Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layer
Background: Cross talk of tumor–immune cells at the gene expression level has been an area of intense research. However, it is largely unknown at the alternative splicing level which has been found to play important roles in the tumor–immune microenvironment. Results: Here, we re-exploited one trans...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345821000014 |
| _version_ | 1818676832625491968 |
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| author | Lina Zhao Xi Yang Chun Feng Yue Wang Qing Wang Jiahong Pei Jinting Wu Shuaiying Li Honglei Zhang Xianbao Cao |
| author_facet | Lina Zhao Xi Yang Chun Feng Yue Wang Qing Wang Jiahong Pei Jinting Wu Shuaiying Li Honglei Zhang Xianbao Cao |
| author_sort | Lina Zhao |
| collection | DOAJ |
| description | Background: Cross talk of tumor–immune cells at the gene expression level has been an area of intense research. However, it is largely unknown at the alternative splicing level which has been found to play important roles in the tumor–immune microenvironment. Results: Here, we re-exploited one transcriptomic dataset to gain insight into tumor–immune interactions from the point of AS level. Our results showed that the AS profiles of triple-negative breast cancer cells co-cultured with activated T cells were significantly changed but not Estrogen receptor positive cells. We further suggested that the alteration in AS profiles in triple-negative breast cancer cells was largely caused by activated T cells rather than paracrine factors from activated T cells. Biological pathway analyses showed that translation initiation and tRNA aminoacylation pathways were most disturbed with T cell treatment. We also established an approach largely based on the AS factor–AS events associations and identified LSM7, an alternative splicing factor, may be responsible for the major altered events. Conclusions: Our study reveals the notable differences of response to T cells among breast cancer types which may facilitate the development or improvement of tumor immunotherapy.How to cite: Zhao L, Yang X, Feng C, et al. Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layer. Electron J Biotechnol 2021;50.https://doi.org/10.1016/j.ejbt.2021.01.001 |
| first_indexed | 2024-12-17T08:49:45Z |
| format | Article |
| id | doaj.art-9268d25885d847f082589e6fcf690877 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-17T08:49:45Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-9268d25885d847f082589e6fcf6908772022-12-21T21:56:06ZengElsevierElectronic Journal of Biotechnology0717-34582021-03-01505967Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layerLina Zhao0Xi Yang1Chun Feng2Yue Wang3Qing Wang4Jiahong Pei5Jinting Wu6Shuaiying Li7Honglei Zhang8Xianbao Cao9Department of Otolaryngology, the First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, China; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China; Kunming College of Life Science, University of Chinese Academy of Sciences, 650223 Kunming, ChinaThe Second Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650223, ChinaDepartment of Otolaryngology, the First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, ChinaDepartment of Breast Cancer, Third Affiliated Hospital of Kunming Medical University, Kunming 650118, ChinaDepartment of Oncology, Qujing First People’s Hospital, Qujing 202150, ChinaDepartment of Otolaryngology, the First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, ChinaYunnan University of Chinese Medicine, Kunming 650500, ChinaYunnan University of Chinese Medicine, Kunming 650500, ChinaState Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China; Yunnan University of Chinese Medicine, Kunming 650500, China; Corresponding authors.Department of Otolaryngology, the First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, China; Corresponding authors.Background: Cross talk of tumor–immune cells at the gene expression level has been an area of intense research. However, it is largely unknown at the alternative splicing level which has been found to play important roles in the tumor–immune microenvironment. Results: Here, we re-exploited one transcriptomic dataset to gain insight into tumor–immune interactions from the point of AS level. Our results showed that the AS profiles of triple-negative breast cancer cells co-cultured with activated T cells were significantly changed but not Estrogen receptor positive cells. We further suggested that the alteration in AS profiles in triple-negative breast cancer cells was largely caused by activated T cells rather than paracrine factors from activated T cells. Biological pathway analyses showed that translation initiation and tRNA aminoacylation pathways were most disturbed with T cell treatment. We also established an approach largely based on the AS factor–AS events associations and identified LSM7, an alternative splicing factor, may be responsible for the major altered events. Conclusions: Our study reveals the notable differences of response to T cells among breast cancer types which may facilitate the development or improvement of tumor immunotherapy.How to cite: Zhao L, Yang X, Feng C, et al. Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layer. Electron J Biotechnol 2021;50.https://doi.org/10.1016/j.ejbt.2021.01.001http://www.sciencedirect.com/science/article/pii/S0717345821000014Alternative splicingBreast cancerCross talkT cellsTranscriptomic datasetTranslation initiation |
| spellingShingle | Lina Zhao Xi Yang Chun Feng Yue Wang Qing Wang Jiahong Pei Jinting Wu Shuaiying Li Honglei Zhang Xianbao Cao Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layer Electronic Journal of Biotechnology Alternative splicing Breast cancer Cross talk T cells Transcriptomic dataset Translation initiation |
| title | Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layer |
| title_full | Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layer |
| title_fullStr | Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layer |
| title_full_unstemmed | Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layer |
| title_short | Triple-negative breast cancer cells respond to T cells severely at the alternative splicing layer |
| title_sort | triple negative breast cancer cells respond to t cells severely at the alternative splicing layer |
| topic | Alternative splicing Breast cancer Cross talk T cells Transcriptomic dataset Translation initiation |
| url | http://www.sciencedirect.com/science/article/pii/S0717345821000014 |
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