Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy
Background: Radiation induced bystander effects (RIBEs) occurs in unirradiated cells exhibiting indirect biological effect as a consequence of signals from other irradiated cells in the population. <br />Objective: In this study, bystander effects in MCF-7 breast cancer cells and hFOB 1.19 nor...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2020-06-01
|
| Series: | Journal of Biomedical Physics and Engineering |
| Subjects: | |
| Online Access: | https://jbpe.sums.ac.ir/article_46374_622ab521da234d5e597f8e308b75ad6d.pdf |
| _version_ | 1818408787419070464 |
|---|---|
| author | N H Mohd Zainudin R Abdullah W N Rahman |
| author_facet | N H Mohd Zainudin R Abdullah W N Rahman |
| author_sort | N H Mohd Zainudin |
| collection | DOAJ |
| description | Background: Radiation induced bystander effects (RIBEs) occurs in unirradiated cells exhibiting indirect biological effect as a consequence of signals from other irradiated cells in the population. <br />Objective: In this study, bystander effects in MCF-7 breast cancer cells and hFOB 1.19 normal osteoblast cells irradiated with gamma emitting HDR Brachytherapy Ir-192 source were investigated.<br />Material and Methods: In this in-vitro study, bystander effect stimulation was conducted using medium transfer technique of irradiated cells to the non-irradiated bystander cells. Cell viability, reactive oxygen species (ROS) generation and colony forming assay was employed to evaluate the effect. <br />Results: Results indicate that the exposure to the medium irradiated MCF-7 induced significant bystander killing and decreased the survival fraction of bystander MCF-7 and hFOB from 1.19 to 81.70 % and 65.44 %, respectively. A significant decrease in survival fraction was observed for hFOB 1.19 bystander cells (p < 0.05). We found that the rate of hFOB 1.19 cell growth significantly decreases to 85.5% when added with media from irradiated cells. The ROS levels of bystander cells for both cell lines were observed to have an increase even after 4 h of treatment. Our results suggest the presence of bystander effects in unirradiated cells exposed to the irradiated medium. <br />Conclusion: These data provide evidence that irradiated MCF-7 breast cancer cells can induce bystander death in unirradiated MCF-7 and hFOB 1.19 bystander cells. Increase in cell death could also be mediated by the ROS generation during the irradiation with HDR brachytherapy. |
| first_indexed | 2024-12-14T09:49:17Z |
| format | Article |
| id | doaj.art-9298ffbe814940b5a4d6cc97299bfee3 |
| institution | Directory Open Access Journal |
| issn | 2251-7200 2251-7200 |
| language | English |
| last_indexed | 2024-12-14T09:49:17Z |
| publishDate | 2020-06-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Biomedical Physics and Engineering |
| spelling | doaj.art-9298ffbe814940b5a4d6cc97299bfee32022-12-21T23:07:34ZengShiraz University of Medical SciencesJournal of Biomedical Physics and Engineering2251-72002251-72002020-06-0110331932810.31661/jbpe.v0i0.113546374Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-BrachytherapyN H Mohd Zainudin0R Abdullah1W N Rahman2PhD student, Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia|PhD student, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Gong Badak Campus, Kuala Terengganu, MalaysiaPhD, Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia |PhD, Department of Nuclear Medicine, Radiotherapy and Oncology, Hospital Universiti Sains Malaysia (HUSM), Kelantan, MalaysiaPhD, Department of Nuclear Medicine, Radiotherapy and Oncology, Hospital Universiti Sains Malaysia (HUSM), Kelantan, MalaysiaBackground: Radiation induced bystander effects (RIBEs) occurs in unirradiated cells exhibiting indirect biological effect as a consequence of signals from other irradiated cells in the population. <br />Objective: In this study, bystander effects in MCF-7 breast cancer cells and hFOB 1.19 normal osteoblast cells irradiated with gamma emitting HDR Brachytherapy Ir-192 source were investigated.<br />Material and Methods: In this in-vitro study, bystander effect stimulation was conducted using medium transfer technique of irradiated cells to the non-irradiated bystander cells. Cell viability, reactive oxygen species (ROS) generation and colony forming assay was employed to evaluate the effect. <br />Results: Results indicate that the exposure to the medium irradiated MCF-7 induced significant bystander killing and decreased the survival fraction of bystander MCF-7 and hFOB from 1.19 to 81.70 % and 65.44 %, respectively. A significant decrease in survival fraction was observed for hFOB 1.19 bystander cells (p < 0.05). We found that the rate of hFOB 1.19 cell growth significantly decreases to 85.5% when added with media from irradiated cells. The ROS levels of bystander cells for both cell lines were observed to have an increase even after 4 h of treatment. Our results suggest the presence of bystander effects in unirradiated cells exposed to the irradiated medium. <br />Conclusion: These data provide evidence that irradiated MCF-7 breast cancer cells can induce bystander death in unirradiated MCF-7 and hFOB 1.19 bystander cells. Increase in cell death could also be mediated by the ROS generation during the irradiation with HDR brachytherapy.https://jbpe.sums.ac.ir/article_46374_622ab521da234d5e597f8e308b75ad6d.pdfbreast cancerosteoblastsbrachytherapybystander effectradiation effect |
| spellingShingle | N H Mohd Zainudin R Abdullah W N Rahman Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy Journal of Biomedical Physics and Engineering breast cancer osteoblasts brachytherapy bystander effect radiation effect |
| title | Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy |
| title_full | Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy |
| title_fullStr | Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy |
| title_full_unstemmed | Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy |
| title_short | Bystander Effect Induced in Breast Cancer (MCF-7) and Human Osteoblast Cell Lines (hFOB 1.19) with HDR-Brachytherapy |
| title_sort | bystander effect induced in breast cancer mcf 7 and human osteoblast cell lines hfob 1 19 with hdr brachytherapy |
| topic | breast cancer osteoblasts brachytherapy bystander effect radiation effect |
| url | https://jbpe.sums.ac.ir/article_46374_622ab521da234d5e597f8e308b75ad6d.pdf |
| work_keys_str_mv | AT nhmohdzainudin bystandereffectinducedinbreastcancermcf7andhumanosteoblastcelllineshfob119withhdrbrachytherapy AT rabdullah bystandereffectinducedinbreastcancermcf7andhumanosteoblastcelllineshfob119withhdrbrachytherapy AT wnrahman bystandereffectinducedinbreastcancermcf7andhumanosteoblastcelllineshfob119withhdrbrachytherapy |