Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar muscles
Genes that are expressed in skeletal muscles may play a role in prenatal muscle development and postnatal muscle growth and can be considered candidates for economically important traits. Four porcine genes that were differentially expressed in skeletal muscles of Czech Large White and Wild Boar (SO...
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Czech Academy of Agricultural Sciences
2014-03-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201403-0003_polymorphism-linkage-mapping-and-association-analysis-with-carcass-traits-of-four-porcine-candidate-genes-sel.php |
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| author | P. Chalupová V. Dvořáková A. Knoll A. Stratil H. Bartenschlager R. Stupka J. Čítek M. Šprysl A. Palánová P. Horák H. Geldermann |
| author_facet | P. Chalupová V. Dvořáková A. Knoll A. Stratil H. Bartenschlager R. Stupka J. Čítek M. Šprysl A. Palánová P. Horák H. Geldermann |
| author_sort | P. Chalupová |
| collection | DOAJ |
| description | Genes that are expressed in skeletal muscles may play a role in prenatal muscle development and postnatal muscle growth and can be considered candidates for economically important traits. Four porcine genes that were differentially expressed in skeletal muscles of Czech Large White and Wild Boar (SORT1, EMP3, IL18, and BTG2) were selected to search for polymorphism, linkage assignment, and association analysis with carcass traits. Through comparative sequencing of portions of the genes numerous polymorphisms were revealed (SORT1 - 21, EMP3 - 6, IL18 - 41, BTG2 - 9). Linkage analysis in a Meishan × Pietrain F2 pedigree showed the positions of the genes relative to other genes and markers on the respective chromosomes - SORT1 on SSC4, EMP3 on SSC6, IL18 and BTG2 on SSC9. Preliminary association analysis in pig commercial crosses with selected SNPs showed associations with several carcass traits at nominal P value of < 0.05, which may indicate their involvement in muscle growth and fat deposition. The tested polymorphisms may not be causal for the associations, but they may be in linkage disequilibrium with causative mutations. |
| first_indexed | 2024-04-10T08:26:18Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:26:18Z |
| publishDate | 2014-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-927cc24698594e42a0beb6ccacca60a02023-02-23T03:33:12ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092014-03-0159311612710.17221/7291-CJAScjs-201403-0003Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar musclesP. Chalupová0V. Dvořáková1A. Knoll2A. Stratil3H. Bartenschlager4R. Stupka5J. Čítek6M. Šprysl7A. Palánová8P. Horák9H. Geldermann10Department of Morphology, Physiology and Animal Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicInstitute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech RepublicDepartment of Morphology, Physiology and Animal Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech RepublicInstitute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech RepublicDepartment of Animal Breeding and Biotechnology, University of Hohenheim, Stuttgart, GermanyDepartment of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicInstitute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech RepublicInstitute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech RepublicDepartment of Animal Breeding and Biotechnology, University of Hohenheim, Stuttgart, GermanyGenes that are expressed in skeletal muscles may play a role in prenatal muscle development and postnatal muscle growth and can be considered candidates for economically important traits. Four porcine genes that were differentially expressed in skeletal muscles of Czech Large White and Wild Boar (SORT1, EMP3, IL18, and BTG2) were selected to search for polymorphism, linkage assignment, and association analysis with carcass traits. Through comparative sequencing of portions of the genes numerous polymorphisms were revealed (SORT1 - 21, EMP3 - 6, IL18 - 41, BTG2 - 9). Linkage analysis in a Meishan × Pietrain F2 pedigree showed the positions of the genes relative to other genes and markers on the respective chromosomes - SORT1 on SSC4, EMP3 on SSC6, IL18 and BTG2 on SSC9. Preliminary association analysis in pig commercial crosses with selected SNPs showed associations with several carcass traits at nominal P value of < 0.05, which may indicate their involvement in muscle growth and fat deposition. The tested polymorphisms may not be causal for the associations, but they may be in linkage disequilibrium with causative mutations.https://cjas.agriculturejournals.cz/artkey/cjs-201403-0003_polymorphism-linkage-mapping-and-association-analysis-with-carcass-traits-of-four-porcine-candidate-genes-sel.phppigsingle nucleotide polymorphismdna markersdna sequencingpcr-rflpsort1emp3il18btg2 |
| spellingShingle | P. Chalupová V. Dvořáková A. Knoll A. Stratil H. Bartenschlager R. Stupka J. Čítek M. Šprysl A. Palánová P. Horák H. Geldermann Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar muscles Czech Journal of Animal Science pig single nucleotide polymorphism dna markers dna sequencing pcr-rflp sort1 emp3 il18 btg2 |
| title | Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar muscles |
| title_full | Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar muscles |
| title_fullStr | Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar muscles |
| title_full_unstemmed | Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar muscles |
| title_short | Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar muscles |
| title_sort | polymorphism linkage mapping and association analysis with carcass traits of four porcine candidate genes selected from gene expression profiles of czech large white and wild boar muscles |
| topic | pig single nucleotide polymorphism dna markers dna sequencing pcr-rflp sort1 emp3 il18 btg2 |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201403-0003_polymorphism-linkage-mapping-and-association-analysis-with-carcass-traits-of-four-porcine-candidate-genes-sel.php |
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