Phenotypical Differences between <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies
We previously showed that <i>L.</i> (<i>Leishmania</i>) <i>amazonensis</i> promastigotes and amastigotes of the PH8 strain generated larger lesions in mice than LV79, and that lesion-derived amastigotes from the two strains differ in their proteomes. We recently r...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2076-0817/12/2/173 |
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| author | Fabia Tomie Tano Erich Loza Telleria Felipe Dutra Rêgo Felipe Soares Coelho Eloiza de Rezende Rodrigo Pedro Soares Yara Maria Traub-Cseko Beatriz Simonsen Stolf |
| author_facet | Fabia Tomie Tano Erich Loza Telleria Felipe Dutra Rêgo Felipe Soares Coelho Eloiza de Rezende Rodrigo Pedro Soares Yara Maria Traub-Cseko Beatriz Simonsen Stolf |
| author_sort | Fabia Tomie Tano |
| collection | DOAJ |
| description | We previously showed that <i>L.</i> (<i>Leishmania</i>) <i>amazonensis</i> promastigotes and amastigotes of the PH8 strain generated larger lesions in mice than LV79, and that lesion-derived amastigotes from the two strains differ in their proteomes. We recently reported that PH8 promastigotes are more phagocytized by macrophages. Promastigotes’ membrane-enriched proteomes showed several differences, and samples of each strain clustered based on proteomes. In this paper, we show phenotypic differences between PH8 and LV79 promastigotes that may explain the higher virulence of PH8. We compared in vitro macrophage infections by day 4 (early) and day 6 (late stationary phase) cultures, resistance to complement, and LPG characteristics. PH8 promastigotes showed a higher infectivity and were more resistant to murine complement. LPG was different between the strains, which may influence the interaction with macrophages and survival to complement. We compared the infection of the permissive vector <i>Lutzomyia longipalpis</i>. PH8 was more abundant in the vector’s gut 72 h after feeding, which is a moment where blood digestion is finished and the parasites are exposed to the gut environment. Our results indicate that PH8 promastigotes are more infective, more resistant to complement, and infect the permissive vector more efficiently. These data suggest that PH8 is probably better adapted to the sand fly and more prone to survive in the vertebrate host. |
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| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T08:19:04Z |
| publishDate | 2023-01-01 |
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| series | Pathogens |
| spelling | doaj.art-9292815a50e54a76b395795efcb1b6412023-11-16T22:33:02ZengMDPI AGPathogens2076-08172023-01-0112217310.3390/pathogens12020173Phenotypical Differences between <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand FliesFabia Tomie Tano0Erich Loza Telleria1Felipe Dutra Rêgo2Felipe Soares Coelho3Eloiza de Rezende4Rodrigo Pedro Soares5Yara Maria Traub-Cseko6Beatriz Simonsen Stolf7Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, SP, BrazilLaboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, BrazilBiotechnology Applied to Pathogens (BAP), Instituto René Rachou, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte 30000-000, MG, BrazilLaboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, BrazilDepartment of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, SP, BrazilBiotechnology Applied to Pathogens (BAP), Instituto René Rachou, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte 30000-000, MG, BrazilLaboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, BrazilDepartment of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, SP, BrazilWe previously showed that <i>L.</i> (<i>Leishmania</i>) <i>amazonensis</i> promastigotes and amastigotes of the PH8 strain generated larger lesions in mice than LV79, and that lesion-derived amastigotes from the two strains differ in their proteomes. We recently reported that PH8 promastigotes are more phagocytized by macrophages. Promastigotes’ membrane-enriched proteomes showed several differences, and samples of each strain clustered based on proteomes. In this paper, we show phenotypic differences between PH8 and LV79 promastigotes that may explain the higher virulence of PH8. We compared in vitro macrophage infections by day 4 (early) and day 6 (late stationary phase) cultures, resistance to complement, and LPG characteristics. PH8 promastigotes showed a higher infectivity and were more resistant to murine complement. LPG was different between the strains, which may influence the interaction with macrophages and survival to complement. We compared the infection of the permissive vector <i>Lutzomyia longipalpis</i>. PH8 was more abundant in the vector’s gut 72 h after feeding, which is a moment where blood digestion is finished and the parasites are exposed to the gut environment. Our results indicate that PH8 promastigotes are more infective, more resistant to complement, and infect the permissive vector more efficiently. These data suggest that PH8 is probably better adapted to the sand fly and more prone to survive in the vertebrate host.https://www.mdpi.com/2076-0817/12/2/173<i>L.</i> (<i>L.</i>) <i>amazonensis</i>virulenceinfectioncomplementLPGsand fly |
| spellingShingle | Fabia Tomie Tano Erich Loza Telleria Felipe Dutra Rêgo Felipe Soares Coelho Eloiza de Rezende Rodrigo Pedro Soares Yara Maria Traub-Cseko Beatriz Simonsen Stolf Phenotypical Differences between <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies Pathogens <i>L.</i> (<i>L.</i>) <i>amazonensis</i> virulence infection complement LPG sand fly |
| title | Phenotypical Differences between <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies |
| title_full | Phenotypical Differences between <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies |
| title_fullStr | Phenotypical Differences between <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies |
| title_full_unstemmed | Phenotypical Differences between <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies |
| title_short | Phenotypical Differences between <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies |
| title_sort | phenotypical differences between i leishmania i i leishmania i i amazonensis i ph8 and lv79 strains may impact survival in mammal host and in phlebotomine sand flies |
| topic | <i>L.</i> (<i>L.</i>) <i>amazonensis</i> virulence infection complement LPG sand fly |
| url | https://www.mdpi.com/2076-0817/12/2/173 |
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