The first human case report of molecularly confirmed co-infection of Brucella melitensis and Coxiella burnetii: A case report
Co-infection with Brucella melitensis and Coxiella burnetii has been rarely reported. To date, there are only two co-infection case reports from Croatia and China which diagnosed the infections mainly through the use of serological tests. In this report, we present the first case of molecularly conf...
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| Formato: | Artigo |
| Idioma: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
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| Acceso en liña: | http://www.sciencedirect.com/science/article/pii/S2405844024057165 |
| _version_ | 1827261783678648320 |
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| author | Onjira Mangkalamanee Suwatchareeporn Rotcheewaphan Pawat Phuensan Teerada Ponpinit Thiravat Hemachudha Pattama Torvorapanit |
| author_facet | Onjira Mangkalamanee Suwatchareeporn Rotcheewaphan Pawat Phuensan Teerada Ponpinit Thiravat Hemachudha Pattama Torvorapanit |
| author_sort | Onjira Mangkalamanee |
| collection | DOAJ |
| description | Co-infection with Brucella melitensis and Coxiella burnetii has been rarely reported. To date, there are only two co-infection case reports from Croatia and China which diagnosed the infections mainly through the use of serological tests. In this report, we present the first case of molecularly confirmed B. melitensis bacteremia and C. burnetii spondylodiscitis co-infection in a goat dairy farmer who presented with lumbosacral spondylodiscitis and bilateral psoas abscesses. From the blood culture, B. melitensis was identified by using 16S rRNA gene sequencing and specific PCR. Lumbar bone tissue was found to be positive for C. burnetii using multiplex real-time PCR and was confirmed with a positive result from conventional PCR which detected the infection through the identification of the IS1111 gene. The patient's condition improved after decompressive laminectomy was performed and administration of antibiotics regimen: intravenous gentamicin, oral rifampicin, and oral doxycycline. From our case, it is important to raise awareness of this underreported co-infection with multiple zoonotic diseases, especially Q fever and brucellosis, which share the same exposure risk. Moreover, we also emphasize the use of advanced molecular techniques to improve the diagnostic efficiency and reduce the use of time-consuming procedures among patients who are continuously exposed to such risk factors in areas with high seroprevalence of these zoonotic diseases. |
| first_indexed | 2024-04-24T07:37:31Z |
| format | Article |
| id | doaj.art-928440d468af45ad85595f3ce0dc72d3 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2025-03-22T03:03:52Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-928440d468af45ad85595f3ce0dc72d32024-05-01T05:09:17ZengElsevierHeliyon2405-84402024-04-01108e29685The first human case report of molecularly confirmed co-infection of Brucella melitensis and Coxiella burnetii: A case reportOnjira Mangkalamanee0Suwatchareeporn Rotcheewaphan1Pawat Phuensan2Teerada Ponpinit3Thiravat Hemachudha4Pattama Torvorapanit5Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, And King Chulalongkorn Memorial Hospital, Thai Red Cross Society, 1873, Rama IV Road, Pathum Wan, Bangkok, 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, 1873, Rama IV Road, Pathum Wan, Bangkok, 10330, Thailand; Center of Excellence of Antimicrobial Stewardship, Chulalongkorn University, 1873, Rama IV Road, Pathum Wan, Bangkok, 10330, ThailandDivision of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, And King Chulalongkorn Memorial Hospital, Thai Red Cross Society, 1873, Rama IV Road, Pathum Wan, Bangkok, 10330, Thailand; Division of Hospital and Ambulatory Medicine, Department of Medicine, Faculty of Medicine, Chulalongkorn University, And King Chulalongkorn Memorial Hospital, Thai Red Cross Society, 1873, Rama IV Road, Pathum Wan, Bangkok, 10330, ThailandThai Red Cross Emerging Infectious Diseases Health Science Centre, World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital-The Thai Red Cross Society, Bangkok 10330, ThailandThai Red Cross Emerging Infectious Diseases Health Science Centre, World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital-The Thai Red Cross Society, Bangkok 10330, Thailand; Division of Neurology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDivision of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, And King Chulalongkorn Memorial Hospital, Thai Red Cross Society, 1873, Rama IV Road, Pathum Wan, Bangkok, 10330, Thailand; Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, 1873, Rama IV Road, Pathum Wan, Bangkok, 10330, Thailand; Corresponding author. Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Emerging Infectious Diseases Clinical Center and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, 1873, Rama IV Road, Pathum Wan, Bangkok, 10330, Thailand.Co-infection with Brucella melitensis and Coxiella burnetii has been rarely reported. To date, there are only two co-infection case reports from Croatia and China which diagnosed the infections mainly through the use of serological tests. In this report, we present the first case of molecularly confirmed B. melitensis bacteremia and C. burnetii spondylodiscitis co-infection in a goat dairy farmer who presented with lumbosacral spondylodiscitis and bilateral psoas abscesses. From the blood culture, B. melitensis was identified by using 16S rRNA gene sequencing and specific PCR. Lumbar bone tissue was found to be positive for C. burnetii using multiplex real-time PCR and was confirmed with a positive result from conventional PCR which detected the infection through the identification of the IS1111 gene. The patient's condition improved after decompressive laminectomy was performed and administration of antibiotics regimen: intravenous gentamicin, oral rifampicin, and oral doxycycline. From our case, it is important to raise awareness of this underreported co-infection with multiple zoonotic diseases, especially Q fever and brucellosis, which share the same exposure risk. Moreover, we also emphasize the use of advanced molecular techniques to improve the diagnostic efficiency and reduce the use of time-consuming procedures among patients who are continuously exposed to such risk factors in areas with high seroprevalence of these zoonotic diseases.http://www.sciencedirect.com/science/article/pii/S2405844024057165Brucella melitensisCoxiella burnetiiQ feverCo-infectionSpondylodiscitisCase report |
| spellingShingle | Onjira Mangkalamanee Suwatchareeporn Rotcheewaphan Pawat Phuensan Teerada Ponpinit Thiravat Hemachudha Pattama Torvorapanit The first human case report of molecularly confirmed co-infection of Brucella melitensis and Coxiella burnetii: A case report Heliyon Brucella melitensis Coxiella burnetii Q fever Co-infection Spondylodiscitis Case report |
| title | The first human case report of molecularly confirmed co-infection of Brucella melitensis and Coxiella burnetii: A case report |
| title_full | The first human case report of molecularly confirmed co-infection of Brucella melitensis and Coxiella burnetii: A case report |
| title_fullStr | The first human case report of molecularly confirmed co-infection of Brucella melitensis and Coxiella burnetii: A case report |
| title_full_unstemmed | The first human case report of molecularly confirmed co-infection of Brucella melitensis and Coxiella burnetii: A case report |
| title_short | The first human case report of molecularly confirmed co-infection of Brucella melitensis and Coxiella burnetii: A case report |
| title_sort | first human case report of molecularly confirmed co infection of brucella melitensis and coxiella burnetii a case report |
| topic | Brucella melitensis Coxiella burnetii Q fever Co-infection Spondylodiscitis Case report |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024057165 |
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