The Distribution and Diversity of Bartonella Species in Rodents and Their Ectoparasites across Thailand.
Our study highlights the surveillance of Bartonella species among rodents and their associated ectoparasites (ticks, fleas, lice, and mites) in several regions across Thailand. A total of 619 rodents and 554 pooled ectoparasites (287 mite pools, 62 flea pools, 35 louse pools, and 170 tick pools) wer...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4617648?pdf=render |
| _version_ | 1811225131321655296 |
|---|---|
| author | Kewalin Klangthong Sommai Promsthaporn Surachai Leepitakrat Anthony L Schuster Patrick W McCardle Michael Kosoy Ratree Takhampunya |
| author_facet | Kewalin Klangthong Sommai Promsthaporn Surachai Leepitakrat Anthony L Schuster Patrick W McCardle Michael Kosoy Ratree Takhampunya |
| author_sort | Kewalin Klangthong |
| collection | DOAJ |
| description | Our study highlights the surveillance of Bartonella species among rodents and their associated ectoparasites (ticks, fleas, lice, and mites) in several regions across Thailand. A total of 619 rodents and 554 pooled ectoparasites (287 mite pools, 62 flea pools, 35 louse pools, and 170 tick pools) were collected from 8 provinces within 4 regions of Thailand. Bandicota indica (279), Rattus rattus (163), and R. exulans (96) were the most prevalent species of rats collected in this study. Real-time PCR assay targeting Bartonella-specific ssrA gene was used for screening and each positive sample was confirmed by PCR using nuoG gene. The prevalence of Bartonella DNA in rodent (around 17%) was recorded in all regions. The highest prevalence of Bartonella species was found in B. savilei and R. rattus with the rate of 35.7% (5/14) and 32.5% (53/163), respectively. High prevalence of Bartonella-positive rodent was also found in B. indica (15.1%, 42/279), and R. norvegicus (12.5%, 5/40). In contrast, the prevalence of Bartonella species in ectoparasites collected from the rats varied significantly according to types of ectoparasites. A high prevalence of Bartonella DNA was found in louse pools (Polyplax spp. and Hoplopleura spp., 57.1%) and flea pools (Xenopsylla cheopis, 25.8%), while a low prevalence was found in pools of mites (Leptotrombidium spp. and Ascoschoengastia spp., 1.7%) and ticks (Haemaphysalis spp., 3.5%). Prevalence of Bartonella DNA in ectoparasites collected from Bartonella-positive rodents (19.4%) was significantly higher comparing to ectoparasites from Bartonella-negative rodents (8.7%). The phylogenetic analysis of 41 gltA sequences of 16 Bartonella isolates from rodent blood and 25 Bartonella-positive ectoparasites revealed a wide range of diversity among Bartonella species with a majority of sequences (61.0%) belonging to Bartonella elizabethae complex (11 rodents, 1 mite pool, and 5 louse pools), while the remaining sequences were identical to B. phoceensis (17.1%, 1 mite pool, 5 louse pools, and 1 tick pool), B. coopersplainensis (19.5%, 5 rodents, 1 louse pool, and 2 tick pools), and one previously unidentified Bartonella species (2.4%, 1 louse pool). |
| first_indexed | 2024-04-12T09:01:44Z |
| format | Article |
| id | doaj.art-92783ed93bfa4fc3967ce74b7833a7fe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T09:01:44Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-92783ed93bfa4fc3967ce74b7833a7fe2022-12-22T03:39:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011010e014085610.1371/journal.pone.0140856The Distribution and Diversity of Bartonella Species in Rodents and Their Ectoparasites across Thailand.Kewalin KlangthongSommai PromsthapornSurachai LeepitakratAnthony L SchusterPatrick W McCardleMichael KosoyRatree TakhampunyaOur study highlights the surveillance of Bartonella species among rodents and their associated ectoparasites (ticks, fleas, lice, and mites) in several regions across Thailand. A total of 619 rodents and 554 pooled ectoparasites (287 mite pools, 62 flea pools, 35 louse pools, and 170 tick pools) were collected from 8 provinces within 4 regions of Thailand. Bandicota indica (279), Rattus rattus (163), and R. exulans (96) were the most prevalent species of rats collected in this study. Real-time PCR assay targeting Bartonella-specific ssrA gene was used for screening and each positive sample was confirmed by PCR using nuoG gene. The prevalence of Bartonella DNA in rodent (around 17%) was recorded in all regions. The highest prevalence of Bartonella species was found in B. savilei and R. rattus with the rate of 35.7% (5/14) and 32.5% (53/163), respectively. High prevalence of Bartonella-positive rodent was also found in B. indica (15.1%, 42/279), and R. norvegicus (12.5%, 5/40). In contrast, the prevalence of Bartonella species in ectoparasites collected from the rats varied significantly according to types of ectoparasites. A high prevalence of Bartonella DNA was found in louse pools (Polyplax spp. and Hoplopleura spp., 57.1%) and flea pools (Xenopsylla cheopis, 25.8%), while a low prevalence was found in pools of mites (Leptotrombidium spp. and Ascoschoengastia spp., 1.7%) and ticks (Haemaphysalis spp., 3.5%). Prevalence of Bartonella DNA in ectoparasites collected from Bartonella-positive rodents (19.4%) was significantly higher comparing to ectoparasites from Bartonella-negative rodents (8.7%). The phylogenetic analysis of 41 gltA sequences of 16 Bartonella isolates from rodent blood and 25 Bartonella-positive ectoparasites revealed a wide range of diversity among Bartonella species with a majority of sequences (61.0%) belonging to Bartonella elizabethae complex (11 rodents, 1 mite pool, and 5 louse pools), while the remaining sequences were identical to B. phoceensis (17.1%, 1 mite pool, 5 louse pools, and 1 tick pool), B. coopersplainensis (19.5%, 5 rodents, 1 louse pool, and 2 tick pools), and one previously unidentified Bartonella species (2.4%, 1 louse pool).http://europepmc.org/articles/PMC4617648?pdf=render |
| spellingShingle | Kewalin Klangthong Sommai Promsthaporn Surachai Leepitakrat Anthony L Schuster Patrick W McCardle Michael Kosoy Ratree Takhampunya The Distribution and Diversity of Bartonella Species in Rodents and Their Ectoparasites across Thailand. PLoS ONE |
| title | The Distribution and Diversity of Bartonella Species in Rodents and Their Ectoparasites across Thailand. |
| title_full | The Distribution and Diversity of Bartonella Species in Rodents and Their Ectoparasites across Thailand. |
| title_fullStr | The Distribution and Diversity of Bartonella Species in Rodents and Their Ectoparasites across Thailand. |
| title_full_unstemmed | The Distribution and Diversity of Bartonella Species in Rodents and Their Ectoparasites across Thailand. |
| title_short | The Distribution and Diversity of Bartonella Species in Rodents and Their Ectoparasites across Thailand. |
| title_sort | distribution and diversity of bartonella species in rodents and their ectoparasites across thailand |
| url | http://europepmc.org/articles/PMC4617648?pdf=render |
| work_keys_str_mv | AT kewalinklangthong thedistributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT sommaipromsthaporn thedistributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT surachaileepitakrat thedistributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT anthonylschuster thedistributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT patrickwmccardle thedistributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT michaelkosoy thedistributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT ratreetakhampunya thedistributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT kewalinklangthong distributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT sommaipromsthaporn distributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT surachaileepitakrat distributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT anthonylschuster distributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT patrickwmccardle distributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT michaelkosoy distributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand AT ratreetakhampunya distributionanddiversityofbartonellaspeciesinrodentsandtheirectoparasitesacrossthailand |