Overexpression of the <i>Panax</i> <i>ginseng</i> <i>CYP703</i> Alters Cutin Composition of Reproductive Tissues in Arabidopsis
Cytochrome P450 (CYP) catalyzes a wide variety of monooxygenation reactions in plant primary and secondary metabolisms. Land plants contain CYP703, belonging to the CYP71 clan, which catalyzes the biochemical pathway of fatty acid hydroxylation, especially in male reproductive tissues. Korean/Asian...
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MDPI AG
2022-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/3/383 |
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| author | Jihyun Kim Jeniffer Silva Chanwoo Park Younghun Kim Nayeon Park Johan Sukweenadhi Junping Yu Jianxin Shi Dabing Zhang Keun Ki Kim Hong-Joo Son Hyeon Cheal Park Chang-Oh Hong Kwang Min Lee Yu-Jin Kim |
| author_facet | Jihyun Kim Jeniffer Silva Chanwoo Park Younghun Kim Nayeon Park Johan Sukweenadhi Junping Yu Jianxin Shi Dabing Zhang Keun Ki Kim Hong-Joo Son Hyeon Cheal Park Chang-Oh Hong Kwang Min Lee Yu-Jin Kim |
| author_sort | Jihyun Kim |
| collection | DOAJ |
| description | Cytochrome P450 (CYP) catalyzes a wide variety of monooxygenation reactions in plant primary and secondary metabolisms. Land plants contain CYP703, belonging to the CYP71 clan, which catalyzes the biochemical pathway of fatty acid hydroxylation, especially in male reproductive tissues. Korean/Asian ginseng (<i>Panax ginseng</i> Meyer) has been regarded as one of important medicinal plant for a long time, however the molecular mechanism is less known on its development. In this study, we identified and characterized a <i>CYP703A</i> gene in <i>P. ginseng</i> (<i>PgCYP703A4</i>), regarding reproductive development. <i>PgCYP703A4</i> shared a high-sequence identity (81–83%) with predicted amino acid as CYP703 in <i>Dancus carota</i>, <i>Pistacia vera</i>, and <i>Camellia sinensis</i> as well as 76% of amino acid sequence identity with reported <i>CYP703</i> in <i>Arabidopsis thaliana</i> and 75% with <i>Oryza sativa</i>. Amino acid alignment and phylogenetic comparison of <i>P. ginseng</i> with higher plants and known <i>A. thaliana</i> members clearly distinguish the CYP703 members, each containing the AATDTS oxygen binding motif and PERH as a clade signature. The expression of <i>PgCYP704B1</i> was only detected in <i>P. ginseng</i> flower buds, particularly in meiotic cells and the tapetum layer of developing anther, indicating the conserved role on male reproduction with At- and Os- CYP703. To acquire the clue of function, we transformed the <i>PgCYP703A4</i> in <i>A. thaliana</i>. Independent overexpressing lines (<i>PgCYP703A4</i>ox) increased silique size and seed number, and altered the contents of fatty acids composition of cutin monomer in the siliques. Our results indicate that <i>PgCYP703A4</i> is involved in fatty acid hydroxylation which affects cutin production and fruit size. |
| first_indexed | 2024-03-09T23:18:43Z |
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| last_indexed | 2024-03-09T23:18:43Z |
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| spelling | doaj.art-9277471eb36d48e980858d941988cf9a2023-11-23T17:31:03ZengMDPI AGPlants2223-77472022-01-0111338310.3390/plants11030383Overexpression of the <i>Panax</i> <i>ginseng</i> <i>CYP703</i> Alters Cutin Composition of Reproductive Tissues in ArabidopsisJihyun Kim0Jeniffer Silva1Chanwoo Park2Younghun Kim3Nayeon Park4Johan Sukweenadhi5Junping Yu6Jianxin Shi7Dabing Zhang8Keun Ki Kim9Hong-Joo Son10Hyeon Cheal Park11Chang-Oh Hong12Kwang Min Lee13Yu-Jin Kim14Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Research and Development, The Bridge Biofoundry, Ciudad de Saber, Clayton 0843-03081, PanamaDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Plant Biotechnology, Faculty of Biotechnology, Universitas Surabaya, Raya Kalirungkut, Kalirungkut, Surabaya 60294, East Java, IndonesiaKey Laboratory of Biotechnology Shaanxi Province, College of Life Sciences, Chinese Education Ministry’s Key Laboratory of Western Resources and Modern Biotechnology, Northwest University, Xi’an 710069, ChinaJoint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaJoint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaCytochrome P450 (CYP) catalyzes a wide variety of monooxygenation reactions in plant primary and secondary metabolisms. Land plants contain CYP703, belonging to the CYP71 clan, which catalyzes the biochemical pathway of fatty acid hydroxylation, especially in male reproductive tissues. Korean/Asian ginseng (<i>Panax ginseng</i> Meyer) has been regarded as one of important medicinal plant for a long time, however the molecular mechanism is less known on its development. In this study, we identified and characterized a <i>CYP703A</i> gene in <i>P. ginseng</i> (<i>PgCYP703A4</i>), regarding reproductive development. <i>PgCYP703A4</i> shared a high-sequence identity (81–83%) with predicted amino acid as CYP703 in <i>Dancus carota</i>, <i>Pistacia vera</i>, and <i>Camellia sinensis</i> as well as 76% of amino acid sequence identity with reported <i>CYP703</i> in <i>Arabidopsis thaliana</i> and 75% with <i>Oryza sativa</i>. Amino acid alignment and phylogenetic comparison of <i>P. ginseng</i> with higher plants and known <i>A. thaliana</i> members clearly distinguish the CYP703 members, each containing the AATDTS oxygen binding motif and PERH as a clade signature. The expression of <i>PgCYP704B1</i> was only detected in <i>P. ginseng</i> flower buds, particularly in meiotic cells and the tapetum layer of developing anther, indicating the conserved role on male reproduction with At- and Os- CYP703. To acquire the clue of function, we transformed the <i>PgCYP703A4</i> in <i>A. thaliana</i>. Independent overexpressing lines (<i>PgCYP703A4</i>ox) increased silique size and seed number, and altered the contents of fatty acids composition of cutin monomer in the siliques. Our results indicate that <i>PgCYP703A4</i> is involved in fatty acid hydroxylation which affects cutin production and fruit size.https://www.mdpi.com/2223-7747/11/3/383cytochrome P450reproductive tissues<i>PgCYP703A4</i>fatty acidreproduction<i>Panax ginseng</i> |
| spellingShingle | Jihyun Kim Jeniffer Silva Chanwoo Park Younghun Kim Nayeon Park Johan Sukweenadhi Junping Yu Jianxin Shi Dabing Zhang Keun Ki Kim Hong-Joo Son Hyeon Cheal Park Chang-Oh Hong Kwang Min Lee Yu-Jin Kim Overexpression of the <i>Panax</i> <i>ginseng</i> <i>CYP703</i> Alters Cutin Composition of Reproductive Tissues in Arabidopsis Plants cytochrome P450 reproductive tissues <i>PgCYP703A4</i> fatty acid reproduction <i>Panax ginseng</i> |
| title | Overexpression of the <i>Panax</i> <i>ginseng</i> <i>CYP703</i> Alters Cutin Composition of Reproductive Tissues in Arabidopsis |
| title_full | Overexpression of the <i>Panax</i> <i>ginseng</i> <i>CYP703</i> Alters Cutin Composition of Reproductive Tissues in Arabidopsis |
| title_fullStr | Overexpression of the <i>Panax</i> <i>ginseng</i> <i>CYP703</i> Alters Cutin Composition of Reproductive Tissues in Arabidopsis |
| title_full_unstemmed | Overexpression of the <i>Panax</i> <i>ginseng</i> <i>CYP703</i> Alters Cutin Composition of Reproductive Tissues in Arabidopsis |
| title_short | Overexpression of the <i>Panax</i> <i>ginseng</i> <i>CYP703</i> Alters Cutin Composition of Reproductive Tissues in Arabidopsis |
| title_sort | overexpression of the i panax i i ginseng i i cyp703 i alters cutin composition of reproductive tissues in arabidopsis |
| topic | cytochrome P450 reproductive tissues <i>PgCYP703A4</i> fatty acid reproduction <i>Panax ginseng</i> |
| url | https://www.mdpi.com/2223-7747/11/3/383 |
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