Optimization of o-cresol degrading microorganism and kinetics of degradation
In the present study, Pseudomonas monteilii CR13 isolated from petroleum contaminated soil demonstrated the highest specific o-cresol degradation rate at all tested o-cresol concentrations and also was not disturbed by the starting substrate concentration used (o-cresol-500 mg/L). After a serial tra...
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Universitas Gadjah Mada
2018-11-01
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| Series: | Indonesian Journal of Chemistry |
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| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/35326 |
| _version_ | 1818989856592756736 |
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| author | Krishnan Nhattuketty Shainy Rajamanickam Usha |
| author_facet | Krishnan Nhattuketty Shainy Rajamanickam Usha |
| author_sort | Krishnan Nhattuketty Shainy |
| collection | DOAJ |
| description | In the present study, Pseudomonas monteilii CR13 isolated from petroleum contaminated soil demonstrated the highest specific o-cresol degradation rate at all tested o-cresol concentrations and also was not disturbed by the starting substrate concentration used (o-cresol-500 mg/L). After a serial transfer of the isolate into a series of increasing o-cresol level, the organism demonstrated significant improvement on degradation ability up to 3000 mg/L. The optimum condition for the cell mass increase and biodegradation of o-cresol by Pseudomonas monteilii was in the minimal mineral medium of 3 at a pH of 6.5 and temperature 30 °C, stirring velocity of 160 rpm, and the substrate concentration of 500 mg/L. The biodegradation kinetic study was carried out by bacteria in different initial substrate concentrations (500–3000 mg/L). In the present test the μmax, Ks and the μ were found 0.332 h-1, 0.166 mg/L and 0.0282 mg/L for 500 mg/L of o-cresol, respectively. The organism is highly promising and could be used to remove high concentrations of o-cresol from highly polluted aquatic and soil regions. The cells could be immobilized on a suitable matrix and the efficiency of degradation could be effectively improved. |
| first_indexed | 2024-12-20T19:45:08Z |
| format | Article |
| id | doaj.art-929ab04c897b4c718cab73a8e0b4fb67 |
| institution | Directory Open Access Journal |
| issn | 1411-9420 2460-1578 |
| language | English |
| last_indexed | 2024-12-20T19:45:08Z |
| publishDate | 2018-11-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj.art-929ab04c897b4c718cab73a8e0b4fb672022-12-21T19:28:26ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782018-11-0118470270810.22146/ijc.3532621918Optimization of o-cresol degrading microorganism and kinetics of degradationKrishnan Nhattuketty Shainy0Rajamanickam Usha1Department of Microbiology, Safi Center for Scientific Research, Vazhayoor East, Malappuram – 673633, Kerala, IndiaDepartment of Microbiology, Karpagam Academy for Higher Education, Eachinary, Coimbatore – 641021, Tamil Nadu, IndiaIn the present study, Pseudomonas monteilii CR13 isolated from petroleum contaminated soil demonstrated the highest specific o-cresol degradation rate at all tested o-cresol concentrations and also was not disturbed by the starting substrate concentration used (o-cresol-500 mg/L). After a serial transfer of the isolate into a series of increasing o-cresol level, the organism demonstrated significant improvement on degradation ability up to 3000 mg/L. The optimum condition for the cell mass increase and biodegradation of o-cresol by Pseudomonas monteilii was in the minimal mineral medium of 3 at a pH of 6.5 and temperature 30 °C, stirring velocity of 160 rpm, and the substrate concentration of 500 mg/L. The biodegradation kinetic study was carried out by bacteria in different initial substrate concentrations (500–3000 mg/L). In the present test the μmax, Ks and the μ were found 0.332 h-1, 0.166 mg/L and 0.0282 mg/L for 500 mg/L of o-cresol, respectively. The organism is highly promising and could be used to remove high concentrations of o-cresol from highly polluted aquatic and soil regions. The cells could be immobilized on a suitable matrix and the efficiency of degradation could be effectively improved.https://jurnal.ugm.ac.id/ijc/article/view/35326biodegradationo-cresolPseudomonas monteiliipetroleum contaminated soil |
| spellingShingle | Krishnan Nhattuketty Shainy Rajamanickam Usha Optimization of o-cresol degrading microorganism and kinetics of degradation Indonesian Journal of Chemistry biodegradation o-cresol Pseudomonas monteilii petroleum contaminated soil |
| title | Optimization of o-cresol degrading microorganism and kinetics of degradation |
| title_full | Optimization of o-cresol degrading microorganism and kinetics of degradation |
| title_fullStr | Optimization of o-cresol degrading microorganism and kinetics of degradation |
| title_full_unstemmed | Optimization of o-cresol degrading microorganism and kinetics of degradation |
| title_short | Optimization of o-cresol degrading microorganism and kinetics of degradation |
| title_sort | optimization of o cresol degrading microorganism and kinetics of degradation |
| topic | biodegradation o-cresol Pseudomonas monteilii petroleum contaminated soil |
| url | https://jurnal.ugm.ac.id/ijc/article/view/35326 |
| work_keys_str_mv | AT krishnannhattukettyshainy optimizationofocresoldegradingmicroorganismandkineticsofdegradation AT rajamanickamusha optimizationofocresoldegradingmicroorganismandkineticsofdegradation |