Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV)
There is a growing diversity of bat-associated lyssaviruses in the Old World. In August 2017, a dead Brandt’s bat (<i>Myotis brandtii</i>) tested positive for rabies and based on partial sequence analysis, the novel Kotalahti bat lyssavirus (KBLV) was identified. Because the bat was in a...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/1/69 |
| _version_ | 1797415776911622144 |
|---|---|
| author | Sten Calvelage Niina Tammiranta Tiina Nokireki Tuija Gadd Elisa Eggerbauer Luca M. Zaeck Madlin Potratz Claudia Wylezich Dirk Höper Thomas Müller Stefan Finke Conrad M. Freuling |
| author_facet | Sten Calvelage Niina Tammiranta Tiina Nokireki Tuija Gadd Elisa Eggerbauer Luca M. Zaeck Madlin Potratz Claudia Wylezich Dirk Höper Thomas Müller Stefan Finke Conrad M. Freuling |
| author_sort | Sten Calvelage |
| collection | DOAJ |
| description | There is a growing diversity of bat-associated lyssaviruses in the Old World. In August 2017, a dead Brandt’s bat (<i>Myotis brandtii</i>) tested positive for rabies and based on partial sequence analysis, the novel Kotalahti bat lyssavirus (KBLV) was identified. Because the bat was in an autolyzed state, isolation of KBLV was neither successful after three consecutive cell passages on cells nor in mice. Next generation sequencing (NGS) was applied using Ion Torrent ™ S5 technology coupled with target enrichment via hybridization-based capture (myBaits<sup>®</sup>) was used to sequence 99% of the genome, comprising of 11,878 nucleotides (nt). KBLV is most closely related to EBLV-2 (78.7% identity), followed by KHUV (79.0%) and BBLV (77.6%), supporting the assignment as phylogroup I lyssavirus. Interestingly, all of these lyssaviruses were also isolated from bat species of the genus Myotis, thus supporting that <i>M. brandtii</i> is likely the reservoir host. All information on antigenic and genetic divergence fulfil the species demarcation criteria by ICTV, so that we recommend KBLV as a novel species within the Lyssavirus genus. Next to sequence analyses, assignment to phylogroup I was functionally corroborated by cross-neutralization of G-deleted RABV, pseudotyped with KBLV-G by sera from RABV vaccinated humans. This suggests that conventional RABV vaccines also confer protection against the novel KBLV. |
| first_indexed | 2024-03-09T05:54:26Z |
| format | Article |
| id | doaj.art-9265228825fa4a6ab95f52f20689918b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T05:54:26Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-9265228825fa4a6ab95f52f20689918b2023-12-03T12:14:54ZengMDPI AGViruses1999-49152021-01-011316910.3390/v13010069Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV)Sten Calvelage0Niina Tammiranta1Tiina Nokireki2Tuija Gadd3Elisa Eggerbauer4Luca M. Zaeck5Madlin Potratz6Claudia Wylezich7Dirk Höper8Thomas Müller9Stefan Finke10Conrad M. Freuling11Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, GermanyFinnish Food Authority, Research Department, Virology Unit, Mustialankatu 3, FI-00790 Helsinki, FinlandFinnish Food Authority, Research Department, Virology Unit, Mustialankatu 3, FI-00790 Helsinki, FinlandFinnish Food Authority, Research Department, Virology Unit, Mustialankatu 3, FI-00790 Helsinki, FinlandInstitute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut (FLI), WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, 17493 Greifswald-Insel Riems, GermanyInstitute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut (FLI), WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, 17493 Greifswald-Insel Riems, GermanyInstitute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut (FLI), WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, 17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, GermanyInstitute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut (FLI), WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, 17493 Greifswald-Insel Riems, GermanyInstitute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut (FLI), WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, 17493 Greifswald-Insel Riems, GermanyCentral Duties, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, GermanyThere is a growing diversity of bat-associated lyssaviruses in the Old World. In August 2017, a dead Brandt’s bat (<i>Myotis brandtii</i>) tested positive for rabies and based on partial sequence analysis, the novel Kotalahti bat lyssavirus (KBLV) was identified. Because the bat was in an autolyzed state, isolation of KBLV was neither successful after three consecutive cell passages on cells nor in mice. Next generation sequencing (NGS) was applied using Ion Torrent ™ S5 technology coupled with target enrichment via hybridization-based capture (myBaits<sup>®</sup>) was used to sequence 99% of the genome, comprising of 11,878 nucleotides (nt). KBLV is most closely related to EBLV-2 (78.7% identity), followed by KHUV (79.0%) and BBLV (77.6%), supporting the assignment as phylogroup I lyssavirus. Interestingly, all of these lyssaviruses were also isolated from bat species of the genus Myotis, thus supporting that <i>M. brandtii</i> is likely the reservoir host. All information on antigenic and genetic divergence fulfil the species demarcation criteria by ICTV, so that we recommend KBLV as a novel species within the Lyssavirus genus. Next to sequence analyses, assignment to phylogroup I was functionally corroborated by cross-neutralization of G-deleted RABV, pseudotyped with KBLV-G by sera from RABV vaccinated humans. This suggests that conventional RABV vaccines also confer protection against the novel KBLV.https://www.mdpi.com/1999-4915/13/1/69KBLVrabies<i>Myotis brandtii</i>lyssavirus |
| spellingShingle | Sten Calvelage Niina Tammiranta Tiina Nokireki Tuija Gadd Elisa Eggerbauer Luca M. Zaeck Madlin Potratz Claudia Wylezich Dirk Höper Thomas Müller Stefan Finke Conrad M. Freuling Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV) Viruses KBLV rabies <i>Myotis brandtii</i> lyssavirus |
| title | Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV) |
| title_full | Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV) |
| title_fullStr | Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV) |
| title_full_unstemmed | Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV) |
| title_short | Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV) |
| title_sort | genetic and antigenetic characterization of the novel kotalahti bat lyssavirus kblv |
| topic | KBLV rabies <i>Myotis brandtii</i> lyssavirus |
| url | https://www.mdpi.com/1999-4915/13/1/69 |
| work_keys_str_mv | AT stencalvelage geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT niinatammiranta geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT tiinanokireki geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT tuijagadd geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT elisaeggerbauer geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT lucamzaeck geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT madlinpotratz geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT claudiawylezich geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT dirkhoper geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT thomasmuller geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT stefanfinke geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv AT conradmfreuling geneticandantigeneticcharacterizationofthenovelkotalahtibatlyssaviruskblv |