Effect of Radiation Therapy on Composition of Lymphocyte Populations in Patients with Primary Breast Cancer
Background: Radiation therapy (RT) is an important step in the treatment of primary breast cancer as it is one of the leading contributors to cancer incidence among women. Most patients with this disease acquire radiation-induced lymphopenia in the early post-radiation period; however, little is kno...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/13/9/1399 |
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| author | Irina Kobzeva Tatiana Astrelina Yuliya Suchkova Tatyana Malivanova Daria Usupzhanova Vitaliy Brunchukov Anna Rastorgueva Victoria Nikitina Ekaterina Lubaeva Marina Sukhova Alexey Kirilchev Tatyana Butkova Alexander Izotov Kristina Malsagova Alexander Samoilov Vasiliy Pustovoyt |
| author_facet | Irina Kobzeva Tatiana Astrelina Yuliya Suchkova Tatyana Malivanova Daria Usupzhanova Vitaliy Brunchukov Anna Rastorgueva Victoria Nikitina Ekaterina Lubaeva Marina Sukhova Alexey Kirilchev Tatyana Butkova Alexander Izotov Kristina Malsagova Alexander Samoilov Vasiliy Pustovoyt |
| author_sort | Irina Kobzeva |
| collection | DOAJ |
| description | Background: Radiation therapy (RT) is an important step in the treatment of primary breast cancer as it is one of the leading contributors to cancer incidence among women. Most patients with this disease acquire radiation-induced lymphopenia in the early post-radiation period; however, little is known about the effect of RT on the composition of lymphocyte populations in such patients. This study was aimed at investigating the effect of adjuvant remote RT—performed in the classical mode for patients with primary breast cancer—on the main components of cell-mediated immunity (major lymphocyte populations), including those in patients receiving chemotherapy. Methods: Between 2020 and 2022, 96 patients with stage I–III breast cancer were included in this study. All patients in the final stage of complex treatment received RT via a 3D conformal technique (3DCRT). The clinical target volume of this RT included the breast or chest wall and locoregional lymphatics. Flow cytometry was used to assess the levels and phenotypes of circulating lymphocytes before and after RT (no more than 7 days before and after RT). The evaluation of the impact of polychemotherapy (PCT) was conducted to determine whether it was a risk factor for the onset of radio-induced lymphopenia (RIL) in the context of RT. Results: When assessing the immune status in the general group of patients (n = 96), before the start of adjuvant external beam radiotherapy (EBRT), the average number of lymphocytes was 1.68 ± 0.064 × 10<sup>9</sup>/L; after the course of adjuvant EBRT, it decreased to 1.01 ± 0.044 × 10<sup>9</sup>/L (<i>p</i> < 0.001). When assessing the absolute indicators of cellular immunity in the general group of patients with BC after a course of adjuvant EBRT, significant dynamics were revealed by the changes in all cell populations of lymphocytes (paired <i>t</i>-test, <i>p</i> < 0.05). Conclusion: The adaptive immune system in breast cancer patients changed in the early post-radiation period. The absolute levels of B-, T- and natural killer cells significantly reduced after RT regardless of whether the patients previously underwent chemotherapy courses. RT for patients with primary breast cancer should be considered in clinical management because it significantly alters lymphocyte levels and should be considered when assessing antitumor immunity, as significant changes in T-cell immunity have been observed. In addition, the identified changes are critical if specific targeted therapy or immunotherapy is needed. |
| first_indexed | 2024-03-10T22:34:16Z |
| format | Article |
| id | doaj.art-928434270dc049babf5bd921b233e093 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-10T22:34:16Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
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| series | Journal of Personalized Medicine |
| spelling | doaj.art-928434270dc049babf5bd921b233e0932023-11-19T11:31:42ZengMDPI AGJournal of Personalized Medicine2075-44262023-09-01139139910.3390/jpm13091399Effect of Radiation Therapy on Composition of Lymphocyte Populations in Patients with Primary Breast CancerIrina Kobzeva0Tatiana Astrelina1Yuliya Suchkova2Tatyana Malivanova3Daria Usupzhanova4Vitaliy Brunchukov5Anna Rastorgueva6Victoria Nikitina7Ekaterina Lubaeva8Marina Sukhova9Alexey Kirilchev10Tatyana Butkova11Alexander Izotov12Kristina Malsagova13Alexander Samoilov14Vasiliy Pustovoyt15State Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaInstitute of Biomedical Chemistry, Biobanking Group, 109028 Moscow, RussiaInstitute of Biomedical Chemistry, Biobanking Group, 109028 Moscow, RussiaInstitute of Biomedical Chemistry, Biobanking Group, 109028 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaState Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 141701 Moscow, RussiaBackground: Radiation therapy (RT) is an important step in the treatment of primary breast cancer as it is one of the leading contributors to cancer incidence among women. Most patients with this disease acquire radiation-induced lymphopenia in the early post-radiation period; however, little is known about the effect of RT on the composition of lymphocyte populations in such patients. This study was aimed at investigating the effect of adjuvant remote RT—performed in the classical mode for patients with primary breast cancer—on the main components of cell-mediated immunity (major lymphocyte populations), including those in patients receiving chemotherapy. Methods: Between 2020 and 2022, 96 patients with stage I–III breast cancer were included in this study. All patients in the final stage of complex treatment received RT via a 3D conformal technique (3DCRT). The clinical target volume of this RT included the breast or chest wall and locoregional lymphatics. Flow cytometry was used to assess the levels and phenotypes of circulating lymphocytes before and after RT (no more than 7 days before and after RT). The evaluation of the impact of polychemotherapy (PCT) was conducted to determine whether it was a risk factor for the onset of radio-induced lymphopenia (RIL) in the context of RT. Results: When assessing the immune status in the general group of patients (n = 96), before the start of adjuvant external beam radiotherapy (EBRT), the average number of lymphocytes was 1.68 ± 0.064 × 10<sup>9</sup>/L; after the course of adjuvant EBRT, it decreased to 1.01 ± 0.044 × 10<sup>9</sup>/L (<i>p</i> < 0.001). When assessing the absolute indicators of cellular immunity in the general group of patients with BC after a course of adjuvant EBRT, significant dynamics were revealed by the changes in all cell populations of lymphocytes (paired <i>t</i>-test, <i>p</i> < 0.05). Conclusion: The adaptive immune system in breast cancer patients changed in the early post-radiation period. The absolute levels of B-, T- and natural killer cells significantly reduced after RT regardless of whether the patients previously underwent chemotherapy courses. RT for patients with primary breast cancer should be considered in clinical management because it significantly alters lymphocyte levels and should be considered when assessing antitumor immunity, as significant changes in T-cell immunity have been observed. In addition, the identified changes are critical if specific targeted therapy or immunotherapy is needed.https://www.mdpi.com/2075-4426/13/9/1399lymphopeniabreast cancerradiotherapylymphocytes |
| spellingShingle | Irina Kobzeva Tatiana Astrelina Yuliya Suchkova Tatyana Malivanova Daria Usupzhanova Vitaliy Brunchukov Anna Rastorgueva Victoria Nikitina Ekaterina Lubaeva Marina Sukhova Alexey Kirilchev Tatyana Butkova Alexander Izotov Kristina Malsagova Alexander Samoilov Vasiliy Pustovoyt Effect of Radiation Therapy on Composition of Lymphocyte Populations in Patients with Primary Breast Cancer Journal of Personalized Medicine lymphopenia breast cancer radiotherapy lymphocytes |
| title | Effect of Radiation Therapy on Composition of Lymphocyte Populations in Patients with Primary Breast Cancer |
| title_full | Effect of Radiation Therapy on Composition of Lymphocyte Populations in Patients with Primary Breast Cancer |
| title_fullStr | Effect of Radiation Therapy on Composition of Lymphocyte Populations in Patients with Primary Breast Cancer |
| title_full_unstemmed | Effect of Radiation Therapy on Composition of Lymphocyte Populations in Patients with Primary Breast Cancer |
| title_short | Effect of Radiation Therapy on Composition of Lymphocyte Populations in Patients with Primary Breast Cancer |
| title_sort | effect of radiation therapy on composition of lymphocyte populations in patients with primary breast cancer |
| topic | lymphopenia breast cancer radiotherapy lymphocytes |
| url | https://www.mdpi.com/2075-4426/13/9/1399 |
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