C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis
Abstract Background RNA polymerase II plays critical roles in transcription in eukaryotic organisms. C-terminal Domain Phosphatase-like 1 (CPL1) regulates the phosphorylation state of the C-terminal domain of RNA polymerase II subunit B1, which is critical in determining RNA polymerase II activity....
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BMC
2021-09-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07966-8 |
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| author | Chen Yuan Jingya Xu Qianqian Chen Qinggang Liu Yikai Hu Yicheng Jin Cheng Qin |
| author_facet | Chen Yuan Jingya Xu Qianqian Chen Qinggang Liu Yikai Hu Yicheng Jin Cheng Qin |
| author_sort | Chen Yuan |
| collection | DOAJ |
| description | Abstract Background RNA polymerase II plays critical roles in transcription in eukaryotic organisms. C-terminal Domain Phosphatase-like 1 (CPL1) regulates the phosphorylation state of the C-terminal domain of RNA polymerase II subunit B1, which is critical in determining RNA polymerase II activity. CPL1 plays an important role in miRNA biogenesis, plant growth and stress responses. Although cpl1 mutant showes delayed-flowering phenotype, the molecular mechanism behind CPL1’s role in floral transition is still unknown. Results To study the role of CPL1 during the floral transition, we first tested phenotypes of cpl1-3 mutant, which harbors a point-mutation. The cpl1-3 mutant contains a G-to-A transition in the second exon, which results in an amino acid substitution from Glu to Lys (E116K). Further analyses found that the mutated amino acid (Glu) was conserved in these species. As a result, we found that the cpl1-3 mutant experienced delayed flowering under both long- and short-day conditions, and CPL1 is involved in the vernalization pathway. Transcriptome analysis identified 109 genes differentially expressed in the cpl1 mutant, with 2 being involved in floral transition. Differential expression of the two flowering-related DEGs was further validated by qRT-PCR. Conclusions Flowering genetic pathways analysis coupled with transciptomic analysis provides potential genes related to floral transition in the cpl1-3 mutant, and a framework for future studies of the molecular mechanisms behind CPL1’s role in floral transition. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-16T08:22:29Z |
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| spelling | doaj.art-92a08cdc98c841eeb8664b6c3830a9b42022-12-21T22:38:05ZengBMCBMC Genomics1471-21642021-09-012211910.1186/s12864-021-07966-8C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in ArabidopsisChen Yuan0Jingya Xu1Qianqian Chen2Qinggang Liu3Yikai Hu4Yicheng Jin5Cheng Qin6Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal UniversityResearch Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal UniversityResearch Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal UniversityResearch Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal UniversityResearch Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal UniversityDivision of Research and Development, Oriomics IncResearch Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal UniversityAbstract Background RNA polymerase II plays critical roles in transcription in eukaryotic organisms. C-terminal Domain Phosphatase-like 1 (CPL1) regulates the phosphorylation state of the C-terminal domain of RNA polymerase II subunit B1, which is critical in determining RNA polymerase II activity. CPL1 plays an important role in miRNA biogenesis, plant growth and stress responses. Although cpl1 mutant showes delayed-flowering phenotype, the molecular mechanism behind CPL1’s role in floral transition is still unknown. Results To study the role of CPL1 during the floral transition, we first tested phenotypes of cpl1-3 mutant, which harbors a point-mutation. The cpl1-3 mutant contains a G-to-A transition in the second exon, which results in an amino acid substitution from Glu to Lys (E116K). Further analyses found that the mutated amino acid (Glu) was conserved in these species. As a result, we found that the cpl1-3 mutant experienced delayed flowering under both long- and short-day conditions, and CPL1 is involved in the vernalization pathway. Transcriptome analysis identified 109 genes differentially expressed in the cpl1 mutant, with 2 being involved in floral transition. Differential expression of the two flowering-related DEGs was further validated by qRT-PCR. Conclusions Flowering genetic pathways analysis coupled with transciptomic analysis provides potential genes related to floral transition in the cpl1-3 mutant, and a framework for future studies of the molecular mechanisms behind CPL1’s role in floral transition.https://doi.org/10.1186/s12864-021-07966-8C-terminal domain phosphatase-like 1 (CPL1)Floral transitionArabidopsisTranscriptomeVernalization pathway |
| spellingShingle | Chen Yuan Jingya Xu Qianqian Chen Qinggang Liu Yikai Hu Yicheng Jin Cheng Qin C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis BMC Genomics C-terminal domain phosphatase-like 1 (CPL1) Floral transition Arabidopsis Transcriptome Vernalization pathway |
| title | C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis |
| title_full | C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis |
| title_fullStr | C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis |
| title_full_unstemmed | C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis |
| title_short | C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis |
| title_sort | c terminal domain phosphatase like 1 cpl1 is involved in floral transition in arabidopsis |
| topic | C-terminal domain phosphatase-like 1 (CPL1) Floral transition Arabidopsis Transcriptome Vernalization pathway |
| url | https://doi.org/10.1186/s12864-021-07966-8 |
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