Cost-Effective PCR-Based Identification of <i>Tunga penetrans</i> (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material

Cost-Effective PCR-Based Identification of <i>Tunga penetrans</i> (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material

Tungiasis is a neglected tropical disease caused by skin-penetrating female <i>Tunga penetrans</i> fleas. Although tungiasis causes severe health problems, its ecology is poorly understood and morphological descriptions of the larvae are unavailable. To identify <i>T. penetrans<...

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Bibliographic Details
Main Authors: Billy L. Amugune, Abneel K. Matharu, Paul Ouma, Francis Mutebi, Lynne Elson, Ulrike Fillinger, Jürgen Krücken
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:Insects
Subjects:
Tungiasis
<i>Tunga penetrans</i>
molecular entomology
DNA isolation
Phusion<sup>®</sup> polymerase
FIREpol<sup>®</sup> Taq polymerase
Online Access:https://www.mdpi.com/2075-4450/14/1/5

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