvallis, Oregon, USAABSTRACT Toxins produced by cyanobacterial blooms in freshwater lakes are a serious public health problem. The conditions leading to toxin production are unpredictable, thereby requiring expensive sampling and monitoring programs globally. We explored the potential of volatile organic compounds (VOCs) to indicate microcystin presence and concentration and microbial community composition in Upper Klamath Lake, Oregon. Elastic net regularization regression selected 24 of 229 detected m/z + 1 values (corresponding to unique VOCs) in models predicting microcystin contamination. These models outperformed regression models based only on environmental parameters, including chlorophyll, pH, and temperature. Several m/z + 1 values selected by elastic net were putatively identified as saturated fatty aldehydes, which defend cyanobacteria against oxidative stress. Elastic net also identified unique sets of m/z + 1 values that predicted the relative abundance of the dominant bacterial phyla, classes, and cyanobacterial genera. VOCs appear to reveal the physiological status of cyanobacteria during toxic blooms and may be a key component of lake monitoring strategies. IMPORTANCE Harmful algal blooms are among the most significant threats to drinking water safety. Blooms dominated by cyanobacteria can produce potentially harmful toxins and, despite intensive research, toxin production remains unpredictable. We measured gaseous molecules in Upper Klamath Lake, Oregon, over 2 years and used them to predict the presence and concentration of the cyanotoxin, microcystin, and microbial community composition. Subsets of gaseous compounds were identified that are associated with microcystin production during oxidative stress, pointing to ecosystem-level interactions leading to microcystin contamination. Our approach shows potential for gaseous molecules to be harnessed in monitoring critical waterways.https://journals.asm.org/doi/10.1128/msystems.00379-23cyanobacteriavolatile organic compoundsmicrocystinharmful algal bloomscyanotoxinssaturated fatty aldehydes |
| spellingShingle | Lindsay Collart Duo Jiang Kimberly H. Halsey The volatilome reveals microcystin concentration, microbial composition, and oxidative stress in a critical Oregon freshwater lake mSystems cyanobacteria volatile organic compounds microcystin harmful algal blooms cyanotoxins saturated fatty aldehydes |
| title | The volatilome reveals microcystin concentration, microbial composition, and oxidative stress in a critical Oregon freshwater lake |
| title_full | The volatilome reveals microcystin concentration, microbial composition, and oxidative stress in a critical Oregon freshwater lake |
| title_fullStr | The volatilome reveals microcystin concentration, microbial composition, and oxidative stress in a critical Oregon freshwater lake |
| title_full_unstemmed | The volatilome reveals microcystin concentration, microbial composition, and oxidative stress in a critical Oregon freshwater lake |
| title_short | The volatilome reveals microcystin concentration, microbial composition, and oxidative stress in a critical Oregon freshwater lake |
| title_sort | volatilome reveals microcystin concentration microbial composition and oxidative stress in a critical oregon freshwater lake |
| topic | cyanobacteria volatile organic compounds microcystin harmful algal blooms cyanotoxins saturated fatty aldehydes |
| url | https://journals.asm.org/doi/10.1128/msystems.00379-23 |
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