Methylated Septin9 has moderate diagnostic value in colorectal cancer detection in Chinese population: a multicenter study
Abstract Background The detection rate of methylated Septin9 (mSEPT9) in colorectal cancer (CRC) is varied greatly across the studies. This study aimed to evaluate the diagnostic ability of mSEPT9 in CRC, and compare the diagnostic efficacy with fecal immunochemical test (FIT). Methods 326 subjects...
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| Format: | Article |
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BMC
2022-05-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-022-02313-x |
| _version_ | 1818203743424872448 |
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| author | Dong-cheng Lu Qi-fang Zhang Li Li Xian-ke Luo Bin Liang Yi-han Lu Bang-li Hu Hai-xing Jiang |
| author_facet | Dong-cheng Lu Qi-fang Zhang Li Li Xian-ke Luo Bin Liang Yi-han Lu Bang-li Hu Hai-xing Jiang |
| author_sort | Dong-cheng Lu |
| collection | DOAJ |
| description | Abstract Background The detection rate of methylated Septin9 (mSEPT9) in colorectal cancer (CRC) is varied greatly across the studies. This study aimed to evaluate the diagnostic ability of mSEPT9 in CRC, and compare the diagnostic efficacy with fecal immunochemical test (FIT). Methods 326 subjects from four centers were prospectively recruited, including 179 CRC and 147 non-CRC subjects. The plasma was collected for mSEPT9 and CEA, AFP, CA125, CA153 and CA199 test, and fecal samples for FIT tests. Sensitivity, specificity and area under the curve (AUC) of receiver operating characteristic curve were calculated to evaluate the diagnostic value of each biomarker. Results The positive rate in mSEPT9 and FIT, and the level of CEA, CA125 and CA199 were significantly higher in CRC compared with non-CRC subjects. The mSEPT9 positive rate was not associated with TNM stage and tumor stage. The sensitivity, specificity and AUC of mSEPT9 in diagnostic CRC were 0.77, 0.88 and 0.82, respectively, while the value in FIT was 0.88, 0.80 and 0.83, respectively. mSEPT9 and FIT have higher AUC value than that of CEA, CA125 and CA199. Combination of both mSEPT9 and FIT positive increased sensitivity and AUC to 0.98 and 0.83, respectively, but the specificity was declined. mSEPT9 has a slightly low sensitivity in diagnosis of colon cancer (0.87) compared with rectal cancer (0.93). Conclusion mSEPT9 demonstrated moderate diagnostic value in CRC detection, which was similar to the FIT but superior to the CEA, CA125 and CA199. Combination of mSEPT9 and FIT further improved diagnostic sensitivity in CRC. Trial registration: ChiCTR2000038319. |
| first_indexed | 2024-12-12T03:30:12Z |
| format | Article |
| id | doaj.art-928a99056134483488b7c22c8341a639 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-12-12T03:30:12Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-928a99056134483488b7c22c8341a6392022-12-22T00:39:56ZengBMCBMC Gastroenterology1471-230X2022-05-012211910.1186/s12876-022-02313-xMethylated Septin9 has moderate diagnostic value in colorectal cancer detection in Chinese population: a multicenter studyDong-cheng Lu0Qi-fang Zhang1Li Li2Xian-ke Luo3Bin Liang4Yi-han Lu5Bang-li Hu6Hai-xing Jiang7Department of Gastroenterology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Gastroenterology, Nanxishan Hospital of Guangxi Zhuang Autonomous RegionDepartment of Gastroenterology, Jiangbin Hospital of Guangxi Zhuang Autonomous RegionDepartment of Gastroenterology, Minzu Hospital of Guangxi Zhuang Autonomous RegionDepartment of Gastroenterology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Gastroenterology, First Affiliated Hospital of Guangxi Medical UniversityDepartment of Research, Guangxi Medical University Cancer HospitalDepartment of Gastroenterology, First Affiliated Hospital of Guangxi Medical UniversityAbstract Background The detection rate of methylated Septin9 (mSEPT9) in colorectal cancer (CRC) is varied greatly across the studies. This study aimed to evaluate the diagnostic ability of mSEPT9 in CRC, and compare the diagnostic efficacy with fecal immunochemical test (FIT). Methods 326 subjects from four centers were prospectively recruited, including 179 CRC and 147 non-CRC subjects. The plasma was collected for mSEPT9 and CEA, AFP, CA125, CA153 and CA199 test, and fecal samples for FIT tests. Sensitivity, specificity and area under the curve (AUC) of receiver operating characteristic curve were calculated to evaluate the diagnostic value of each biomarker. Results The positive rate in mSEPT9 and FIT, and the level of CEA, CA125 and CA199 were significantly higher in CRC compared with non-CRC subjects. The mSEPT9 positive rate was not associated with TNM stage and tumor stage. The sensitivity, specificity and AUC of mSEPT9 in diagnostic CRC were 0.77, 0.88 and 0.82, respectively, while the value in FIT was 0.88, 0.80 and 0.83, respectively. mSEPT9 and FIT have higher AUC value than that of CEA, CA125 and CA199. Combination of both mSEPT9 and FIT positive increased sensitivity and AUC to 0.98 and 0.83, respectively, but the specificity was declined. mSEPT9 has a slightly low sensitivity in diagnosis of colon cancer (0.87) compared with rectal cancer (0.93). Conclusion mSEPT9 demonstrated moderate diagnostic value in CRC detection, which was similar to the FIT but superior to the CEA, CA125 and CA199. Combination of mSEPT9 and FIT further improved diagnostic sensitivity in CRC. Trial registration: ChiCTR2000038319.https://doi.org/10.1186/s12876-022-02313-xColorectal cancerSeptin9MethylationFecal immunochemical testDiagnosis |
| spellingShingle | Dong-cheng Lu Qi-fang Zhang Li Li Xian-ke Luo Bin Liang Yi-han Lu Bang-li Hu Hai-xing Jiang Methylated Septin9 has moderate diagnostic value in colorectal cancer detection in Chinese population: a multicenter study BMC Gastroenterology Colorectal cancer Septin9 Methylation Fecal immunochemical test Diagnosis |
| title | Methylated Septin9 has moderate diagnostic value in colorectal cancer detection in Chinese population: a multicenter study |
| title_full | Methylated Septin9 has moderate diagnostic value in colorectal cancer detection in Chinese population: a multicenter study |
| title_fullStr | Methylated Septin9 has moderate diagnostic value in colorectal cancer detection in Chinese population: a multicenter study |
| title_full_unstemmed | Methylated Septin9 has moderate diagnostic value in colorectal cancer detection in Chinese population: a multicenter study |
| title_short | Methylated Septin9 has moderate diagnostic value in colorectal cancer detection in Chinese population: a multicenter study |
| title_sort | methylated septin9 has moderate diagnostic value in colorectal cancer detection in chinese population a multicenter study |
| topic | Colorectal cancer Septin9 Methylation Fecal immunochemical test Diagnosis |
| url | https://doi.org/10.1186/s12876-022-02313-x |
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