Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis
Abstract We present the first data describing molecular evidence of planktonic coenzyme factor 430 (hereafter referred to as F430), a key signature of methanogenic archaea, obtained from a water column sample in a freshwater lake in Japan. We report concentrations of native F430 ranging from 6.8–35 ...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-11-01
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| Series: | Progress in Earth and Planetary Science |
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| Online Access: | https://doi.org/10.1186/s40645-021-00450-7 |
| _version_ | 1818897293811646464 |
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| author | Atsushi Urai Makoto Matsushita Ho-Dong Park Hiroyuki Imachi Miyuki Ogawara Hiroki Iwata Masanori Kaneko Nanako O. Ogawa Naohiko Ohkouchi Yoshinori Takano |
| author_facet | Atsushi Urai Makoto Matsushita Ho-Dong Park Hiroyuki Imachi Miyuki Ogawara Hiroki Iwata Masanori Kaneko Nanako O. Ogawa Naohiko Ohkouchi Yoshinori Takano |
| author_sort | Atsushi Urai |
| collection | DOAJ |
| description | Abstract We present the first data describing molecular evidence of planktonic coenzyme factor 430 (hereafter referred to as F430), a key signature of methanogenic archaea, obtained from a water column sample in a freshwater lake in Japan. We report concentrations of native F430 ranging from 6.8–35 × 102 femto mol g-wet−1 and 8.5–86 × 102 femto mol g-wet−1 in the water column and core-top sediment, respectively. Among these profiles, the epimer F430 of the deactivated chemical formula was very low overall in both planktonic and benthic environments. Small subunit (SSU) rRNA gene sequencing revealed that the planktonic microbial community was clearly different from that of the benthic sediment. Planktonic cyanobacteria (e.g., Microcystis) were predominant in the water column, whereas a wide variety of bacteria and archaea, including methanogens (e.g., Methanobacterium, Methanoregula, Methanothrix, Methanosarcina, and Methanocella), were observed in the core-top sediment. These results suggest that the planktonic cyanobacterial community may be rapidly degraded at the water–sediment interface driven by activated benthic microbial heterotrophic processes. Graphic abstract |
| first_indexed | 2024-12-19T19:13:53Z |
| format | Article |
| id | doaj.art-929b8e60fa194550bca85eaeb2fb1c0f |
| institution | Directory Open Access Journal |
| issn | 2197-4284 |
| language | English |
| last_indexed | 2024-12-19T19:13:53Z |
| publishDate | 2021-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Progress in Earth and Planetary Science |
| spelling | doaj.art-929b8e60fa194550bca85eaeb2fb1c0f2022-12-21T20:09:11ZengSpringerOpenProgress in Earth and Planetary Science2197-42842021-11-018111710.1186/s40645-021-00450-7Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesisAtsushi Urai0Makoto Matsushita1Ho-Dong Park2Hiroyuki Imachi3Miyuki Ogawara4Hiroki Iwata5Masanori Kaneko6Nanako O. Ogawa7Naohiko Ohkouchi8Yoshinori Takano9Biogeochemistry Research Center (BGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)Biogeochemistry Research Center (BGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)Department of Environmental Science, Faculty of Science, Shinshu UniversityInstitute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), JAMSTECInstitute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), JAMSTECDepartment of Environmental Science, Faculty of Science, Shinshu UniversityBiogeochemistry Research Center (BGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)Biogeochemistry Research Center (BGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)Biogeochemistry Research Center (BGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)Biogeochemistry Research Center (BGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)Abstract We present the first data describing molecular evidence of planktonic coenzyme factor 430 (hereafter referred to as F430), a key signature of methanogenic archaea, obtained from a water column sample in a freshwater lake in Japan. We report concentrations of native F430 ranging from 6.8–35 × 102 femto mol g-wet−1 and 8.5–86 × 102 femto mol g-wet−1 in the water column and core-top sediment, respectively. Among these profiles, the epimer F430 of the deactivated chemical formula was very low overall in both planktonic and benthic environments. Small subunit (SSU) rRNA gene sequencing revealed that the planktonic microbial community was clearly different from that of the benthic sediment. Planktonic cyanobacteria (e.g., Microcystis) were predominant in the water column, whereas a wide variety of bacteria and archaea, including methanogens (e.g., Methanobacterium, Methanoregula, Methanothrix, Methanosarcina, and Methanocella), were observed in the core-top sediment. These results suggest that the planktonic cyanobacterial community may be rapidly degraded at the water–sediment interface driven by activated benthic microbial heterotrophic processes. Graphic abstracthttps://doi.org/10.1186/s40645-021-00450-7Planktonic methanogenCoenzyme F430mcrALake SuwaSurface hydrosphere |
| spellingShingle | Atsushi Urai Makoto Matsushita Ho-Dong Park Hiroyuki Imachi Miyuki Ogawara Hiroki Iwata Masanori Kaneko Nanako O. Ogawa Naohiko Ohkouchi Yoshinori Takano Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis Progress in Earth and Planetary Science Planktonic methanogen Coenzyme F430 mcrA Lake Suwa Surface hydrosphere |
| title | Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis |
| title_full | Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis |
| title_fullStr | Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis |
| title_full_unstemmed | Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis |
| title_short | Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis |
| title_sort | detection of planktonic coenzyme factor 430 in a freshwater lake small scale analysis for probing archaeal methanogenesis |
| topic | Planktonic methanogen Coenzyme F430 mcrA Lake Suwa Surface hydrosphere |
| url | https://doi.org/10.1186/s40645-021-00450-7 |
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