Nanoparticle-Mediated Radiotherapy: Unraveling Dose Enhancement and Apoptotic Responses in Cancer and Normal Cell Lines
Cervical cancer remains a pressing global health concern, necessitating advanced therapeutic strategies. Radiotherapy, a fundamental treatment modality, has faced challenges such as targeted dose deposition and radiation exposure to healthy tissues, limiting optimal outcomes. To address these hurdle...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/12/1720 |
| _version_ | 1797381878763749376 |
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| author | Maria Anthi Kouri Ellas Spyratou Maria-Eleni Kalkou Georgios Patatoukas Evangelia Angelopoulou Ioanna Tremi Sophia Havaki Vassilis G. Gorgoulis Vassilis Kouloulias Kalliopi Platoni Efstathios P. Efstathopoulos |
| author_facet | Maria Anthi Kouri Ellas Spyratou Maria-Eleni Kalkou Georgios Patatoukas Evangelia Angelopoulou Ioanna Tremi Sophia Havaki Vassilis G. Gorgoulis Vassilis Kouloulias Kalliopi Platoni Efstathios P. Efstathopoulos |
| author_sort | Maria Anthi Kouri |
| collection | DOAJ |
| description | Cervical cancer remains a pressing global health concern, necessitating advanced therapeutic strategies. Radiotherapy, a fundamental treatment modality, has faced challenges such as targeted dose deposition and radiation exposure to healthy tissues, limiting optimal outcomes. To address these hurdles, nanomaterials, specifically gold nanoparticles (AuNPs), have emerged as a promising avenue. This study delves into the realm of cervical cancer radiotherapy through the meticulous exploration of AuNPs’ impact. Utilizing ex vivo experiments involving cell lines, this research dissected intricate radiobiological interactions. Detailed scrutiny of cell survival curves, dose enhancement factors (DEFs), and apoptosis in both cancer and normal cervical cells revealed profound insights. The outcomes showcased the substantial enhancement of radiation responses in cancer cells following AuNP treatment, resulting in heightened cell death and apoptotic levels. Significantly, the most pronounced effects were observed 24 h post-irradiation, emphasizing the pivotal role of timing in AuNPs’ efficacy. Importantly, AuNPs exhibited targeted precision, selectively impacting cancer cells while preserving normal cells. This study illuminates the potential of AuNPs as potent radiosensitizers in cervical cancer therapy, offering a tailored and efficient approach. Through meticulous ex vivo experimentation, this research expands our comprehension of the complex dynamics between AuNPs and cells, laying the foundation for their optimized clinical utilization. |
| first_indexed | 2024-03-08T20:57:52Z |
| format | Article |
| id | doaj.art-928ec6f8278744858c5fcd2c38d26c56 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-08T20:57:52Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-928ec6f8278744858c5fcd2c38d26c562023-12-22T13:55:54ZengMDPI AGBiomolecules2218-273X2023-11-011312172010.3390/biom13121720Nanoparticle-Mediated Radiotherapy: Unraveling Dose Enhancement and Apoptotic Responses in Cancer and Normal Cell LinesMaria Anthi Kouri0Ellas Spyratou1Maria-Eleni Kalkou2Georgios Patatoukas3Evangelia Angelopoulou4Ioanna Tremi5Sophia Havaki6Vassilis G. Gorgoulis7Vassilis Kouloulias8Kalliopi Platoni9Efstathios P. Efstathopoulos102nd Department of Radiology, Medical School, Attikon University Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece2nd Department of Radiology, Medical School, Attikon University Hospital, National and Kapodistrian University of Athens, 11527 Athens, GreeceMedical School, National and Kapodistrian University of Athens, 75 Mikras Asias Str., 11527 Athens, Greece2nd Department of Radiology, Medical School, Attikon University Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece2nd Department of Pathology, School of Medicine, Attikon University Hospital, National and Kapodistrian University of Athens, 12462 Athens, GreeceMolecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, GreeceMolecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, GreeceMolecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece2nd Department of Radiology, Medical School, Attikon University Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece2nd Department of Radiology, Medical School, Attikon University Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece2nd Department of Radiology, Medical School, Attikon University Hospital, National and Kapodistrian University of Athens, 11527 Athens, GreeceCervical cancer remains a pressing global health concern, necessitating advanced therapeutic strategies. Radiotherapy, a fundamental treatment modality, has faced challenges such as targeted dose deposition and radiation exposure to healthy tissues, limiting optimal outcomes. To address these hurdles, nanomaterials, specifically gold nanoparticles (AuNPs), have emerged as a promising avenue. This study delves into the realm of cervical cancer radiotherapy through the meticulous exploration of AuNPs’ impact. Utilizing ex vivo experiments involving cell lines, this research dissected intricate radiobiological interactions. Detailed scrutiny of cell survival curves, dose enhancement factors (DEFs), and apoptosis in both cancer and normal cervical cells revealed profound insights. The outcomes showcased the substantial enhancement of radiation responses in cancer cells following AuNP treatment, resulting in heightened cell death and apoptotic levels. Significantly, the most pronounced effects were observed 24 h post-irradiation, emphasizing the pivotal role of timing in AuNPs’ efficacy. Importantly, AuNPs exhibited targeted precision, selectively impacting cancer cells while preserving normal cells. This study illuminates the potential of AuNPs as potent radiosensitizers in cervical cancer therapy, offering a tailored and efficient approach. Through meticulous ex vivo experimentation, this research expands our comprehension of the complex dynamics between AuNPs and cells, laying the foundation for their optimized clinical utilization.https://www.mdpi.com/2218-273X/13/12/1720gold nanoparticlesradiation therapySiHaCaskiHCK1Tcellular apoptosis |
| spellingShingle | Maria Anthi Kouri Ellas Spyratou Maria-Eleni Kalkou Georgios Patatoukas Evangelia Angelopoulou Ioanna Tremi Sophia Havaki Vassilis G. Gorgoulis Vassilis Kouloulias Kalliopi Platoni Efstathios P. Efstathopoulos Nanoparticle-Mediated Radiotherapy: Unraveling Dose Enhancement and Apoptotic Responses in Cancer and Normal Cell Lines Biomolecules gold nanoparticles radiation therapy SiHa Caski HCK1T cellular apoptosis |
| title | Nanoparticle-Mediated Radiotherapy: Unraveling Dose Enhancement and Apoptotic Responses in Cancer and Normal Cell Lines |
| title_full | Nanoparticle-Mediated Radiotherapy: Unraveling Dose Enhancement and Apoptotic Responses in Cancer and Normal Cell Lines |
| title_fullStr | Nanoparticle-Mediated Radiotherapy: Unraveling Dose Enhancement and Apoptotic Responses in Cancer and Normal Cell Lines |
| title_full_unstemmed | Nanoparticle-Mediated Radiotherapy: Unraveling Dose Enhancement and Apoptotic Responses in Cancer and Normal Cell Lines |
| title_short | Nanoparticle-Mediated Radiotherapy: Unraveling Dose Enhancement and Apoptotic Responses in Cancer and Normal Cell Lines |
| title_sort | nanoparticle mediated radiotherapy unraveling dose enhancement and apoptotic responses in cancer and normal cell lines |
| topic | gold nanoparticles radiation therapy SiHa Caski HCK1T cellular apoptosis |
| url | https://www.mdpi.com/2218-273X/13/12/1720 |
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