Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathway
This study aimed to explore the role and key point of EtMIC4 EGF-like recombinant protein in regulating the apoptosis of Eimeria tenella host cells via the epidermal growth factor receptor (EGFR) pathway. The cells were treated with EtMIC4 EGF-like protein, EGFR-specific siRNA, or both. Infection an...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122003662 |
| _version_ | 1811183462095257600 |
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| author | Xue-song Zhang Yong-juan Zhao Yu Zhang Tong Xu Kai-ling Cui Bu-ting Duan Xiao-ling Lv Li Zhang Zhi-yong Xu Rui Bai Ming-xue Zheng |
| author_facet | Xue-song Zhang Yong-juan Zhao Yu Zhang Tong Xu Kai-ling Cui Bu-ting Duan Xiao-ling Lv Li Zhang Zhi-yong Xu Rui Bai Ming-xue Zheng |
| author_sort | Xue-song Zhang |
| collection | DOAJ |
| description | This study aimed to explore the role and key point of EtMIC4 EGF-like recombinant protein in regulating the apoptosis of Eimeria tenella host cells via the epidermal growth factor receptor (EGFR) pathway. The cells were treated with EtMIC4 EGF-like protein, EGFR-specific siRNA, or both. Infection and apoptosis rates as well as dynamic changes in the key genes and proteins of the EGFR signaling pathway in the host cells were determined. Results showed that the E. tenella and EtMIC4 EGF-like group had the highest infection rate (P < 0.01). In cells treated with EtMIC4 EGF-like for 4 to 24 h, the apoptosis rate was significantly decreased (P < 0.01) and the relative mRNA expression and protein phosphorylation levels of EGFR, protein kinase B (AKT), and extracellular regulated protein kinases (ERK) were significantly increased (P < 0.01). In E. tenella sporozoites infected for 4 to 96 h, the rate of host cell apoptosis induced by E. tenella infection was significantly (P < 0.01) reduced by EtMIC4 EGF-like. The relative mRNA expression and protein phosphorylation levels of EGFR, AKT, and ERK in the host cells of E. tenella + EtMIC4 EGF-like group were significantly increased (P < 0.01). These results indicated that E. tenella could activate the EGFR pathway through EtMIC4 EGF-like and regulate the expression of key genes in the AKT and ERK signaling pathways, thereby inhibiting cell apoptosis. |
| first_indexed | 2024-04-11T09:47:35Z |
| format | Article |
| id | doaj.art-926f67079b5e4395a2c583a03fa8d3ce |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-11T09:47:35Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-926f67079b5e4395a2c583a03fa8d3ce2022-12-22T04:30:55ZengElsevierPoultry Science0032-57912022-10-0110110102075Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathwayXue-song Zhang0Yong-juan Zhao1Yu Zhang2Tong Xu3Kai-ling Cui4Bu-ting Duan5Xiao-ling Lv6Li Zhang7Zhi-yong Xu8Rui Bai9Ming-xue Zheng10College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, China; School of Food and Environment, Jinzhong College of Information, Taigu, Jinzhong, 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 030801, China; Corresponding author:This study aimed to explore the role and key point of EtMIC4 EGF-like recombinant protein in regulating the apoptosis of Eimeria tenella host cells via the epidermal growth factor receptor (EGFR) pathway. The cells were treated with EtMIC4 EGF-like protein, EGFR-specific siRNA, or both. Infection and apoptosis rates as well as dynamic changes in the key genes and proteins of the EGFR signaling pathway in the host cells were determined. Results showed that the E. tenella and EtMIC4 EGF-like group had the highest infection rate (P < 0.01). In cells treated with EtMIC4 EGF-like for 4 to 24 h, the apoptosis rate was significantly decreased (P < 0.01) and the relative mRNA expression and protein phosphorylation levels of EGFR, protein kinase B (AKT), and extracellular regulated protein kinases (ERK) were significantly increased (P < 0.01). In E. tenella sporozoites infected for 4 to 96 h, the rate of host cell apoptosis induced by E. tenella infection was significantly (P < 0.01) reduced by EtMIC4 EGF-like. The relative mRNA expression and protein phosphorylation levels of EGFR, AKT, and ERK in the host cells of E. tenella + EtMIC4 EGF-like group were significantly increased (P < 0.01). These results indicated that E. tenella could activate the EGFR pathway through EtMIC4 EGF-like and regulate the expression of key genes in the AKT and ERK signaling pathways, thereby inhibiting cell apoptosis.http://www.sciencedirect.com/science/article/pii/S0032579122003662Eimeria tenellaEtMIC4 EGF-likeapoptosisEGFR pathway |
| spellingShingle | Xue-song Zhang Yong-juan Zhao Yu Zhang Tong Xu Kai-ling Cui Bu-ting Duan Xiao-ling Lv Li Zhang Zhi-yong Xu Rui Bai Ming-xue Zheng Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathway Poultry Science Eimeria tenella EtMIC4 EGF-like apoptosis EGFR pathway |
| title | Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathway |
| title_full | Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathway |
| title_fullStr | Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathway |
| title_full_unstemmed | Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathway |
| title_short | Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells via the EGFR pathway |
| title_sort | role of etmic4 egf like in regulating the apoptosis of eimeria tenella host cells via the egfr pathway |
| topic | Eimeria tenella EtMIC4 EGF-like apoptosis EGFR pathway |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122003662 |
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